Characterization of New Staphylococcus haemolyticus ST42 Populations in Northern Taiwan

2021 ◽  
Author(s):  
Lee-Chung Lin ◽  
Tsui-Ping Liu ◽  
Shih-Cheng Chang ◽  
Jang-Jih Lu
Keyword(s):  
Staphylococcus Haemolyticus ◽  
Northern Taiwan

Phenotypic and Genotypic Characterization of Biofilm Formation in Staphylococcus haemolyticus

2015 ◽  
Vol 70 (6) ◽  
pp. 829-834 ◽  
Author(s):  
Elaine M. Barros ◽  
Moara Lemos ◽  
Thais Souto-Padrón ◽  
Marcia Giambiagi-deMarval
Keyword(s):  
Biofilm Formation ◽  
Staphylococcus Haemolyticus ◽  
Genotypic Characterization ◽  
Phenotypic And Genotypic Characterization

scholarly journals Structural and Biological Characterization of a Capsular Polysaccharide Produced by Staphylococcus haemolyticus

2007 ◽  
Vol 190 (5) ◽  
pp. 1649-1657 ◽  
Author(s):  
Sigrid Flahaut ◽  
Evgeny Vinogradov ◽  
Kathryn A. Kelley ◽  
Shannon Brennan ◽  
Keiichi Hiramatsu ◽  
...  
Keyword(s):  
Capsular Polysaccharide ◽  
In Vitro Assays ◽  
Sugar Residue ◽  
Biological Characterization ◽  
Staphylococcus Haemolyticus ◽  
Acyl Substituent ◽  
Genes Encoding ◽  
Opsonophagocytic Killing

ABSTRACT The DNA sequence of the genome of Staphylococcus haemolyticus JCSC1435 revealed a putative capsule operon composed of 13 genes in tandem. The first seven genes (capABCDEFGSh ) showed ≥57% similarity with the Staphylococcus aureus cap5 or cap8 locus. However, the capHIJKLMSh genes are unique to S. haemolyticus and include genes encoding a putative flippase, an aminotransferase, two glycosyltransferases, and a transcriptional regulator. Capsule-like material was readily apparent by immunoelectron microscopy on bacteria harvested in the postexponential phase of growth. Electron micrographs of a JCSC1435 mutant with a deleted cap region lacked the capsule-like material. Both strains produced small amounts of surface-associated material that reacted with antibodies to polyglutamic acid. S. haemolyticus cap genes were amplified from four of seven clinical isolates of S. haemolyticus from humans, and three of these strains produced a serologically cross-reactive capsular polysaccharide. In vitro assays demonstrated that the acapsular mutant strain showed greater biofilm formation but was more susceptible to complement-mediated opsonophagocytic killing than the parent strain. Structural characterization of capsule purified from S. haemolyticus strain JCSC1435 showed a trisaccharide repeating unit: −3-α-l-FucNAc-3-(2-NAc-4-N-Asp-2,4,6-trideoxy-β-d-Glc)-4-α-d-GlcNAc-. This structure is unique among staphylococcal polysaccharides in that its composition includes a trideoxy sugar residue with aspartic acid as an N-acyl substituent.

Sign in / Sign up

Export Citation Format

Share Document