Occurrence, Antimicrobial Resistance Patterns, and Genetic Characterization of Staphylococcus aureus Isolated from Raw Milk in the Dairy Farms over Two Seasons in China

2020 ◽  
Author(s):  
Cuiping Shi ◽  
Zhongna Yu ◽  
Harvey Ho ◽  
Jun Wang ◽  
Wei Wu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Genetic Characterization ◽  
Dairy Farms ◽  
Raw Milk ◽  
Resistance Patterns

Characterization of Staphylococcus aureus Strains Isolated from Raw Milk Utilized in Small-Scale Artisan Cheese Production

2011 ◽  
Vol 74 (8) ◽  
pp. 1353-1358 ◽  
Author(s):  
DENNIS J. D'AMICO ◽  
CATHERINE W. DONNELLY
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Phenotypic Diversity ◽  
Raw Milk ◽  
Staphylococcal Enterotoxin ◽  
Small Scale ◽  
Enterotoxin Production ◽  
Type C ◽  
Cheese Production

Staphylococcus aureus is an important agent of bacterial mastitis in milking animals and of foodborne intoxication in humans. The purpose of this study was to examine the genetic and phenotypic diversity, enterotoxigenicity, and antimicrobial resistance of S. aureus strains isolated from raw milk used for the production of artisan cheese in Vermont. Cross-tabulations revealed that the 16 ribotypes identified among the 90 milk isolates examined were typically associated with a specific animal species and that more than half of these ribotypes were unique to individual farms. In general, specific EcoRI ribotypes were commonly associated with specific phenotypical characteristics, including staphylococcal enterotoxin production or the lack thereof. Limited antimicrobial resistance was observed among the isolates, with resistance to ampicillin (12.51%) or penicillin (17.04%) most common. Two isolates of the same ribotype obtained from the same farm were resistant to oxacillin with 2% NaCl. More than half (52.22%) of isolates produced toxin, and 31 of the 32 isolates solely produced staphylococcal enterotoxin type C. Although these data demonstrate that S. aureus strains found in raw milk intended for artisan cheese manufacture are capable of enterotoxin production, staphylococcal enterotoxin C is not typically linked to foodborne illness. Because S. aureus is a common contaminant of cheese, an understanding of the ecology of this pathogen and of the antimicrobial susceptibility and toxigenicity of various strains will ultimately contribute to the development of control practices needed to enhance the safety of artisan and farmstead cheese production.

Phylogeny Characterization of Seb Gene Encoding Enterotoxins in Staphylococcus aureus Isolated from Raw Milk and Cheese

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Fatima N. Aziz ◽  
Laith Abdul Hassan Mohammed-Jawad
Keyword(s):  
Staphylococcus Aureus ◽  
Food Poisoning ◽  
Raw Milk ◽  
Staphylococcal Enterotoxin B ◽  
Human Pathogen ◽  
Conventional Pcr ◽  
Biochemical Tests ◽  
Specific Primers ◽  
Gene Encoding

Food poisoning due to the bacteria is a big global problem in economically and human's health. This problem refers to an illness which is due to infection or the toxin exists in nature and the food that use. Milk is considered a nutritious food because it contains proteins and vitamins. The aim of this study is to detect and phylogeny characterization of staphylococcal enterotoxin B gene (Seb). A total of 200 milk and cheese samples were screened. One hundred ten isolates of Staphylococcus aureus pre-confirmed using selective and differential media with biochemical tests. Genomic DNA was extracted from the isolates and the SEB gene detects using conventional PCR with specific primers. Three staphylococcus aureus isolates were found to be positive for Seb gene using PCR and confirmed by sequencing. Sequence homology showed variety range of identity starting from (100% to 38%). Phylogenetic tree analyses show that samples (6 and 5) are correlated with S. epidermidis. This study discovered that isolates (A6-RLQ and A5-RLQ) are significantly clustered in a group with non- human pathogen Staphylococcus agnetis.

scholarly journals Emerging Options for the Diagnosis of Bacterial Infections and the Characterization of Antimicrobial Resistance

2021 ◽  
Vol 22 (1) ◽  
pp. 456
Author(s):  
Simone Rentschler ◽  
Lars Kaiser ◽  
Hans-Peter Deigner
Keyword(s):  
Antimicrobial Resistance ◽  
Bacterial Infections ◽  
Point Of Care ◽  
Rapid Identification ◽  
Adequate Treatment ◽  
Resistance Patterns ◽  
Detection And Diagnosis ◽  
Patient Prognosis ◽  
Point Of Care Detection

Precise and rapid identification and characterization of pathogens and antimicrobial resistance patterns are critical for the adequate treatment of infections, which represent an increasing problem in intensive care medicine. The current situation remains far from satisfactory in terms of turnaround times and overall efficacy. Application of an ineffective antimicrobial agent or the unnecessary use of broad-spectrum antibiotics worsens the patient prognosis and further accelerates the generation of resistant mutants. Here, we provide an overview that includes an evaluation and comparison of existing tools used to diagnose bacterial infections, together with a consideration of the underlying molecular principles and technologies. Special emphasis is placed on emerging developments that may lead to significant improvements in point of care detection and diagnosis of multi-resistant pathogens, and new directions that may be used to guide antibiotic therapy.

scholarly journals Characterization of cefotaxime resistant Escherichia coli isolated from broiler farms in Ecuador

2018 ◽  
Author(s):  
Christian Vinueza-Burgos ◽  
David Ortega-Paredes ◽  
Cristian Narváez ◽  
Lieven De Zutter ◽  
Jeannete Zurita
Keyword(s):  
Antimicrobial Resistance ◽  
Latin American ◽  
Production Systems ◽  
Diffusion Method ◽  
Poultry Production ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Resistance Patterns ◽  
Latin American Countries

AbstractAntimicrobial resistance (AR) is a worldwide concern. Up to a 160% increase in antibiotic usage in food animals is expected in Latin American countries. The poultry industry is an increasingly important segment of food production and contributor to AR. The objective of this study was to evaluate the prevalence, AR patterns and the characterization of relevant resistance genes in Extended Spectrum β-lactamases (ESBL) and AmpC E. coli from large poultry farms in Ecuador. Sampling was performed from June 2013 to July 2014 in 6 slaughterhouses that slaughter broilers from 115 farms totaling 384 flocks. Each sample of collected caeca was streaked onto TBX agar supplemented with cefotaxime (3 mg/l). In total, 176 isolates were analyzed for antimicrobial resistance patterns by the disk diffusion method and for blaCTX-M, blaTEM, blaCMY, blaSHV, blaKPC, and mcr-1 by PCR and sequencing. ESBL and AmpC E. coli were found in 362 flocks (94.3%) from 112 farms (97.4%). We found that 98.3% of the isolates were multi-resistant to antibiotics. Low resistance was observed for ertapenem and nitrofurantoin. The most prevalent ESBL genes were the blaCTX-M (90.9%) blaCTX-M-65, blaCTX-M-55 and blaCTX-M-3 alleles. Most of the AmpC strains presented the blaCMY-2 gene. Three isolates showed the mcr-1 gene. Poultry production systems represent a hotspot for antimicrobial resistance in Ecuador, possibly mediated by the extensive use of antibiotics. Monitoring this sector in national and regional plans of antimicrobial resistance surveillance should therefore be considered.

scholarly journals Prevalence and antibiotic susceptibility profile of Staphylococcus aureus from milk and milk products in Nasarawa State, Nigeria

2020 ◽  
Vol 18 (1) ◽  
pp. 1-12
Author(s):  
A. Yakubu ◽  
I.O Abdullahi ◽  
C.Z. Whong ◽  
B. Olayinka
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Susceptibility ◽  
Hazard Analysis ◽  
Raw Milk ◽  
Fermented Milk ◽  
Milk Products ◽  
Milk Samples ◽  
Critical Control Points ◽  
Agar Disc ◽  
Resistance Patterns

This study determined the prevalence and antibiotic susceptibility profile of Staphylococcus aureus isolated from milk and milk products in Nasarawa State, Nigeria. A total of 180 samples comprising of fresh raw milk, bulk milk, Nono, and Kindirmo were collected over a period of 6 months. Standard microbiological procedures were employed in the isolation, identification, characterization and determination of the antibiogram of S. aureus from the milk samples. Characterization was achieved by morphological, biochemical characteristics using conventional methods and Microgen® STAPH-ID kits. The isolates were tested for susceptibility or resistance to a panel of 11 commonly used antibiotics using the agar disc diffusion technique. Out of the 180 milk samples examined, nine (9) S. aureus were isolated giving a prevalence of 5.0%. The occurrence of S. aureus was higher in Nono (12.1%) and Kindirmo (10.6%) than in fresh raw milk (5.9%). The kind of water (well water) used for cleaning utensils at the Nono and Kindirmo selling points was found to be a risk factor associated with the occurrence of S. aureus in the products. All of the isolates were resistant to cefoxitin (100%), ampicillin (100%), and amoxicillin/clavulanic acid (100%). The isolates displayed various rates of resistance to erythromycin (22.2%), sulphamethoxazole/trimethoprim (22.2%), and tetracycline (44.4%). Five (5) antibiotic resistance patterns were recorded among the isolates an indication of different levels of use and misuse of antibiotics in the areas studied. The detection of Staphylococcus aureus in fresh and fermented milk in the areas studied suggests that consumption of dairy products especially those produced using traditional methods, constitute a hazard to consumers. It is recommended that since compliance with basic hygiene requirements is not guaranteed, hazard analysis and critical control points (HACCP) concepts should be seen as a part of an effective total hygiene concept at the selling points. Keywords: Antibiotic susceptibility profile, Milk, Nasarawa State, Nigeria, Prevalence, Staphylococcus aureus

scholarly journals Restriction endonuclease characterization of resistant plasmids in Enterobacteriaceae isolated from children in the Sudan

1989 ◽  
Vol 103 (3) ◽  
pp. 487-496 ◽  
Author(s):  
P. Shears ◽  
G. Suliman ◽  
C. A. Hart
Keyword(s):  
Antimicrobial Resistance ◽  
Restriction Endonuclease ◽  
Restriction Enzymes ◽  
Surveillance Systems ◽  
High Prevalence ◽  
Resistance Patterns ◽  
Reproducible Method ◽  
Restriction Endonuclease Digest

SUMMARYThe investigation of plasmid similarity is an important component in the surveillance of antimicrobial resistance and in the detection of epidemic plasmids. The use of restriction endonucleases in the classification of transferable, multiply-resistant plasmids from faecal Enterobacteriaceae isolated at the Children's Emergency Hospital, Khartoum was investigated. Twenty-four transconjugant plasmids, coding for 11 different resistance patterns, each of molecular weight 62 MDa. were studied using four restriction enzymes;PstI,EcoR I,HindIII andAraII. Fifteen different digest profiles were obtained. Restriction profiles discriminated between plasmids with differing resistance patterns and demonstrated homology of plasmids with common resistance patterns. Restriction endonuclease digest patterns provide a potentially rapid and reproducible method of plasmid classification, that could contribute towards surveillance systems in tropical countries with a high prevalence of antimicrobial resistance.

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