Phenotypic and Genotypic Characterization of Streptomycin-Resistant Multidrug-Resistant Mycobacterium tuberculosis Clinical Isolates in Southern China

2020 ◽  
Vol 26 (7) ◽  
pp. 766-775
Author(s):  
Md Mahmudul Islam ◽  
Yaoju Tan ◽  
H.M. Adnan Hameed ◽  
Chiranjibi Chhotaray ◽  
Zhiyong Liu ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Southern China ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Genotypic Characterization ◽  
Phenotypic And Genotypic Characterization

scholarly journals Phenotypic and Genotypic Characterization of Pyrazinamide Resistance among Multidrug-Resistant Mycobacterium tuberculosis Isolates in Zhejiang, China

2015 ◽  
Vol 59 (3) ◽  
pp. 1690-1695 ◽  
Author(s):  
Qiang Xia ◽  
Li-li Zhao ◽  
Feng Li ◽  
Yu-mei Fan ◽  
Yuan-yuan Chen ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Dna Sequencing ◽  
Multidrug Resistant ◽  
Drug Susceptibility Testing ◽  
Zhejiang Province ◽  
Phenotypic Data ◽  
Genotypic Characterization ◽  
Mdr Tb ◽  
Phenotypic And Genotypic Characterization

ABSTRACTTo explore the phenotypic and genotypic characterization of pyrazinamide (PZA) resistance among multidrug-resistantMycobacterium tuberculosis(MDR-TB) isolates in Zhejiang province, a total of 274 MDR-TB isolates were collected. Drug susceptibility testing and spoligotyping were performed on all clinical isolates. In addition, the mutated features of PZA-resistant loci, includingpncAandrpsA, were also analyzed by DNA sequencing. Our results showed that the prevalence of PZA resistance among MDR-TB strains in Zhejiang province was 43.07% and that PZA resistance was associated with concomitant resistance to streptomycin. The majority of PZA-resistant MDR-TB isolates belonged to the Beijing family. Mutations withinpncA, notrpsA, constituted the primary mechanism of PZA resistance. Among 118 PZA-resistant isolates, 53 different mutations were observed inpncA, and most of them were point mutations. Compared with the phenotypic data, DNA sequencing ofpncAhas sensitivity and specificity of 77.97% and 96.79%, respectively. Analysis ofpncAprovided a robust tool for rapid detection of PZA drug resistance.

scholarly journals Detection of Novel Gene Mutations Associated with Pyrazinamide Resistance in Multidrug-Resistant Mycobacterium tuberculosis Clinical Isolates in Southern China

2020 ◽  
Vol Volume 13 ◽  
pp. 217-227 ◽  
Author(s):  
HM Adnan Hameed ◽  
Yaoju Tan ◽  
Md Mahmudul Islam ◽  
Zhili Lu ◽  
Chiranjibi Chhotaray ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Southern China ◽  
Gene Mutations ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Novel Gene
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