Dissemination of Multidrug-Resistant Shigella flexneri and Shigella sonnei with Class 1, Class 2, and Atypical Class 1 Integrons in China

2019 ◽  
Vol 25 (10) ◽  
pp. 1465-1474 ◽  
Author(s):  
Haiquan Kang ◽  
Lei Wang ◽  
Yun Li ◽  
Yuan Lu ◽  
Wenting Fan ◽  
...  
Keyword(s):  
Shigella Flexneri ◽  
Multidrug Resistant ◽  
Shigella Sonnei ◽  
Class 1 Integrons ◽  
Class 1

Antimicrobial Resistance and Presence of Class 1 Integrons Among Different Serotypes of Salmonella spp. Recovered From Children with Diarrhea in Tehran, Iran

2020 ◽  
Vol 20 (2) ◽  
pp. 160-166
Author(s):  
Seyedeh Hanieh Eshaghi Zadeh ◽  
Hossein Fahimi ◽  
Fatemeh Fardsanei ◽  
Mohammad Mehdi Soltan Dallal
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Multidrug Resistant ◽  
Salmonella Spp ◽  
Class 1 Integron ◽  
Class 1 Integrons ◽  
Food Borne ◽  
Class 1 ◽  
Resistance Patterns ◽  
C 30

Background: Salmonellosis is a major food-borne disease worldwide. The increasing prevalence of antimicrobial resistance among food-borne pathogens such as Salmonella spp. is concerning. Objective: The main objective of this study is to identify class 1 integron genes and to determine antibiotic resistance patterns among Salmonella isolates from children with diarrhea. Methods: A total of 30 Salmonella isolates were recovered from children with diarrhea. The isolates were characterized for antimicrobial susceptibility and screened for the presence of class 1 integron genes (i.e. intI1, sulI1, and qacEΔ1). Results: The most prevalent serotype was Enteritidis 36.7%, followed by Paratyphi C (30%), and Typhimurium (16.7%). The highest rates of antibiotic resistance were obtained for nalidixic acid (53.3%), followed by streptomycin (40%), and tetracycline (36.7%). Regarding class 1 integrons, 36.7%, 26.7%, and 33.3% of the isolates carried intI1, SulI, and qacEΔ1, respectively, most of which (81.8%) were multidrug-resistant (MDR). Statistical analysis revealed that the presence of class 1 integron was significantly associated with resistance to streptomycin and tetracycline (p = 0.042). However, there was no association between class 1 integron and other antibiotics used in this study (p > 0.05). Conclusion: The high frequency of integron class 1 gene in MDR Salmonella strains indicates that these mobile genetic elements are versatile among different Salmonella serotypes, and associated with reduced susceptibility to many antimicrobials.

Three Novel Class 1 Integrons Detected in Multidrug-Resistant Pseudomonas aeruginosa Hospital Strains

2019 ◽  
Vol 34 (1) ◽  
pp. 8-15
Author(s):  
E. I. Astashkin ◽  
A. I. Lev ◽  
O. N. Ershova ◽  
T. S. Novikova ◽  
E. N. Ageeva ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Multidrug Resistant ◽  
Class 1 Integrons ◽  
Class 1

scholarly journals Antimicrobial resistance patterns and prevalence of class 1 and 2 integrons in Shigella flexneri and Shigella sonnei isolated in Uzbekistan

Gut Pathogens ◽  
2010 ◽  
Vol 2 (1) ◽  
pp. 18 ◽  
Author(s):  
Ruslan S Madiyarov ◽  
Amir M Bektemirov ◽  
Gulnara A Ibadova ◽  
Gulnara K Abdukhalilova ◽  
Aybek V Khodiev ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Shigella Flexneri ◽  
Shigella Sonnei ◽  
Class 1 ◽  
Resistance Patterns

Characterization of Antibiotic Resistance in Escherichia coli Isolated from Shrimps and Their Environment

2014 ◽  
Vol 77 (8) ◽  
pp. 1394-1401 ◽  
Author(s):  
KANJANA CHANGKAEW ◽  
FUANGFA UTRARACHKIJ ◽  
KANOKRAT SIRIPANICHGON ◽  
CHIE NAKAJIMA ◽  
ORASA SUTHIENKUL ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Multidrug Resistant ◽  
Drug Resistant ◽  
E Coli ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Resistance Rates ◽  
Drug Resistant Strains ◽  
Shrimp Farms

Antimicrobial resistance in bacteria associated with food and water is a global concern. To survey the risk, 312 Escherichia coli isolates from shrimp farms and markets in Thailand were examined for susceptibility to 10 antimicrobials. The results showed that 17.6% of isolates (55 of 312) were resistant to at least one of the tested drugs, and high resistance rates were observed to tetracycline (14.4%; 45 of 312), ampicillin (8.0%; 25 of 312), and trimethroprim (6.7%; 21 of 312); 29.1% (16 of 55) were multidrug resistant. PCR assay of the tet(A), tet(B), tet(C), tet(D), tet(E), and tet(G) genes detected one or more of these genes in 47 of the 55 resistant isolates. Among these genes, tet(A) (69.1%; 38 of 55) was the most common followed by tet(B) (56.4%; 31 of 55) and tet(C) (3.6%; 2 of 55). The resistant isolates were further investigated for class 1 integrons. Of the 55 resistant isolates, 16 carried class 1 integrons and 7 carried gene cassettes encoding trimethoprim resistance (dfrA12 or dfrA17) and aminoglycosides resistance (aadA2 or aadA5). Two class 1 integrons, In54 (dfrA17-aadA5) and In27 (dfrA12-orfF-aadA2), were found in four and three isolates, respectively. These results indicate a risk of drug-resistant E. coli contamination in shrimp farms and selling places. The occurrence of multidrug-resistant E. coli carrying tet genes and class 1 integrons indicates an urgent need to monitor the emergence of drug-resistant E. coli to control the dissemination of drug-resistant strains and the further spread of resistance genes to other pathogenic bacteria.

scholarly journals Genetic and phenotypic determinants of resistance to antibiotics in Aeromonas spp., strains isolated from pediatric patients

2020 ◽  
Vol 14 (10) ◽  
pp. 1146-1154
Author(s):  
Juan Manuel Bello-López ◽  
Carlos Sanchez- Garibay ◽  
Gabriela Ibáñez-Cervantes ◽  
Gregorio León-García ◽  
Luis Uriel Gonzalez-Avila ◽  
...  
Keyword(s):  
Pediatric Patients ◽  
Multidrug Resistant ◽  
Clinical Samples ◽  
Clinical Microbiology Laboratory ◽  
Tertiary Referral Hospital ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Dihydrofolate Reductases ◽  
Epidemiological Risk ◽  
Intestinal Origin

Introduction: Intestinal and extraintestinal infections by Aeromonas spp., remain controversial, due to the existence of healthy carriers of Aeromonas spp. In children under five years old, the diarrhea of infectious origin constitutes the second cause of mortality and remains a major concern for public health. The aim of this work was to detect the pheno/genotype of β-lactamases and class 1 integrons in Aeromonas spp., strains isolated from pediatric patients in a tertiary referral hospital in Mexico. Methodology: Sixty-six strains of Aeromonas spp., were isolated from clinical samples of pediatric origin and were identified by RFLP-PCR 16S rRNA. Resistance phenotype according to CLSI, genetic and phenotypic detection of extended-spectrum β-lactamases (ESBL) and metallo-b-lactamases (MBL) was performed. Finally, characterization of class 1 integrons was performed. Results: Aeromonas spp., strains of diarrheic origin were more predominant. A wide heterogeneity was detected, where A. caviae was the predominant specie. Second-generation cephalosporins, fluoroquinolones, and nitrofurans had best antimicrobial activity; moreover, antibiotics of the β-lactamic and lincosamides families showed lower inhibitory activity. Phenotypically, prevalences of 4.55% and 3.03% were detected for MBL (intestinal origin) and ESBL (extraintestinal origin), respectively. blaIMIS-cphA and blaTEM-1 genes, and nineteen class 1 integrons carrying two variants of cassettes corresponding to adenylyl transferases (aadA), and dihydrofolate reductases (dfrA). Monogenic array with aadA1 cassette was predominantly. Conclusions: ESBL and class 1 integrons, in Aeromonas collected from pediatric patients, determines a major detection challenge for the clinical microbiology laboratory and represents a remarkable epidemiological risk of nosocomial spread of multidrug-resistant determinants.

A Meta-Analysis of the Prevalence of Class 1 Integron and Correlation with Antibiotic Resistance in Pseudomonas aeruginosa Recovered from Iranian Burn Patients

2019 ◽  
Vol 40 (6) ◽  
pp. 972-978 ◽  
Author(s):  
Siamak Heidarzadeh ◽  
Yasamin Enayati Kaliji ◽  
Reza Pourpaknia ◽  
Alireza Mohammadzadeh ◽  
Mehran Ghazali-Bina ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibiotic Resistance ◽  
Meta Analysis ◽  
Multidrug Resistant ◽  
Burn Patients ◽  
Eligibility Criteria ◽  
Class 1 Integron ◽  
Cross Sectional ◽  
Class 1 Integrons ◽  
Class 1

Abstract The role of integrons has been highlighted in antibiotic resistance among Pseudomonas aeruginosa isolates. Therefore, we here reviewed the prevalence of class 1 integrons and their correlations with antibiotic resistance of P. aeruginosa isolated from Iranian burn patients. This review was conducted according to the guidelines of Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA). Cross-sectional and cohort studies published from January 1, 2000 until December 31, 2018 were enrolled. Meta-analysis was performed by Comprehensive Meta-Analysis (CMA) software using the random effects model, Cochran’s Q, and I2 tests. Publication bias was estimated by Funnel plot and Egger’s linear regression test. Nine out of 819 studies met the eligibility criteria. The overall combined prevalence of class 1 integrons in P. aeruginosa isolates was 69% (95% confidence interval [CI]: 50.5–83%). The highest combined resistance was reported against Cloxacillin (87.7%), followed by Carbenicillin (79.1%) and Ceftriaxone (77.3%). The combined prevalence of multidrug-resistant (MDR) isolates was 79.3% (95% CI: 31.1–97%). Also, a significant correlation was noted between the presence of class 1 integrons and antibiotic resistance in 55.5% of the included studies (P < .05). The results showed high prevalence of class 1 integrons, antibiotic resistance, and MDR strains in P. aeruginosa isolated from Iranian burn patients. Also, most of the included studies showed a significant correlation between the presence of class 1 integrons and antibiotic resistance.

Prevalence of Class 1 Integrons in Commensal Escherichia coli from Pigs and Pig Farmers in Thailand

2007 ◽  
Vol 70 (2) ◽  
pp. 292-299 ◽  
Author(s):  
SOUWALAK PHONGPAICHIT ◽  
SUMALEE LIAMTHONG ◽  
ALAN G. MATHEW ◽  
USA CHETHANOND
Keyword(s):  
Escherichia Coli ◽  
Nalidixic Acid ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Frequent Pattern ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Conserved Genes ◽  
Resistance Patterns ◽  
Amoxicillin Clavulanic Acid

Escherichia coli isolates (n = 617) from fecal samples of healthy and diarrheal pigs, pig farmers, and nonfarmers were analyzed for class 1 integrons. Three hundred ninety-two isolates (63.5%) were positive for class 1 integrons, based on the presence of intI1, with seventy-one of those isolates (11.5%) harboring all three conserved genes (intI1, qacEΔ1, and sul1) known to be associated with class 1 integrons. The presence of integrons was associated with isolate origin. Integrons were more prevalent in isolates from most pig groups than in isolates from pig farmers and nonfarmers. Selected integron-positive and integron-negative isolates were tested for resistance to 16 antimicrobials. All integron-positive swine isolates were multidrug resistant to at least three antimicrobial agents, demonstrating resistance to 14 different antibiotics that included sulphamethoxazole (100%), tetracycline (97.1%), ampicillin (92.8%), streptomycin (89.9%), trimethoprim-sulphamethoxazole (88.1%), nalidixic acid (60.9%), chloramphenicol (58.0%), kanamycin (55.1%), cephalothin (44.9%), gentamicin (39.1%), ciprofloxacin (33.3%), cefoxitin (8.7%), amoxicillin–clavulanic acid (5.8%), and amikacin (2.9%). All isolates were susceptible to ceftiofur and ceftriaxone. Forty-seven resistance patterns were observed among 69 integron-positive swine and swine farmer isolates. The most frequent pattern was tetracycline–ciprofloxacin–gentamicin–nalidixic acid–sulphamethoxazole–trimetho-prim-sulphamethoxazole–kanamycin–ampicillin–streptomycin (10.1%), which was found in diarrheal and healthy pigs. This study shows that integrons and multidrug-resistant commensal bacteria are common and appear to be a significant aspect of microbial communities associated with pigs and humans in southern Thailand.

Identification and characterization of class 1 integrons among multidrug-resistant uropathogenic Escherichia coli strains in Mexico

2022 ◽  
Vol 162 ◽  
pp. 105348
Author(s):  
Edgar González-Villalobos ◽  
Rosa María Ribas-Aparicio ◽  
Laura Belmont-Monroy ◽  
Gerardo Aparicio-Ozores ◽  
H. Ángel Manjarrez-Hernández ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistant ◽  
Uropathogenic Escherichia Coli ◽  
Class 1 Integrons ◽  
Class 1 ◽  
Identification And Characterization
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