scholarly journals Genomic Characterization of Nonclonal mcr-1-Positive Multidrug-Resistant Klebsiella pneumoniae from Clinical Samples in Thailand

2018 ◽  
Vol 24 (4) ◽  
pp. 403-410 ◽  
Author(s):  
Apichai Srijan ◽  
Katie R. Margulieux ◽  
Sirigade Ruekit ◽  
Erik Snesrud ◽  
Rosslyn Maybank ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Multidrug Resistant ◽  
Clinical Samples ◽  
Genomic Characterization

scholarly journals Genomic characterization of multidrug-resistant ESBL-producing Klebsiella pneumoniae isolated from a Ghanaian teaching hospital

2019 ◽  
Vol 85 ◽  
pp. 117-123 ◽  
Author(s):  
Nicholas Agyepong ◽  
Usha Govinden ◽  
Alex Owusu-Ofori ◽  
Daniel Gyamfi Amoako ◽  
Mushal Allam ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Teaching Hospital ◽  
Multidrug Resistant ◽  
Genomic Characterization

scholarly journals Characterization of CTX-M-15-Klebsiella pneumoniae from inpatients and outpatients of a teaching hospital

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 444
Author(s):  
Muzaheed Muzaheed ◽  
Naveed Sattar Shaikh ◽  
Saeed Sattar Shaikh ◽  
Sadananda Acharya ◽  
Shajiya Sarwar Moosa ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Hospital Setting ◽  
Multidrug Resistant ◽  
Influential Factor ◽  
Detection Methods ◽  
Clinical Samples ◽  
Resistant Bacteria ◽  
Drug Resistant ◽  
Drug Resistant Bacteria

Background  The presence of Extended-spectrum β-lactamase (ESBL) positive bacteria in hospital setting is an aggravating influential factor for hospitalized patients, and its consequences may be hazardous. Therefore, there is a need for rapid detection methods for newly emerging drug-resistant bacteria. This study was aimed at the molecular characterization of ESBL-positive Klebsiella pneumoniae isolates recovered from clinical samples.   Methods  A total of 513 K. pneumoniae isolates were obtained from various clinical samples during June 2019 to May 2020. The collected isolates were investigated for antimicrobial susceptibility (antibiogram), and PCR and DNA sequencing were performed to analyse the ESBL genes.   Results  Among the 513 isolates, as many as 359 (69.9%) were ESBL producers and 87.5% were multi-drug resistant, while none had resistance to imipenem. PCR scored 3% blaTEM, 3% blaSHV, and 60% blaCTX-M-15 genes for the tested isolates.   Conclusion  The study showed that CTX-M-15 was the major prevalent ESBL type among the isolates. Additionally, all the isolates were susceptible to carbapenems. Screening and detection of ESBL tests are necessary among all isolates from the enterobacteriaceae family in routine microbiology laboratory to prevent associated nosocomial infections. A larger study is essential to understand molecular epidemiology of ESBL producing organisms to minimize morbidities due to these multidrug resistant organisms.

scholarly journals Molecular Characterization of Carbapenemase Producing Klebsiella Pneumoniae Dominance of OXA-48, KPC, VIM and NDM Producers in Khartoum, Sudan

2017 ◽  
Vol 2 (3) ◽  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Carbapenem Resistance ◽  
Multidrug Resistant ◽  
Clinical Samples ◽  
Medical Laboratories ◽  
Molecular Microbiology ◽  
Diffusion Technique ◽  
Carbapenemase Gene ◽  
First Time

Background and Objectives: Little is known about carbapenemase producing Klebsiella pneumoniae (CPK) in Sudan. This study aimed to determine the prevalence of CPK in a major hospital in Khartoum, Sudan between may 2015 - January 2017 and to characterize the isolates and detect the types of carbapenemase (s) they produced. Materials and Methods: The study was done in the Department of Molecular Microbiology, Faculty of Medical Laboratories Science, Al-Neleen University. All the isolates were obtained from clinical samples of patients treated inside the hospitals. Strains of K. pneumoniae resistant to at least one carbapenem (imipenem or meropenem) by using disc diffusion technique according to the CLSI guidelines were included in this study. Molecular detection of carbapenemase genes was achieved using Real-Time PCR (Sacace Biotechnologie, Italie). Results: A total 96 strains of K. pneumoniae of different non duplicated isolates were obtained from different hospitals. Seventy-two percent (70/96) isolates were positive for carbapenemase genes; 59.4% (57/96) were positive for blaKPC genes, 57.3% (55/96) were positive for blaNDM genes, 37.5% (36/96) were positive for blaVIM genes and 35.4% (34/96) were positive for blaOXA-48 genes. Nineteen isolates possessed four genes (blaKPC, blaNDM, blaVIM and blaOXA-48), fourteen isolates possessed three genes{( blaNDM, blaVIM and blaOXA-48=6), (blaKPC, blaNDM, and blaOXA-48=3), (blaKPC, blaNDM and blaVIM =3), (blaKPC, blaVIM and blaOXA-48=2)}, 27 isolates possessed two genes{( blaKPC and blaNDM =21), (blaKPC, blaOXA-48=2), (blaNDM and blaVIM =3), (blaNDM and blaOXA-48=1)}, 10 isolates possessed only one gene (blaKPC =8, blaOXA-48=1 and blaVIM =1) and the remaining 26 isolates were free from these genes. Conclusion & Recommendation: In Sudan, the most common type of carbapenemase gene multidrug-resistant K. pneumoniae is KPC. Co-production of KPC, VIM, NDM and OXA-48 genes are found in K. pneumoniae. To our knowledge, this study was done for the first time in Sudan. Therefore, it is necessary to determine carbapenem resistance in K. pneumoniae isolates and take essential infection control precautions to avoid spread of this resistance.

scholarly journals Virulent and multidrug‐resistant Klebsiella pneumoniae from clinical samples in Balochistan

2021 ◽  
Author(s):  
Sareeen Fatima ◽  
Faiza Liaqat ◽  
Ali Akbar ◽  
Muhammad Sahfee ◽  
Abdul Samad ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Multidrug Resistant ◽  
Clinical Samples

scholarly journals Lemierre’s syndrome associated with hypervirulent Klebsiella pneumoniae: A case report and genomic characterization of the isolate

IDCases ◽  
2021 ◽  
pp. e01173
Author(s):  
Seung Eun Lee ◽  
Ammara Mushtaq ◽  
Melissa Gitman ◽  
Alberto Paniz-Mondolfi ◽  
Marilyn Chung ◽  
...  
Keyword(s):  
Case Report ◽  
Klebsiella Pneumoniae ◽  
Lemierre’S Syndrome ◽  
Genomic Characterization ◽  
Lemierre's Syndrome

Characterization of multidrug-resistant and virulent Klebsiella pneumoniae strains belonging to the high-risk clonal group 258 (CG258) isolated from inpatients in northeastern Brazil

2021 ◽  
Author(s):  
Rafael Nakamura-Silva ◽  
Mariana Oliveira-Silva ◽  
João Pedro Rueda Furlan ◽  
Eliana Guedes Stehling ◽  
Carlos Eduardo Saraiva Miranda ◽  
...  
Keyword(s):  
High Risk ◽  
Klebsiella Pneumoniae ◽  
Multidrug Resistant ◽  
Northeastern Brazil ◽  
Clonal Group
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