Comparison of Mismatch Amplification Mutation Assay PCR and PCR-Restriction Fragment Length Polymorphism for Detection of Major Mutations ingyrAandparCofEscherichia coliAssociated with Fluoroquinolone Resistance

2019 ◽  
Vol 25 (1) ◽  
pp. 23-31 ◽  
Author(s):  
Kadeeja Jazeela ◽  
Gunimala Chakraborty ◽  
Shruthi Seetharam Shetty ◽  
Anusha Rohit ◽  
Indrani Karunasagar ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Fluoroquinolone Resistance ◽  
Mutation Assay ◽  
Restriction Fragment Length ◽  
Mismatch Amplification Mutation Assay

scholarly journals PCR-Restriction Fragment Length Polymorphism Assay for Detection of gyrA Mutations Associated with Fluoroquinolone Resistance in Campylobacter coli

2004 ◽  
Vol 48 (12) ◽  
pp. 4886-4888 ◽  
Author(s):  
Rodrigo Alonso ◽  
Estibaliz Mateo ◽  
Cecilia Girbau ◽  
Estibaliz Churruca ◽  
Irati Martinez ◽  
...  
Keyword(s):  
Restriction Fragment Length Polymorphism ◽  
Gene Product ◽  
Point Mutation ◽  
Restriction Fragment ◽  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Fluoroquinolone Resistance ◽  
Campylobacter Coli ◽  
Restriction Fragment Length

ABSTRACT A fragment of the gyrA gene was sequenced from 34 isolates of Campylobacter coli, including 23 isolates resistant to ciprofloxacin. All ciprofloxacin-resistant isolates examined by DNA sequencing carried a point mutation at position Thr-86 on the gyrA gene product, involving the replacement of Thr-86 by Ile. A combined PCR-restriction fragment length polymorphism technique using RsaI was developed to detect this mutation.

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