Detection of CTX-M-15-Producing Escherichia coli Isolates of Lineages ST131-B2 and ST167-A in Environmental Samples of a Tunisian Hospital

2016 ◽  
Vol 22 (5) ◽  
pp. 399-403 ◽  
Author(s):  
Raoudha Dziri ◽  
Naouel Klibi ◽  
Carla Andrea Alonso ◽  
Ahlem Jouini ◽  
Leila Ben Said ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Environmental Samples

Fingerprints of resistant Escherichia coli O157:H7 from vegetables and environmental samples

2017 ◽  
Vol 98 (1) ◽  
pp. 80-86
Author(s):  
Grace Onyukwo Abakpa ◽  
Veronica J Umoh ◽  
Sijam Kamaruzaman ◽  
Mark Ibekwe
Keyword(s):  
Escherichia Coli ◽  
Environmental Samples ◽  
Escherichia Coli O157

scholarly journals Occurrence of quinolone-resistant Escherichia coli in environmental samples from a sow pool system in Switzerland

2019 ◽  
Vol 161 (6) ◽  
pp. 387-394 ◽  
Author(s):  
S Von Ah ◽  
R Stephan ◽  
K Zurfluh ◽  
X Sidler ◽  
D Kümmerlen
Keyword(s):  
Escherichia Coli ◽  
Environmental Samples ◽  
Pool System

Potential pitfalls in the quantitative molecular detection ofEscherichia coliO157:H7 in environmental matrices

2006 ◽  
Vol 52 (5) ◽  
pp. 482-488 ◽  
Author(s):  
Rebekka R.E Artz ◽  
Lisa M Avery ◽  
Davey L Jones ◽  
Ken Killham
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Real Time Pcr ◽  
Environmental Samples ◽  
Detection Sensitivity ◽  
Escherichia Coli O157 ◽  
Soil System ◽  
E Coli ◽  
Animal Wastes ◽  
Pcr Assays

The detection sensitivity and potential interference factors of a commonly used assay based on real-time polymerase chain reaction (PCR) for Escherichia coli O157:H7 using eae gene-specific primers were assessed. Animal wastes and soil samples were spiked with known replicate quantities of a nontoxigenic strain of E. coli O157:H7 in a viable or dead state and as unprotected DNA. The detection sensitivity and accuracy of real-time PCR for E. coli O157:H7 in animal wastes and soil is low compared to enrichment culturing. Nonviable cells and unprotected DNA were shown to produce positive results in several of the environmental samples tested, leading to potential overestimates of cell numbers due to prolonged detection of nonviable cells. This demonstrates the necessity for the specific calibration of real-time PCR assays in environmental samples. The accuracy of the eae gene–based detection method was further evaluated over time in a soil system against an activity measurement, using the bioluminescent properties of an E. coli O157:H7 Tn5luxCDABE construct. The detection of significant numbers of viable but nonculturable (VBNC) as well as nonviable and possibly physically protected cells as shown over a period of 90 days further complicates the use of real-time PCR assays for quick diagnostics in environmental samples and infers that enrichment culturing is still required for the final verification of samples found positive by real-time PCR methods.Key words: Escherichia coli O157:H7, real-time PCR, animal waste, soil, VBNC.

Evaluation of Polymerase Chain Reaction for Direct Detection of Escherichia coli Strains in Environmental Samples

2007 ◽  
Vol 2 (2) ◽  
pp. 163-169 ◽  
Author(s):  
Safa A. Sherfi . ◽  
Hamid A. Dirar . ◽  
Badr E. Hago . ◽  
Mohamed E. Ahmed . ◽  
Hassan A. Musa . ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Environmental Samples ◽  
Direct Detection ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Occurrence and Characteristics of Mobile Colistin Resistance (mcr) Gene-Containing Isolates from the Environment: A Review

2020 ◽  
Vol 17 (3) ◽  
pp. 1028 ◽  
Author(s):  
Madubuike Umunna Anyanwu ◽  
Ishmael Festus Jaja ◽  
Obichukwu Chisom Nwobi
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Environmental Samples ◽  
Insertion Sequences ◽  
Environmental Isolates ◽  
Colistin Resistance ◽  
Public Places ◽  
Extensive Drug Resistance ◽  
Class 1 ◽  
Genes Encoding

The emergence and spread of mobile colistin (COL) resistance (mcr) genes jeopardize the efficacy of COL, a last resort antibiotic for treating deadly infections. COL has been used in livestock for decades globally. Bacteria have mobilized mcr genes (mcr-1 to mcr-9). Mcr-gene-containing bacteria (MGCB) have disseminated by horizontal/lateral transfer into diverse ecosystems, including aquatic, soil, botanical, wildlife, animal environment, and public places. The mcr-1, mcr-2, mcr-3, mcr-5, mcr-7, and mcr-8 have been detected in isolates from and/or directly in environmental samples. These genes are harboured by Escherichia coli, Enterobacter, Klebsiella, Proteus, Salmonella, Citrobacter, Pseudomonas, Acinetobacter, Kluyvera, Aeromonas, Providencia, and Raulotella isolates. Different conjugative and non-conjugative plasmids form the backbones for mcr in these isolates, but mcr have also been integrated into the chromosome of some strains. Insertion sequences (IS) (especially ISApl1) located upstream or downstream of mcr, class 1–3 integrons, and transposons are other drivers of mcr in the environment. Genes encoding multi-/extensive-drug resistance and virulence are often co-located with mcr on plasmids in environmental isolates. Transmission of mcr to/among environmental strains is clonally unrestricted. Contact with the mcr-containing reservoirs, consumption of contaminated animal-/plant-based foods or water, international animal-/plant-based food trades and travel, are routes for transmission of MGCB.

scholarly journals Phenotypic and genotypic characteristics of Escherichia coli with non-susceptibility to quinolones isolated from environmental samples on pig farms

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Patrick Kindle ◽  
Katrin Zurfluh ◽  
Magdalena Nüesch-Inderbinen ◽  
Sereina von Ah ◽  
Xaver Sidler ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Environmental Samples ◽  
Pig Farms ◽  
Genotypic Characteristics

VIM and IMP metallo-β-lactamases and other extended-spectrum β-lactamases in Escherichia coli and Klebsiella pneumoniae from environmental samples in a Tunisian hospital

Apmis ◽  
2011 ◽  
Vol 119 (10) ◽  
pp. 725-732 ◽  
Author(s):  
CHEDLY CHOUCHANI ◽  
RIM MARRAKCHI ◽  
LEILA FERCHICHI ◽  
ALLAAEDDIN EL SALABI ◽  
TIMOTHY R. WALSH
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Environmental Samples ◽  
Extended Spectrum
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