Molecular and Epidemiological Characterization of Enterobacterial Multidrug-Resistant Strains in Tlemcen Hospital (Algeria) (2008–2010)

2013 ◽  
Vol 19 (3) ◽  
pp. 185-190 ◽  
Author(s):  
Zaket Baba Ahmed-Kazi Tani ◽  
Dominique Decré ◽  
Nathalie Genel ◽  
Zahia Boucherit-Otmani ◽  
Guillaume Arlet ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Resistant Strains ◽  
Epidemiological Characterization

Characterization of swine isolates of Clostridium difficile in Spain: A potential source of epidemic multidrug resistant strains?

Anaerobe ◽  
2013 ◽  
Vol 22 ◽  
pp. 45-49 ◽  
Author(s):  
Teresa Peláez ◽  
Luis Alcalá ◽  
José L. Blanco ◽  
Sergio Álvarez-Pérez ◽  
Mercedes Marín ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Multidrug Resistant ◽  
Potential Source ◽  
Resistant Strains

scholarly journals Streptococcus suis in Brazil: Genotypic, Virulence, and Resistance Profiling of Strains Isolated from Pigs between 2001 and 2016

Pathogens ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 31 ◽  
Author(s):  
Carlos E. C. Matajira ◽  
Luisa Z. Moreno ◽  
Andre P. Poor ◽  
Vasco T. M. Gomes ◽  
Andressa C. Dalmutt ◽  
...  
Keyword(s):  
Streptococcus Suis ◽  
Multidrug Resistant ◽  
Swine Industry ◽  
Pfge Typing ◽  
Resistance Selection ◽  
Important Challenge ◽  
Resistant Strains ◽  
Resistance Rates ◽  
Isolation Period

Streptococcus suis remains an important challenge for the worldwide swine industry. Considering that Brazil is a major pork producer and exporter, proper monitoring of the pathogen and resistance rates are required. We present here the characterization of Brazilian S. suis strains isolated over a 15 year period by pulsed-field gel electrophoresis (PFGE) typing, capsular, virulence, and antimicrobial resistance profiling. Serotype prevalence revealed a predominance of serotype 2/½ followed by 3, 7, 1/14, 6, 8, 18, 28, and 27; the latter had not yet been reported in Brazil. Resistance profiling enabled the differentiation of nine profiles presenting resistance to three and up to eight antimicrobial classes. Even though an association between the most resistant strains and isolation year starting from 2009 was observed, a high frequency of multidrug-resistant strains isolated from 2001 to 2003 was also detected. This suggests that despite the isolation period, S. suis strains already presented high resistance selection pressure. A slight association of serotype 2/½ with some virulence profiles and PFGE pulsotypes was also identified. Nevertheless, no clonal dispersion or persistency of clones over the analyzed years and herds was detected.

scholarly journals Comprehensive Genome Analysis of Carbapenem-Resistant Strains of Raoultella Species, an Emerging Multidrug-Resistant Bacterium in Hospitals

2019 ◽  
Vol 63 (12) ◽  
Author(s):  
Xiao Yu ◽  
Beiwen Zheng ◽  
Jing Zhang ◽  
Hao Xu ◽  
Tingting Xiao ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Antibiotic Resistance Genes ◽  
Genomic Analysis ◽  
Multidrug Resistant ◽  
Control Measures ◽  
Carbapenem Resistant ◽  
Multidrug Resistant Bacterium ◽  
The World ◽  
Resistant Strains

ABSTRACT We report the characterization of six carbapenem-resistant Raoultella spp. (CRRS) in our hospital and a genomic analysis of 58 publicly available isolates. CRRS isolates are sporadically identified around the world, and different transposons carrying carbapenemases were the resistant mechanisms. Mobile genetic elements play an important role in acquiring antibiotic resistance genes from the hospital. An improved understanding of these transposon and targeted control measures will be very valuable to prevent CRRS dissemination.

scholarly journals Characterization of auto-agglutinating and non-typeable uropathogenic Escherichia coli strains

2019 ◽  
Vol 13 (06) ◽  
pp. 465-472
Author(s):  
Ulises Hernández-Chiñas ◽  
Alejandro Pérez-Ramos ◽  
Laura Belmont-Monroy ◽  
María E Chávez-Berrocal ◽  
Edgar González-Villalobos ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Genes ◽  
Antimicrobial Agents ◽  
Multidrug Resistant ◽  
Uropathogenic Escherichia Coli ◽  
E Coli ◽  
Resistant Strains ◽  
Tract Infections ◽  
Disk Method

Introduction: Uropathogenic Escherichia coli (UPEC) are the main etiological agent of urinary tract infections (UTIs). Association between different serotypes and UTIs is known, however, some strains are incapable to be serotyped. The aim of this work was to study bthe phenotypical and genotypical characteristics of 113 non-typeable (NT) and auto-agglutinating (AA) E. coli strains, isolated from UTIs in children and adults. Methodology: The 113 UPEC strains were analyzed by PCR assays using specific primers to determine their serogroups, fimH, papC, iutA, sat, hlyCA and cnf1, virulence associated genes, and chuA, yjaA and TSPE4.C2 for phylogroup determination. Additionally, the diffusion disk method was performed to evaluate the antimicrobial resistance to 18 antimicrobial agents. Results: Using the PCR assay, 63% (71) of the strains were genotyped showing O25 and O75 as the most common serogroups. The virulence genes fimH (86%) and iutA (74%) were the most prevalent, in relation to the phylogroups the commensal (A and B1) and virulent (B2 and D) showed similar frequencies (P > 0.05). The antimicrobial susceptibility test showed a high percentage (73%) of multidrug-resistant strains. Conclusions: The genotyping allowed identifying the serogroup in many of the strains that could not be typed by traditional serology. The strains carried virulence genes and were multidrug-resistant in both, commensal and virulent phylogroups. Our findings revealed that, in addition to the classical UPEC serogroups, there are pathogenic serogroups not reported yet.

Spread of multidrug-resistant clonal complex 271 of serotype 19FStreptococcus pneumoniaein Beijing, China: characterization of serotype 19F

2013 ◽  
Vol 141 (12) ◽  
pp. 2492-2496 ◽  
Author(s):  
Q. H. LI ◽  
K. H. YAO ◽  
S. J. YU ◽  
X. MA ◽  
M. M. HE ◽  
...  
Keyword(s):  
Clonal Complex ◽  
Respiratory Infections ◽  
Multidrug Resistant ◽  
Acute Respiratory Infections ◽  
Resistant Clone ◽  
Resistant Strains ◽  
Beijing China ◽  
Susceptibility Rate

SUMMARYWe investigated the genetic structure of 120 isolates of serotype 19FStreptococcus pneumoniaefrom Chinese children with acute respiratory infections collected from 1997 to 2006, and 2010. All strains were susceptible to vancomycin and levofloxacin, and only five strains were not susceptible to penicillin. The non-susceptibility rate to cephalosporins increased from 1997 to 2010. Of 119 erythromycin-resistant strains, 60 carried bothermBandmefAgenes. The percentage of clonal complex 271 (CC271) increased from 14·3% in 1997–1998 to 92% in 2010, whereas that of CC983 decreased from 64·3% to 0%. CC271 had a higher non-susceptibility rate toβ-lactam antibiotics than CC983 and other CCs. The increased non-susceptibility rate toβ-lactam antibiotics in serotype 19F pneumococci was found to be associated with the spread of the international resistant clone CC271 presumably caused by antibiotic pressure. Long-term surveys of serotype 19FS. pneumoniaeare required to monitor CC prevalence and trends in antimicrobial resistance.

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