Molecular Typing of Methicillin-ResistantStaphylococcus aureusby Pulsed-Field Gel Electrophoresis: Comparison of Results Obtained in a Multilaboratory Effort Using Identical Protocols and MRSA Strains

2000 ◽  
Vol 6 (3) ◽  
pp. 189-198 ◽  
Author(s):  
MARILYN CHUNG ◽  
HERMÍNIA DE LENCASTRE ◽  
PETER MATTHEWS ◽  
ALEXANDER TOMASZ ◽  
MARTA AIRES DE SOUSA ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Comparison Of Results ◽  
Mrsa Strains

scholarly journals Molecular typing of Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan

1999 ◽  
Vol 122 (2) ◽  
pp. 337-341 ◽  
Author(s):  
M. AKIBA ◽  
T. MASUDA ◽  
T. SAMESHIMA ◽  
K. KATSUDA ◽  
M. NAKAZAWA
Keyword(s):  
Escherichia Coli ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Escherichia Coli O157 ◽  
Outbreak Strain ◽  
Pulsed Field ◽  
E Coli ◽  
Biological Methods ◽  
Pfge Profiles

A total of 77 Escherichia coli O157[ratio ]H7 (H−) isolates from cattle in Japan were investigated by molecular biological methods. Most of these isolates (43 isolates) possessed the stx2 gene, but not stx1. Fifteen bacteriophage types and 50 pulsed-field gel electrophoresis (PFGE) profiles were observed. One isolate was indistinguishable from the human outbreak strain by these methods. This indicates that cattle must be considered as a possible source of human E. coli O157[ratio ]H7 infection in Japan.

scholarly journals Surveillance of severe invasive group-G streptococcal infections and molecular typing of the isolates in Japan

2004 ◽  
Vol 132 (1) ◽  
pp. 145-149 ◽  
Author(s):  
T. IKEBE ◽  
S. MURAYAMA ◽  
K. SAITOH ◽  
S. YAMAI ◽  
R. SUZUKI ◽  
...  
Keyword(s):  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Clonal Expansion ◽  
Pulsed Field Gel Electrophoresis ◽  
Streptococcal Infections ◽  
Streptococcus Dysgalactiae ◽  
Invasive Group ◽  
Pulsed Field ◽  
Number Of Patients ◽  
Dna Profiles

The number of patients with severe invasive group-G streptococcal (Streptococcus dysgalactiae subsp. equisimilis) infections has been increasing in Japan. The emm genotypes and SmaI-digested pulsed-field gel electrophoresis DNA profiles were variable among the strains isolated, suggesting there has not been clonal expansion of a specific subpopulation of strains. However, all strains carried scpA, ska, slo and sag genes, some of which may be involved in the pathogenesis of the disease.

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