Anti-human Folate Receptor αβ Antibody

2015 ◽  
Vol 34 (3) ◽  
pp. 223-223
Keyword(s):  
Folate Receptor ◽  
Human Folate Receptor

scholarly journals Sorting and Function of the Human Folate Receptor Is Independent of the Caveolin Expression in Fisher Rat Thyroid Epithelial Cells

BMB Reports ◽  
2002 ◽  
Vol 35 (4) ◽  
pp. 395-402 ◽  
Author(s):  
Chong-Ho Kim ◽  
Young-Soon Park ◽  
Koong-Nah Chung ◽  
Patrick C. Elwood
Keyword(s):  
Epithelial Cells ◽  
Folate Receptor ◽  
Human Folate Receptor ◽  
Rat Thyroid ◽  
And Function

scholarly journals Molecular Docking, Pharmacokinetic Properties and  Toxicity  Studies of  Novel Folate Conjugated To α-Tocopherol via Peptide Bond

2021 ◽  
Author(s):  
Shams H. Abdel-Hafez ◽  
Adil A. Gobouri ◽  
Anber. F. Mohammed ◽  
Mostafa A. Hussein
Keyword(s):  
Molecular Docking ◽  
Folic Acid ◽  
Anticancer Agents ◽  
Folate Receptor ◽  
Peptide Bond ◽  
Docking Study ◽  
Proton Nuclear Magnetic Resonance ◽  
Molecular Docking Study ◽  
Pharmacokinetic Properties ◽  
Human Folate Receptor

Abstract A novel folate conjugated to α-tocopherol (VAF) via peptide bond was Successfully synthesized by using three components vitamin e (α-tocopherol), chloro acetamide and folic acid under mild and simple conditions. The new synthesized compound was characterized by proton nuclear magnetic resonance 1H NMR, 13H NMR and Fourier transform infrared (FT-IR) analysis. A molecular docking study for targeted compound (VAF ) was performed and showed growth-inhibitory activity towards the human folate receptor alpha (FRα) that may affectively the folate uptake by cancer cells. Further, The predicted pharmacokinetic properties and toxicity of the compound (VAF) and folic acid (F) were determined using a statistical analysis (ADMET). It was suggested that pharmacokinetic properties of compound (VAF) represents a promising drug and used as anticancer agents.

MORAb-003 inhibits folate-driven growth mediated by the human folate receptor alpha

2006 ◽  
Vol 24 (18_suppl) ◽  
pp. 10108-10108
Author(s):  
E. Routhier ◽  
H. Turchin ◽  
R. Patel ◽  
W. Ebel ◽  
P. Sass ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cho Cells ◽  
Cellular Proliferation ◽  
Folate Receptor ◽  
Dependent Manner ◽  
Phase I Clinical Trials ◽  
Folate Receptor Alpha ◽  
Receptor Alpha ◽  
Human Folate Receptor

10108 Background: MORAb-003 is a therapeutic humanized antibody currently in Phase I clinical trials for advanced ovarian cancer, and is directed against the human folate receptor alpha (FRα), an antigen which is highly overexpressed on the surface of the majority of ovarian cancers. Overexpression of FRα has been shown to confer a growth advantage to tumorigenic cells in vitro, under conditions of reduced folate availability. We have previously determined that MORAb-003 elicits robust antibody-dependent cellular cytotoxicity (ADCC) and complement-directed cytotoxicity (CDC) in vitro, and is effective in mouse xenograft models of human ovarian cancer. We now show that MORAb-003 possesses novel, growth-inhibitory functions distinct from its immune effector properties. This activity can be recapitulated, using a cell-based assay to score inhibition of folate-dependent growth. Methods: Chinese Hamster Ovary (CHO) cells were engineered to express surface FRα. The cells were cultured under a low folate (< 20 nM) regimen, then re-fed medium supplemented with various concentrations (0–10 μM) of 5-methyltetrahydrofolate (5-MTF), a folate preferentially internalized by the FRα. Cellular proliferation was measured as a function of added 5-MTF, in the presence or absence of MORAb-003. Results: FRα-expressing CHO cells were found to require approximately 100-fold lower 5-MTF than a matched, non-expressing cell line. This increased proliferation could be reduced in a concentration-dependent manner by MORAb-003. Conclusions: MORAb-003 can antagonize the activity of human FRα, resulting in the loss of growth advantage conferred by overexpression of FRα under conditions which bracket physiological (10–100 nM) folate concentrations. [Table: see text]

Escherichia coli expression, purification, and refolding of human folate receptor α (hFRα) and β (hFRβ)

2018 ◽  
Vol 149 ◽  
pp. 17-22 ◽  
Author(s):  
Roopa Dharmatti ◽  
Hideyuki Miyatake ◽  
Chen Zhang ◽  
Xueli Ren ◽  
Akiko Yumoto ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Folate Receptor ◽  
Escherichia Coli Expression ◽  
Human Folate Receptor

Genomic organization of the human folate receptor genes on chromosome 11q13

Genomics ◽  
1992 ◽  
Vol 14 (2) ◽  
pp. 423-430 ◽  
Author(s):  
Jiannis Ragoussis ◽  
Gabriele Senger ◽  
John Trowsdale ◽  
Ian G. Campbell
Keyword(s):  
Genomic Organization ◽  
Folate Receptor ◽  
Chromosome 11Q13 ◽  
Human Folate Receptor

scholarly journals Characterization of the Human Folate Receptor Alpha Via Novel Antibody-Based Probes

Oncotarget ◽  
2011 ◽  
Vol 2 (12) ◽  
pp. 1227-1243 ◽  
Author(s):  
Daniel J. O’Shannessy ◽  
Elizabeth B. Somers ◽  
Earl Albone ◽  
Xin Cheng ◽  
Young Chul Park ◽  
...  
Keyword(s):  
Folate Receptor ◽  
Folate Receptor Alpha ◽  
Receptor Alpha ◽  
Human Folate Receptor
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