scholarly journals Development and Characterization of Neutralizing Monoclonal Antibodies Against the S1 Subunit Protein of QX-like Avian Infectious Bronchitis Virus Strain Sczy3

2015 ◽  
Vol 34 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Nianli Zou ◽  
Fuyan Wang ◽  
Zhenzhen Duan ◽  
Jing Xia ◽  
Xintian Wen ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Virus Strain ◽  
Infectious Bronchitis Virus ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis Virus Strain ◽  
Infectious Bronchitis ◽  
Subunit Protein

scholarly journals Epitopes on the peplomer protein of infectious bronchitis virus strain M41 as defined by monoclonal antibodies

Virology ◽  
1987 ◽  
Vol 161 (2) ◽  
pp. 511-519 ◽  
Author(s):  
Hubert G.M. Niesters ◽  
Nancy M.C. Bleumink-Pluym ◽  
Albert D.M.E. Osterhaus ◽  
Marian C. Horzinek ◽  
Bernard A.M. Van Der Zeijst
Keyword(s):  
Monoclonal Antibodies ◽  
Virus Strain ◽  
Infectious Bronchitis Virus ◽  
Infectious Bronchitis Virus Strain ◽  
Infectious Bronchitis

scholarly journals PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST THE AVIAN INFECTIOUS BRONCHITIS VIRUS

2017 ◽  
Vol 5 (1) ◽  
pp. 71
Author(s):  
Neida Lucia Conrad ◽  
Marcelo Mendonça ◽  
Marcelle Moura Silveira ◽  
Giseli Ritterbush ◽  
Alessandra D'Avila Da Silva ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
B Lymphocytes ◽  
Infectious Bronchitis Virus ◽  
Poultry Production ◽  
Avian Infectious Bronchitis Virus ◽  
Myeloma Cells ◽  
Infectious Bronchitis ◽  
Commercial Poultry ◽  
The Cost

The use of monoclonal antibodies (MAbs) has contributed to the diagnosis of avian infectious bronchitis (IB), however their use are still limited because the utilized MAbs are imported, increasing the cost of such methodologies. Therefore, given the relevance of the national poultry production as well as the losses caused by the occurrence of IB in commercial poultry flocks, it is important that Brazil can produce biological supplies to use for diagnostic of IB. Thus, the present work reports the production, and characterization of MAbs against the infectious bronchitis virus (IBV). Two groups of three Balb-c female mice were inoculated with inactivated classic or variant IBV for 10 times, once per week. The splenocytes from mouse with higher serum concentration against IBV antigens were harvested and fused with murine Sp2/O-Ag14 myeloma cells. We generated two hybridomas secreting MAbs anti-IBV (MAb 2G4 and 5A11) from the fusion of myeloma cells and B-lymphocytes from a mouse immunized with the variant IBV. Following the characterization by ELISA and Western Blot, it was observed that MAbs recognize the IBV classic and variant types, showing more intense reactions with IBV variant. The MAbs obtained in this study have potential for use in immunodiagnostic for IB.

scholarly journals First Complete Genome Sequence of Currently Circulating Infectious Bronchitis Virus Strain DMV/1639 of the GI-17 Lineage

2019 ◽  
Vol 8 (34) ◽  
Author(s):  
Iryna V. Goraichuk ◽  
Arun B. Kulkarni ◽  
Dawn Williams-Coplin ◽  
David L. Suarez ◽  
Claudio L. Afonso
Keyword(s):  
Genome Sequence ◽  
Virus Strain ◽  
Infectious Bronchitis Virus ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Broiler Chickens ◽  
Economic Losses ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis Virus Strain ◽  
Infectious Bronchitis

Avian infectious bronchitis virus is the causative agent of a highly contagious disease that results in severe economic losses to the poultry industry worldwide. Here, we report the first coding-complete genome sequence of strain DMV/1639 of the GI-17 lineage, isolated from broiler chickens in Georgia in 2019.

Sign in / Sign up

Export Citation Format

Share Document