Monoclonal Antibody Against Rabies Virus Proteins by Suckling Mouse Brain Antigen

2014 ◽  
Vol 33 (4) ◽  
pp. 299-299
Keyword(s):  
Monoclonal Antibody ◽  
Rabies Virus ◽  
Mouse Brain ◽  
Suckling Mouse ◽  
Brain Antigen ◽  
Virus Proteins

scholarly journals Gel diffusion test in feline infectious peritonitis with infected suckling mouse brain antigen.

1982 ◽  
Vol 44 (4) ◽  
pp. 695-697
Author(s):  
Hiroyuki NAKAYAMA ◽  
Toshiharu HAYASHI ◽  
Yoshinori WATABE ◽  
Toshihiko YANAGISAWA ◽  
Kosaku FUJIWARA
Keyword(s):  
Mouse Brain ◽  
Suckling Mouse ◽  
Diffusion Test ◽  
Feline Infectious Peritonitis ◽  
Brain Antigen ◽  
Gel Diffusion

scholarly journals AN ELISA SUITABLE FOR THE DETECTION OF RABIES VIRUS ANTIBODIES IN SERUM SAMPLES FROM HUMAN VACCINATED WITH EITHER CELL-CULTURE VACCINE OR SUCKLING-MOUSE-BRAIN VACCINE

1999 ◽  
Vol 41 (1) ◽  
pp. 39-43 ◽  
Author(s):  
Adriana Souza de Toledo PIZA ◽  
Jair Lício Ferreira SANTOS ◽  
Luciana Botelho CHAVES ◽  
Carlos Roberto ZANETTI
Keyword(s):  
Cell Culture ◽  
Rabies Virus ◽  
Mouse Brain ◽  
Neutralization Test ◽  
Protein A ◽  
Staphylococcal Protein A ◽  
Suckling Mouse ◽  
Serum Samples ◽  
Igg Antibody ◽  
Human Sera

An indirect ELISA for determination of post-vaccination rabies antibody was applied. Purified rabies virus was used as antigen to coat plates, and staphylococcal protein A linked with horseradish peroxidase was used for detecting IgG antibody in human sera. Sera from humans, vaccinated with cell-culture vaccine or suckling-mouse-brain vaccine, were examined. ELISA results were compared to those obtained from the virus neutralization test. The mean and standard deviation of OD were determined for 126 negative sera (pre-vaccination) and for 73 sera from vaccinated persons showing antibody titers lower than 0.5 IU/ml. Results were defined as ELISA -positive, -negative or -doubtful. Establishment of a doubtful region reduced the number of sera otherwise classified as positive (false-positive sera). In this way, the sensitivity, specificity and agreement values were respectively 87.5%, 92.4% and 88.5%. No significant differences were observed in these values when the group vaccinated with cell-culture vaccine and the group vaccinated with suckling-mouse-brain vaccine were compared. It was shown that much of the disagreement between the values obtained by neutralization test and ELISA occurred in sera obtained at the beginning of the immunization process, and was probably due to the presence of IgM in the serum samples, detected only by the former test. This ELISA method can be used as a screening test in rabies laboratories regardless of the kind of vaccine used for immunization.

La Crosse virus soluble cell culture antigen

1977 ◽  
Vol 6 (6) ◽  
pp. 618-626
Author(s):  
H S Lindsey ◽  
R A Klimas ◽  
J F Obijeski
Keyword(s):  
Mouse Brain ◽  
Nucleocapsid Protein ◽  
Complement Fixation ◽  
Polyacrylamide Gel Electrophoresis ◽  
Soluble Antigen ◽  
La Crosse Virus ◽  
Suckling Mouse ◽  
Soluble Cell ◽  
Brain Antigen ◽  
Antigen Protein

A virus-free soluble antigen, obtained by ammonium sulfate precipitation of the supernatant fluids of La Crosse virus-infected BHK-21 cell cultures, was more reactive and more specific than infected suckling mouse brain antigen when compared by immunodiffusion and counterelectrophoresis tests. By complement fixation tests, the antigen was cross-reactive with heterologous California group arbovirus hyperimmune mouse ascitic fluids, but to a lesser degree than was the standard sucrose-acetone-extracted infected suckling mouse brain antigen. The major virion nucleocapsid protein of La Crosse virus was found by polyacrylamide gel electrophoresis to be the soluble antigen protein responsible for precipitation in immunodiffusion and counterelectrophoresis tests.

scholarly journals Proteomics analysis of suckling mouse brain infected with attenuated rabies virus strain SRV9

2019 ◽  
Vol 63 (04) ◽  
pp. 423-432
Author(s):  
W. YAN ◽  
R. XIANG ◽  
J. CHEN ◽  
C. HUANG ◽  
Z.-G. YUAN ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Mouse Brain ◽  
Virus Strain ◽  
Suckling Mouse ◽  
Proteomics Analysis ◽  
Rabies Virus Strain

Fatty acylation of rabies virus proteins

Virology ◽  
1991 ◽  
Vol 184 (1) ◽  
pp. 441-444 ◽  
Author(s):  
Yves Gaudin ◽  
Christine Tuffereau ◽  
Abdenour Benmansour ◽  
Anne Flamand
Keyword(s):  
Rabies Virus ◽  
Fatty Acylation ◽  
Virus Proteins

scholarly journals Infection with street strain rabies virus induces modulation of the microRNA profile of the mouse brain

2012 ◽  
Vol 9 (1) ◽  
pp. 159 ◽  
Author(s):  
Pingsen Zhao ◽  
Lili Zhao ◽  
Kun Zhang ◽  
Hao Feng ◽  
Hualei Wang ◽  
...  
Keyword(s):  
Rabies Virus ◽  
Mouse Brain ◽  
Microrna Profile
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