Hardness Sensed by Skin Palpation in Legs with Lymphedema Is Predominantly Correlated with Dermal Thickening

2021 ◽  
Author(s):  
Kotaro Suehiro ◽  
Yukie Mizumoto ◽  
Noriyasu Morikage ◽  
Takasuke Harada ◽  
Makoto Samura ◽  
...  
Keyword(s):  
Dermal Thickening

Exosomes From ADSCs Attenuate Bleomycin-Induced Skin Fibrosis And Oxidative Stress In Scleroderma Via Circ-Zfyve9 Delivery

2021 ◽  
Author(s):  
Zhidong Zhu ◽  
Hui Tang ◽  
Yiqi Zhu ◽  
Hao Wang ◽  
Yanyun Shen
Keyword(s):  
Oxidative Stress ◽  
High Throughput Sequencing ◽  
Skin Fibrosis ◽  
Circular Rnas ◽  
Adipose Derived Stem Cells ◽  
Hypoxic Conditions ◽  
Study Results ◽  
Collagen Accumulation ◽  
And Oxidative Stress ◽  
Dermal Thickening

Abstract Background: Systemic sclerosis (SSc) is autoimmune trait affecting several organs, which is identified by thickening of dermis and connective tissue affected by collagen accumulation, as well as vascular injuries inducing hypoxia. Methods: In this investigation, adipose-derived stem cells (ADSCs) were separated from the ADSC exosomes and a bleomycin-induced SSc mouse model was constructed. We employed high-throughput sequencing to study abnormal expression of circular RNAs (circRNAs) in SSc skin tissues with or without ADSC exosome treatment. The regulatory mechanism and targets were studied using bioinformatics analysis, luciferase reporting analysis, angiogenic differentiation experiments, and RT-qPCR detection. Results: ADSC exosome treatment prevented dermal thickening and fibrosis in bleomycin-induced scleroderma. In addition, circ-Zfyve9 was demonstrated to have an important function in ADSC exosome-mediated skin tissue protection. GPX4 and miR-135 were shown to be circ-Zfyve9 downstream targets. Overexpressing miR-135 or downregulating GPX4 reversed circ-Zfyve9 promotion effects rupon angiopoiesis by promoting lipidosome ROS in EPCs under hypoxic conditions. Overexpressing miR-135 or downregulating GPX4 reversed the circ-Zfyve9 inhibition effect on fibrosis in myofibroblasts under hypoxic conditions. Overexpressing circ-Zfyve9 increased the therapeutic effect of ADSC exosomes. Conclusions: Taken together, the present study results show that the exosomes from ADSCs attenuate bleomycin-induced skin fibrosis and oxidative stress in scleroderma via circ-Zfyve9 delivery.

Opportunistic Mycobacterial Granuloma in a Cat Associated with a Member of the Mycobacterium Terrae Complex

2003 ◽  
Vol 5 (1) ◽  
pp. 37-41 ◽  
Author(s):  
SM Henderson ◽  
J Baker ◽  
R Williams ◽  
DA Gunn-Moore
Keyword(s):  
Systemic Involvement ◽  
Acid Fast Bacilli ◽  
Dermal Thickening

An 18-month-old domestic short-haired neutered male cat presented with a nodular dermal thickening on a digit. Biopsy demonstrated pyogranulomatous inflammation with moderately frequent acid-fast bacilli. A member of the Mycobacterium terrae complex was isolated. There was no evidence of systemic involvement. Treatment was initiated with enrofloxacin, rifampicin and clarithromycin. After 2 months there was no longer any clinically apparent dermal thickening. Treatment was continued for a further 3 months using enrofloxacin and rifampicin.

Presence of galectin-1 in the epidermal and dermal thickening of keloid tissues

2017 ◽  
Vol 1 (1) ◽  
pp. 38 ◽  
Author(s):  
Enrique Arciniegas ◽  
Luz Carrillo ◽  
Hector Rojas ◽  
Richard Ramirez ◽  
Idalina Martinez ◽  
...  
Keyword(s):  
Dermal Thickening

Galectin-1 and Galectin-3 and Their Potential Binding Partners in the Dermal Thickening of Keloid Tissues

2019 ◽  
Vol 41 (3) ◽  
pp. 193-204 ◽  
Author(s):  
Enrique Arciniegas ◽  
Luz Marina Carrillo ◽  
Héctor Rojas ◽  
Richard Ramírez ◽  
Marina Chopite
Keyword(s):  
Binding Partners ◽  
Potential Binding ◽  
Galectin 3 ◽  
Dermal Thickening

scholarly journals Inhibition of sumoylation prevents experimental fibrosis

2012 ◽  
Vol 71 (11) ◽  
pp. 1904-1908 ◽  
Author(s):  
Aisa Khodzhigorova ◽  
Alfiya Distler ◽  
Veronika Lang ◽  
Clara Dees ◽  
Holm Schneider ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Type I ◽  
Myofibroblast Differentiation ◽  
Preclinical Models ◽  
Dermal Fibrosis ◽  
Fibrotic Diseases ◽  
Dermal Thickening ◽  
Novel Therapeutic ◽  
Constitutively Active

ObjectivesFibrosis is a predominant cause of death in systemic sclerosis (SSc). First epigenetic modifications have recently been shown to contribute to activation of SSc fibroblasts. Here, we investigated inhibition of sumoylation as a novel antifibrotic approach.MethodsSumoylation was inhibited by siRNA-mediated knockdown of the Small Ubiquitin-like MOdifiers (SUMO) E2-conjugating enzyme Ubc9, which is essential for sumoylation. The effects of knockdown of Ubc9 were analysed in bleomycin-induced dermal fibrosis, and in the model of fibrosis induced by overexpression of a constitutively active TGF-beta receptor type I (TBR). SUMO-1 and phosphorylated Smad3 were detected by immunohistochemistry.ResultsIncreased staining for SUMO-1 was detected in patients with SSc and in experimental fibrosis. Inhibition of sumoylation exerted potent antifibrotic effects and prevented dermal thickening, myofibroblast differentiation and accumulation of collagen induced by bleomycin, or by overexpression of constitutively active TBR. Moreover, knockdown of Ubc9 reduced the accumulation of phosphorylated Smad3 in experimental fibrosis indicating that inhibition of sumoylation may normalise canonical TGF-β signalling in vivo.ConclusionsWe demonstrate that inhibition of sumoylation reduces canonical TGF-β signalling and prevents experimental fibrosis in different preclinical models. These data provide first evidence that targeting of aberrant sumoylation may be a novel therapeutic approach for fibrotic diseases.

scholarly journals Inhibition of hedgehog signalling prevents experimental fibrosis and induces regression of established fibrosis

2012 ◽  
Vol 71 (5) ◽  
pp. 785-789 ◽  
Author(s):  
Angelika Horn ◽  
Trayana Kireva ◽  
Katrin Palumbo-Zerr ◽  
Clara Dees ◽  
Michal Tomcik ◽  
...  
Keyword(s):  
Small Interfering Rna ◽  
Target Genes ◽  
Hedgehog Signalling ◽  
Dermal Fibrosis ◽  
Tight Skin ◽  
Gli Transcription Factors ◽  
Interfering Rna ◽  
Therapeutic Settings ◽  
Dermal Thickening ◽  
Activation Status

ObjectivesTissue fibrosis is a leading cause of death in patients with systemic sclerosis (SSc). Effective antifibrotic treatments are not available. Here, the authors investigated inhibition of hedgehog signalling by targeting Smoothened (Smo) as a novel antifibrotic approach.MethodsThe activation status of the hedgehog pathway was assessed by immunohistochemistry for Gli transcription factors and by quantification of hedgehog target genes. Hedgehog signalling was inhibited by the selective inhibitor LDE223 and by small interfering RNA against Smo in the models of bleomycin-induced dermal fibrosis and in tight-skin-1 mice.ResultsHedgehog signalling is activated in SSc and in murine models of SSc. Inhibition of Smo either by LDE223 or by small interfering RNA prevented dermal thickening, myofibroblast differentiation and accumulation of collagen upon challenge with bleomycin. Targeting Smo also exerted potent antifibrotic effects in tight-skin-1 mice and did prevent progression of fibrosis and induced regression of pre-established fibrosis.ConclusionsInhibition of hedgehog signalling exerted potent antifibrotic effects in preclinical models of SSc in both preventive and therapeutic settings. These findings might have direct translational implications because inhibitors of Smo are already available and yielded promising results in initial clinical trials.

scholarly journals Separation of retinoid-induced epidermal and dermal thickening from skin irritation

2003 ◽  
Vol 295 (6) ◽  
pp. 255-262 ◽  
Author(s):  
James Varani ◽  
Helene Fligiel ◽  
Jian Zhang ◽  
Muhammad Nadeem Aslam ◽  
Yi Lu ◽  
...  
Keyword(s):  
Skin Irritation ◽  
Dermal Thickening

scholarly journals Microneedle Radiofrequency Combined with Human Fibroblast Conditioned Media for Acne Scars and Skin Rejuvenation

2021 ◽  
Vol 6 (2) ◽  
pp. 1-5
Author(s):  
Tanihaha Edwin ◽  
Keyword(s):  
Acne Vulgaris ◽  
Skin Aging ◽  
Radiofrequency Energy ◽  
Acne Scar ◽  
Skin Texture ◽  
Acne Scars ◽  
Acne Scarring ◽  
Good Improvement ◽  
Grade 3 ◽  
Dermal Thickening

Background: Acne vulgaris is one of the most common dermatology problems in the world. Acne scar can cause stress for sufferers. Microneedle Radiofrequency (MR) clinically has an effectiveness treating acne, acne scars and skin aging. MR acting as electrodes and directly deliver radiofrequency energy deep into the skin to induce new collagen production, dermal thickening and re epitelization. Cases: Seventeen patients (12 acne scar and 5 aging patients, 25-50 years old, Fitzpatrick skin type III-IV) with acne scars and aging skin who received three sessions of microneedle radiofrequency treatment for 2 months at 3 weeks intervalper session. Discussion: We use Goodman and Baron’s Global Acne Scarring System to assess the improvement of acne scars. It showed that by qualitative grading system from 12 patients (grade 3 and grade 4 acne scars), 58% patients showed 2 grade improvement, 33% showed1 grade improvement and 8.3% showed 3 grade improvement. Quantitative assessment showed that 58,3% of the patients had moderate improvement, 25% had good improvement and 16,6% had very good improvement. Global Aesthetic Improvement Scale (GAIS) are used to assess the improvement in skin texture, wrinkles graded by physician. It showed 60 % patients has 51 to 75% improvement and 40% patients has 26 to 50% improvement.

High frequency ultrasound measurement of digital dermal thickness in systemic sclerosis

2009 ◽  
Vol 69 (6) ◽  
pp. 1140-1143 ◽  
Author(s):  
Olga Kaloudi ◽  
Francesca Bandinelli ◽  
Emilio Filippucci ◽  
Maria Letizia Conforti ◽  
Irene Miniati ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
High Frequency ◽  
Interobserver Variability ◽  
Ultrasound Measurement ◽  
Skin Thickness ◽  
Intraobserver Variability ◽  
High Frequency Ultrasound ◽  
Dermal Thickness ◽  
Dermal Thickening ◽  
Frequency Ultrasound

BackgroundCurrently, assessment of dermal thickness in systemic sclerosis (SSc) is performed by palpation and assessment using the modified Rodnan skin score (mRSS).ObjectiveTo verify whether high frequency ultrasound (US) may be a reliable and a reproducible method to measure digital dermal thickness.MethodsIn 70 patients with SSc, skin thickness was evaluated with US by 2 observers at 2 different sites on the second digit of the dominant limb to determine the interobserver variability. Patients and controls were examined twice by the first observer for intraobserver variability. Patients were divided into three subgroups according to the phase of the disease (oedematous, fibrotic or atrophic).ResultsAt both examined areas, US showed a significant dermal thickening (p<0.001) in the whole group of patients with SSc. A low intraobserver and interobserver variability was found. A highly significant correlation between the global mRSS and the local dermal thickness at the two examined sites (p=0.032, p=0.021) was detected. Skin thickness was significantly higher in the oedematous than in the fibrotic group (p<0.001) and significantly higher in the fibrotic and the oedematous group (p<0.001) than in the atrophic group (p<0.002).ConclusionsUS is a reliable tool giving reproducible results, and is able to detect digital dermal thickening in SSc.

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