Antioxidant, Antidiabetic, and Antihypertensive Properties of Echinacea purpurea Flower Extract and Caffeic Acid Derivatives Using In Vitro Models

2017 ◽  
Vol 20 (2) ◽  
pp. 171-179 ◽  
Author(s):  
Shiow-Ying Chiou ◽  
Jih-Min Sung ◽  
Po-Wei Huang ◽  
Sheng-Dun Lin
Keyword(s):  
Caffeic Acid ◽  
Echinacea Purpurea ◽  
In Vitro Models ◽  
Caffeic Acid Derivatives ◽  
Flower Extract ◽  
Antihypertensive Properties

scholarly journals Increasing medicinal and phytochemical compounds of coneflower (Echinacea purpurea L.) as affected by NO3−/NH4+ ratio and perlite particle size in hydroponics

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Fatemeh Ahmadi ◽  
Abbas Samadi ◽  
Ebrahim Sepehr ◽  
Amir Rahimi ◽  
Sergey Shabala
Keyword(s):  
Particle Size ◽  
Caffeic Acid ◽  
Culture Media ◽  
Chemical Compounds ◽  
Echinacea Purpurea ◽  
Hydroponic Culture ◽  
Peat Moss ◽  
Root Extract ◽  
Caffeic Acid Derivatives ◽  
Phytochemical Compounds

AbstractMedicinal plants are considered as one of the most important sources of chemical compounds, so preparing a suitable culture media for medicinal plant growth is a critical factor. The present study is aimed to improve the caffeic acid derivatives and alkylamides percentages of Echinacea purpurea root extract in hydroponic culture media with different perlite particle size and NO3−/NH4+ ratios. Perlite particle size in the growing media was varied as very coarse perlite (more than 2 mm), coarse perlite (1.5–2 mm), medium perlite (1–1.5 mm), fine perlite (0.5–1 mm), and very fine perlite (less than 0.5 mm) in different ratios to peat moss (including pure perlite, 50:50 v/v, 30:70 v/v, and pure peat moss). Two NO3−/NH4+ ratios (90:10 and 70:30) were tested in each growing media. All phytochemical analyses were performed according to standard methods using high performance liquid chromatography (HPLC). It was found that the E. purpurea grown in the medium containing very fine-grade perlite with 50:50 v/v perlite to peat moss ratio had the maximum caffeic acid derivatives, including chicoric acid (17 mg g−1 DW), caftaric acid (6.3 mg g−1 DW), chlorogenic acid (0.93 mg g−1 DW), cynarin (0.84 mg g−1 DW), and echinacoside (0.73 mg g−1 DW), as well as, alkylamides (54.21%). The percentages of these phytochemical compounds increased by decreasing perlite particle size and increasing of NO3−/NH4+ ratio. The major alkylamide in the E. purpurea root extract was dodeca-2E, 4E, 8Z-10 (E/Z)-tetraenoic acid isobutylamide in all treatments, ranging from 31.12 to 54.21% of total dry weight. It can be concluded that optimizing hydroponic culture media and nutrient solution has significant effects on E. purpurea chemical compounds.

Caffeic Acid Derivatives: In Vitro and In Vivo Anti-inflammatory Properties

2004 ◽  
Vol 38 (11) ◽  
pp. 1241-1253 ◽  
Author(s):  
Fernanda M. da Cunha ◽  
Danielle Duma ◽  
Jamil Assreuy ◽  
Fátima C. Buzzi ◽  
Rivaldo Niero ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Caffeic Acid Derivatives ◽  
Anti Inflammatory

scholarly journals Evaluation of the In Vitro Cytotoxic Activity of Caffeic Acid Derivatives and Liposomal Formulation against Pancreatic Cancer Cell Lines

Materials ◽  
2020 ◽  
Vol 13 (24) ◽  
pp. 5813
Author(s):  
Magdalena Zaremba-Czogalla ◽  
Anna Jaromin ◽  
Katarzyna Sidoryk ◽  
Agnieszka Zagórska ◽  
Marcin Cybulski ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Cytotoxic Activity ◽  
Caffeic Acid ◽  
Cell Lines ◽  
Cancer Cell ◽  
Pancreatic Cancer Cell ◽  
Cancer Cell Lines ◽  
Liposomal Formulation ◽  
Caffeic Acid Derivatives

Pancreatic cancer belongs to the most aggressive group of cancers, with very poor prognosis. Therefore, there is an important need to find more potent drugs that could deliver an improved therapeutic approach. In the current study we searched for selective and effective caffeic acid derivatives. For this purpose, we analyzed twelve compounds and evaluated their in vitro cytotoxic activity against two human pancreatic cancer cell lines, along with a control, normal fibroblast cell line, by the classic MTT assay. Six out of twelve tested caffeic acid derivatives showed a desirable effect. To improve the therapeutic efficacy of such active compounds, we developed a formulation where caffeic acid derivative (7) was encapsulated into liposomes composed of soybean phosphatidylcholine and DSPE-PEG2000. Subsequently, we analyzed the properties of this formulation in terms of basic physical parameters (such as size, zeta potential, stability at 4 °C and morphology), hemolytic and cytotoxic activity and cellular uptake. Overall, the liposomal formulation was found to be stable, non-hemolytic and had activity against pancreatic cancer cells (IC50 19.44 µM and 24.3 µM, towards AsPC1 and BxPC3 cells, respectively) with less toxicity against normal fibroblasts. This could represent a promising alternative to currently available treatment options.

scholarly journals Patterns of variation in lipophilic and hydrophilic constituents in flower developmental stages of Echinacea purpurea (L.) Moench cultivated in Slovakia

2009 ◽  
Vol 55 (No. 2) ◽  
pp. 70-73 ◽  
Author(s):  
I. Mistríková ◽  
Š. Vaverková
Keyword(s):  
Developmental Stage ◽  
Caffeic Acid ◽  
Hplc Analysis ◽  
Developmental Stages ◽  
Echinacea Purpurea ◽  
Caffeic Acid Derivatives ◽  
Optimum Yield ◽  
The Third ◽  
Flower Age

The objective of this study was to examine and demonstrate how harvesting age (flower age) contribute to the variations in the quality of <i>Echinacea purpurea</i> (L.) Moench. The effects of different flower developmental stages on caffeic acid derivatives and isobutylamide content are described. These phytochemicals were extracted from fresh plants with 60% ethanol and quantified by the HPLC analysis. The results revealed that the quality of <i>Echinacea</i> is strongly influenced by the flower developmental stages. The highest content of both hydrophilic and lipophilic components in the anthodium of Echinacea plants were found in the third (mature) developmental stage, which is regarded as the optimum one for the harvest to obtain optimum yield levels.

scholarly journals Effect of salicylic acid and yeast extract on caffeic acid derivatives production in Echinacea purpurea L.

2021 ◽  
Vol 20 (78) ◽  
pp. 36-47
Author(s):  
Mohammad Abdoli ◽  
Zhila Mehrpooya ◽  
Mohammad Reza Talebian ◽  
◽  
◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Caffeic Acid ◽  
Yeast Extract ◽  
Echinacea Purpurea ◽  
Caffeic Acid Derivatives

scholarly journals Efficient extraction of caffeic acid derivatives from adventitious roots of Echinacea purpurea

2008 ◽  
Vol 26 (No. 4) ◽  
pp. 254-258 ◽  
Author(s):  
C.H. Wu ◽  
H.N. Murthy ◽  
E.J. Hahn ◽  
H.L Lee ◽  
K.Y. Paek
Keyword(s):  
Immune System ◽  
Herbal Medicine ◽  
Caffeic Acid ◽  
Adventitious Roots ◽  
Total Phenolics ◽  
Echinacea Purpurea ◽  
Food Supplements ◽  
Caffeic Acid Derivatives ◽  
Bioreactor Cultures ◽  
Efficient Extraction

<I>Echinacea</I> species are popular herbal medicine and food supplements for enhancing the immune system. This study was conducted with the aim of developing an efficient heat reflux extraction of caffeic acid derivatives from dried adventitious roots of <I>Echinacea purpurea</I> obtained in bioreactor cultures. Water, methanol (20, 40, 60, 80, and 100%), and ethanol (20, 40, 60, 80, and 100%) were used as solvents for the extraction of caffeic acid derivatives. Another parameter used for the optimisation was the solvent temperature during extraction. The treatment of samples with 60% ethanol at 60°C for 2 h proved to be the most suitable procedure. This treatment was also responsible for the higher yields of total phenolics, flavonoids, and polysaccharides.

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