Anti-Inflammatory Effect of Ethanolic Extract of Sargassum serratifolium in Lipopolysaccharide-Stimulated BV2 Microglial Cells

2016 ◽  
Vol 19 (11) ◽  
pp. 1023-1031 ◽  
Author(s):  
Sun-Ji Oh ◽  
Eun-Ji Joung ◽  
Mi-Sung Kwon ◽  
Bonggi Lee ◽  
Tadanobu Utsuki ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Microglial Cells ◽  
Sargassum Serratifolium ◽  
Anti Inflammatory ◽  
Bv2 Microglial Cells ◽  
Inflammatory Effect

scholarly journals Anti-Inflammatory Effect of Ginsenoside Rg5 in Lipopolysaccharide-Stimulated BV2 Microglial Cells

2013 ◽  
Vol 14 (5) ◽  
pp. 9820-9833 ◽  
Author(s):  
Yu Lee ◽  
Jin-Sun Park ◽  
Ji-Sun Jung ◽  
Dong-Hyun Kim ◽  
Hee-Sun Kim
Keyword(s):  
Microglial Cells ◽  
Ginsenoside Rg5 ◽  
Anti Inflammatory ◽  
Bv2 Microglial Cells ◽  
Inflammatory Effect

Piper sarmentosum Roxb. Root Extracts Confer Neuroprotection by Attenuating Beta Amyloid-Induced Pro-Inflammatory Cytokines Released from Microglial Cells

2019 ◽  
Vol 16 (3) ◽  
pp. 251-260 ◽  
Author(s):  
Elaine Wan Ling Chan ◽  
Emilia Tze Ying Yeo ◽  
Kelly Wang Ling Wong ◽  
Mun Ling See ◽  
Ka Yan Wong ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Mrna Expression ◽  
Inflammatory Mediators ◽  
Beta Amyloid ◽  
Microglial Cells ◽  
Root Extracts ◽  
Tnf Α ◽  
P38α Mapk ◽  
Anti Inflammatory ◽  
Bv2 Microglial Cells

<P>Background: Alzheimer’s disease (AD) is a multifactorial neurodegenerative disorder that eventually leads to severe cognitive impairment. Although the exact etiologies of AD still remain elusive, increasing evidence suggests that neuroinflammation cascades mediated by microglial cells are associated with AD. Piper sarmentosum Roxb. (PS) is a medicinal plant reported to possess various biological properties, including anti-inflammatory, anti-psychotic and anti-oxidant activity. However, little is known about the anti-inflammatory activity of PS roots despite their traditional use to treat inflammatory- mediated ailments. Objective: This study aimed to evaluate the anti-inflammatory and neuroprotective properties of extracts obtained from the roots of PS against beta-amyloid (Aβ)-induced microglial toxicity associated with the production of pro-inflammatory mediators. Method: BV2 microglial cells were treated with hexane (RHXN), dichloromethane (RDCM), ethyl acetate (REA) and methanol (RMEOH) extracts of the roots of PS prior to activation by Aβ. The production and mRNA expression of pro-inflammatory mediators were evaluated by Griess reagent, ELISA kits and RT-qPCR respectively. The phosphorylation status of p38α MAPK was determined via western blot assay. BV2 conditioned medium was used to treat SH-SY5Y neuroblastoma cells and the neuroprotective effect was assessed using MTT assay. Results: PS root extracts, in particular RMEOH significantly attenuated the production and mRNA expression of IL-1β, IL-6 and TNF-α in Aβ-induced BV2 microglial cells. In addition, RHXN, REA and RMEOH extracts significantly reduced nitric oxide (NO) level and the inhibition of NO production was correlated with the total phenolic content of the extracts. Further mechanistic studies suggested that PS root extracts attenuated the production of cytokines by regulating the phosphorylation of p38α MAPK in microglia. Importantly, PS root extracts have protective effects against Aβ-induced indirect neurotoxicity either by inhibiting the production of NO, IL-1β, IL-6, and TNF-α in BV2 cells or by protecting SHSY5Y cells against these inflammatory mediators. Conclusions: These findings provided evidence that PS root extracts confer neuroprotection against Aβ- induced microglial toxicity associated with the production of pro-inflammatory mediators and may be a potential therapeutic agent for inflammation-related neurological conditions including Alzheimer’s disease (AD).</P>

scholarly journals Antioxidant and Anti-inflammatory Activities of N-((3,4-Dihydro-2H-benzo[h]chromene-2-yl)methyl)-4-methoxyaniline in LPS-Induced BV2 Microglial Cells

2015 ◽  
Vol 38 (12) ◽  
pp. 1831-1835 ◽  
Author(s):  
Md. Moniruzzaman ◽  
Gyeongjun Lee ◽  
Shambhunath Bose ◽  
Minho Choi ◽  
Jae-Kyung Jung ◽  
...  
Keyword(s):  
Microglial Cells ◽  
Anti Inflammatory ◽  
Bv2 Microglial Cells

scholarly journals Anti-Inflammatory Activity of Extract Mixture of Annona senegalensis Pers. and Piliostigma thonningii (Schum.)

2020 ◽  
Vol 10 (6-s) ◽  
pp. 103-108
Author(s):  
Komlatsè Togbenou ◽  
Kokou Idoh ◽  
Kossivi Dosseh ◽  
Tchazou Kpatcha ◽  
Amégnona Agbonon
Keyword(s):  
Nitric Oxide ◽  
Ethanolic Extract ◽  
No Production ◽  
Leukocyte Infiltration ◽  
Inflammation Markers ◽  
Asthma Model ◽  
Piliostigma Thonningii ◽  
Anti Inflammatory ◽  
Inflammatory Component ◽  
Inflammatory Effect

Introduction: Annona senegalensis Pers (Annonaceae) and Piliostigma thonningii (Schum.) (Leguminoseae)  are two medicinal plants used, often in combination, in traditional Togolese medicine for the treatment of diseases with an inflammatory component. Objective: The aim of this study is to evaluate the anti-inflammatory effect of the hydro-ethanolic extract (EHEM) of the combination of A. senegalensis and P. thonningii (1: 1, m: m). Methods: The ovalbumin-induced allergic airway asthma model was used. Animals made asthmatic were treated with EHEM at doses of 250 and 500 mg / kg. Inflammation markers including histamine, nitric oxide (NO), vascular leakage, leukocyte infiltration in the airways, and malondialdehyde (MDA), were measured. Results: Compared to the SNT group, EHEM inhibits the infiltration of the airways by leukocytes (850,00 × 103 ± 50 cells / mL vs 1830 × 103 ± 53,85 cells / mL for ST500 mg / kg, P <0,05). It inhibited vascular permeability to Evans Blue (10,40 ± 0,270 μg / mL vs ST500, p<0,05). It inhibited histamine release (13,95 ± 0,937 μg / mL vs 32,78 ± 1,044 μg / mL, p<0,05) and NO production (0,211 ± 0,008 Μm vs 0,315 ± 0,022 μM, p<0,05). It finally inhibited MDA production (14,66 ± 0,533 nM / mL vs 9,014 ± 0,366 nM / mL and 7,149 ± 0,300 nM / mL, p<0,05) in lung tissue. Conclusion: Our results suggest that EHEM inhibits OVA-induced inflammation. These results justify the use of this combination of plants in traditional Togolese medicine. Keywords: Inflammation, malondialdehyde, Annona senegalensis, Piliostigma thonningii.

scholarly journals Anti-inflammatory effects of saponins derived from the roots of Platycodon grandiflorus in lipopolysaccharide-stimulated BV2 microglial cells

2013 ◽  
Vol 31 (6) ◽  
pp. 1357-1366 ◽  
Author(s):  
KYUNG-JUN JANG ◽  
HONG KI KIM ◽  
MIN HO HAN ◽  
YOU NA OH ◽  
HYUN-MIN YOON ◽  
...  
Keyword(s):  
Microglial Cells ◽  
Anti Inflammatory ◽  
Bv2 Microglial Cells

scholarly journals Sesquiterpenoids and Their Anti-Inflammatory Activity: Evaluation of Ainsliaea yunnanensis

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1701
Author(s):  
Jinjie Li ◽  
Xiuting Li ◽  
Xin Wang ◽  
Xiangjian Zhong ◽  
Linlin Ji ◽  
...  
Keyword(s):  
Nlrp3 Inflammasome ◽  
Ethanolic Extract ◽  
The Self ◽  
Inflammatory Activity ◽  
Spectroscopic Methods ◽  
Activity Evaluation ◽  
Caspase 1 ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Four new sesquiterpenoids (1–4) and six known sesquiterpenoids (5–10), were isolated from the EtOAc phase of the ethanolic extract of Ainsliaea yunnanensis. Their structures were established by spectroscopic methods, including 1-D, 2-D NMR and HPLC-MS. All compounds were tested for their anti-inflammatory effect by the inhibition of the activity of NLRP3 inflammasome by blocking the self-slicing of pro-caspase-1, which is induced by nigericin, then the secretion of mature IL-1β, mediated by caspase-1, was suppressed. Unfortunately none of the compounds showed an anti-inflammatory effect.

scholarly journals Anti-Inflammatory Effect of Ethanolic Extract of Carica Papaya Leaves and Indomethacin in Cotton Pellet Induced Granuloma in Animal Model

2019 ◽  
Vol 6 (1) ◽  
pp. 2-5 ◽  
Author(s):  
Afroza Sultana ◽  
Rumana Afroz ◽  
Onayza Yasmeen ◽  
Muqbula Tasrin Aktar ◽  
Md Abdullah Yusuf
Keyword(s):  
Animal Model ◽  
Carica Papaya ◽  
Ethanolic Extract ◽  
Granuloma Formation ◽  
Rat Paw Edema ◽  
Group I ◽  
Cotton Pellet ◽  
Anti Inflammatory ◽  
Group Ii ◽  
Inflammatory Effect

Background: Anti-inflammatory effect can be exerted by Carica papaya. Objective: The purpose of the present study was to see the anti-inflammatory effect of ethanolic extract of Carica papaya leaves and indomethacin in carrageenan induced rat paw edema animal model. Methodology: This was an animal study carried out in the Department of Pharmacology at Dhaka Medical College, Dhaka, Bangladesh during the period from July 2014 to June 2015 for a period of one (01) year. The leaves of Carica Papaya collected from Botanical garden, Mirpur, Dhaka, Bangladesh. The animals were divided into four groups. The animals were divided into four groups. In all the animals granuloma were induced by implantation of autoclaved cotton pellet on the 1st day and they treated as follows: Group I were served as control that was received normal saline. Group II were received ethanolic extract of Carica Papaya leaves. Group III were received ethanolic extract of Carica Papaya leaves. Group IV were received indomethacin. On the 15th day animals were anesthetized, implanted pellets were dissected out, dried at hit air oven and the final weights were measured. Result: The experiment was carried out on 48 Long Evan Norwegian rats. Rats were divided in 4 groups of six animals each. The mean increase of weight of cotton pellet was 48.06±1.02, 24.48±0.36, 22.42±0.56 and 18.02±0.43 in group I, II, III and IV respectively. The inhibition of granuloma formation was 49.06%, 53.35% and 62.51% in group II, III and IV respectively. Conclusion: In conclusion the ethanolic extract of Carica Papaya leaves has effects on cotton pellet induced granuloma formation in experimental rat. Journal of Current and Advance Medical Research 2019;6(1):2-5

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