Comparison of the Total Oxyradical Scavenging Capacity and Oxygen Radical Absorbance Capacity Antioxidant Assays

2007 ◽  
Vol 10 (2) ◽  
pp. 337-344 ◽  
Author(s):  
David P. Tomer ◽  
Lee D. McLeman ◽  
Seiga Ohmine ◽  
Philip M. Scherer ◽  
Byron K. Murray ◽  
...  
Keyword(s):  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity ◽  
Antioxidant Assays ◽  
Scavenging Capacity ◽  
Total Oxyradical Scavenging Capacity

An oxygen radical absorbance capacity-like assay that directly quantifies the antioxidant’s scavenging capacity against AAPH-derived free radicals

2009 ◽  
Vol 386 (2) ◽  
pp. 167-171 ◽  
Author(s):  
Shunji Kohri ◽  
Hirotada Fujii ◽  
Shigeru Oowada ◽  
Nobuyuki Endoh ◽  
Yoshimi Sueishi ◽  
...  
Keyword(s):  
Free Radicals ◽  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity ◽  
Scavenging Capacity

scholarly journals COMPOSITION OF PURIFIED ANTHOCYANIN ISOLATED FROM TEAK AND IT’S IN VITRO ANTIOXIDANT ACTIVITY

2017 ◽  
Vol 9 (9) ◽  
pp. 258 ◽  
Author(s):  
Greeshma Murukan ◽  
Murugan K.
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
In Vitro Culture ◽  
Oxygen Radical ◽  
Sulphonic Acid ◽  
Oxygen Radical Absorbance Capacity ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

Objective: The present study evaluates purification, characterization of anthocyanin from in vitro culture of teak and its antioxidant potential.Methods: Anthocyanin was extracted from in vitro culture, purified by using amber lite XAD column and fractionated by Liquid chromatography mass spectrometry (LC-MS/MS). Various antioxidant assays were carried such as 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), 2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulphonic acid (ABTS), Oxygen radical absorbance capacity (ORAC), Nitric oxide (NO) and Hydrogen peroxide (H2O2).Results: Liquid chromatography mass spectrometry (LC-MS/MS) revealed the major fraction as cyanidin 3-(2-xylosyl-rutinoside) with unknown peaks. The amount of anthocyanin was 15.23 mg/g monomeric anthocyanin. Further, the potential antioxidant capacity of the teak anthocyanin was comparable to common vegetables and fruits. Similarly, high correlations of anthocyanin with antioxidant activity, such as oxygen radical absorbance capacity (ORAC), 2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulphonic acid (ABTS), and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) (r = 0.95, 0.93, and 0.80) were found.Conclusion: The high anthocyanins content and potential antioxidant activity suggests that teak anthocyanin may be applied in the food industry as a good source of natural pigments

scholarly journals Determination of the Antioxidative Activities of Herbs Harvested in Japan by Oxygen Radical Absorbance Capacity Methods

2016 ◽  
Vol 22 (2) ◽  
pp. 301-305
Author(s):  
Manabu Wakagi ◽  
Yuuki Taguchi ◽  
Jun Watanabe ◽  
Tasuku Ogita ◽  
Masao Goto ◽  
...  
Keyword(s):  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity

Role of alkoxyl radicals on the fluorescein-based ORAC (Oxygen Radical Absorbance Capacity) assay

2014 ◽  
Vol 75 ◽  
pp. S38 ◽  
Author(s):  
E Dorta ◽  
E Atala ◽  
A Aspee ◽  
H Speisky ◽  
E Lissi ◽  
...  
Keyword(s):  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity

scholarly journals Chemical Constituents and Antioxidant Activity of Ficus callosa

2011 ◽  
Vol 6 (2) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Phan Van Kiem ◽  
Nguyen Xuan Cuong ◽  
Nguyen Xuan Nhiem ◽  
Dan Thi Thuy Hang ◽  
Nguyen Hoai Nam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Column Chromatography ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Chemical Constituents ◽  
Oxygen Radical ◽  
Oxygen Radical Absorbance Capacity ◽  
Chemical Evidence

One new megastigmane glycoside, ficalloside (1), and eleven known compounds, were isolated from methanol extract of Ficus callosa leaves by repeated column chromatography. Their structures were established on the basis of spectral and chemical evidence. The antioxidant activities of these compounds were measured using the oxygen radical absorbance capacity (ORAC) assay. Compound 8 exhibited potent antioxidant activity of 10.6 μM trolox equivalents at the concentration of 2 μM. At this concentration, compounds 4-7 and 9-12 showed significant antioxidant activity with ranging of 2.1~6.1 μM trolox equivalents.

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