Topical Anti-Inflammatory Activities ofVitis rotundifolia(Muscadine Grape) Extracts in the Tetradecanoylphorbol Acetate Model of Ear Inflammation

2007 ◽  
Vol 10 (4) ◽  
pp. 636-642 ◽  
Author(s):  
Eve E. Bralley ◽  
James L. Hargrove ◽  
Phillip Greenspan ◽  
Diane K. Hartle
Keyword(s):  
Tetradecanoylphorbol Acetate ◽  
Anti Inflammatory ◽  
Muscadine Grape

Anti-inflammatory Effects Of Muscadine Grape Extract In The ApcMin/+ Mouse Model Of Intestinal Tumorigenesis

2011 ◽  
Vol 43 (Suppl 1) ◽  
pp. 49
Author(s):  
Tara E. Tae ◽  
J. Mark Davis ◽  
Jamie L. McClellan ◽  
Jennifer L. Steiner ◽  
Martin D. Carmichael ◽  
...  
Keyword(s):  
Mouse Model ◽  
Intestinal Tumorigenesis ◽  
Grape Extract ◽  
Anti Inflammatory ◽  
Muscadine Grape

Topical Anti-Inflammatory Lignans from Haplophyllum hispanicum Haplophyllum hispanicum

1996 ◽  
Vol 51 (9-10) ◽  
pp. 618-622 ◽  
Author(s):  
J. M. Prieto ◽  
M. C. Recio ◽  
R. M. Giner ◽  
S. Máñez ◽  
A. Massmanian ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Multiple Bond ◽  
Delayed Hypersensitivity ◽  
Spectroscopic Methods ◽  
Heteronuclear Multiple Bond Correlation ◽  
Tetradecanoylphorbol Acetate ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
C Nmr

The present paper reports the results of the methanol extract of Haplophyllum hispanicum Spach on single or repeated local 12-O-tetradecanoylphorbol acetate (TPA) administration and in the oxazolone-induced contact-delayed hypersensitivity mouse ear edemas. Two topical anti-inflammatory aryl naphthalide lignans were isolated from the active fractions of the methanol extract. They were identified by spectroscopic methods, including 13C NMR and heteronuclear multiple bond correlation (HMBC), as diphyllin acetyl apioside and tuberculatin. The former was the most active on acute TPA edema with a ID50 of 0.27 μmol/ear

scholarly journals Jasmine (Jasminum grandiflorum) Flower Extracts Ameliorate Tetradecanoylphorbol Acetate Induced Ear Edema in Mice

2020 ◽  
Vol 15 (4) ◽  
pp. 1934578X2091749
Author(s):  
Dongli Li ◽  
Xiaodan Tang ◽  
Chang Liu ◽  
Huifang Li ◽  
Shuzhen Li ◽  
...  
Keyword(s):  
Petroleum Ether Extract ◽  
Mouse Skin ◽  
Skin Inflammation ◽  
In Vitro Assays ◽  
Published Data ◽  
Ear Edema ◽  
Tetradecanoylphorbol Acetate ◽  
Mouse Ear ◽  
Anti Inflammatory

Published data from in vitro assays support the anti-inflammatory effects of jasmine ( Jasminum grandiflorum Linn.) but limited studies are reported in animal models. Herein, the anti-inflammatory effects of jasmine flower extracts (JFEs) including ethanol extract (JF-EE), petroleum ether extract (JF-PEE), ethyl acetate extract (JF-EAE), and n-butanol extract (JF-BE) were evaluated in a mouse ear edema model. Acute mouse ear skin inflammation was induced by tetradecanoylphorbol acetate (TPA; 125 µg/mL) and then treated with JFEs (100 mg/mL) or dexamethasone (DEX; 6.25 mg/mL; as a positive control). Jasmine flower extracts alleviated ear edema by reducing TPA-increased ear thickness and ear weight by 30.8% to 64.1% and 24.0% to 47.1%, respectively, whereas DEX showed comparable activity (by 71.8% and 49.1%, respectively). Their anti-inflammatory effects were supported by data from the immunohistochemical assays. Jasmine flower extracts reduced the inflammatory cells (from 5.5- to 9.5-fold) and the expressions of inflammation related enzymes including cyclooxygenase-2 and inhibitor of kappa-B kinase (from 1.9- to 2.8-fold and from 7.1- to 11.0-fold, respectively). Findings from this study showed that JFEs were able to ameliorate TPA-induced mouse skin inflammation. However, future studies on the underlying mechanisms of jasmine flower’s anti-inflammatory effects are warranted.

scholarly journals Screening Anti-Inflammatory Effects of Flavanones Solutions

2021 ◽  
Vol 22 (16) ◽  
pp. 8878
Author(s):  
Paola Bustos-Salgado ◽  
Berenice Andrade-Carrera ◽  
Valeri Domínguez-Villegas ◽  
Natalia Díaz-Garrido ◽  
María J. Rodríguez-Lagunas ◽  
...  
Keyword(s):  
Traditional Medicine ◽  
Computational Study ◽  
Kidney Diseases ◽  
Experimental Models ◽  
Biologically Active ◽  
Active Components ◽  
Ear Edema ◽  
Tetradecanoylphorbol Acetate ◽  
Anti Inflammatory

There are a large number of remedies in traditional medicine focused on relieving pain and inflammation. Flavanones have been a potential source in the search for leading compounds and biologically active components, and they have been the focus of much research and development in recent years. Eysenhardtia platycarpa is used in traditional medicine for the treatment of kidney diseases, bladder infections, and diabetes mellitus. Many compounds have been isolated from this plant, such as flavones, flavanones, phenolic compounds, triterpenoid acids, chalcones, sugars, and fatty acids, among others. In this paper, natural flavanone 1 (extracted from Eysenhardtia platycarpa) as lead compound and flavanones 1a–1d as its structural analogues were screened for anti-inflammatory activity using Molinspiration® and PASS Online in a computational study. The hydro alcoholic solutions (FS) of flavanones 1, 1a–1d (FS1, FS1a–FS1d) were also assayed to investigate their in vivo anti-inflammatory cutaneous effect using two experimental models, a rat ear edema induced by arachidonic acid (AA) and a mouse ear edema induced by 12-O-tetradecanoylphorbol acetate (TPA). Histological studies and analysis of pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 were also assessed in AA-inflamed rat ear tissue. The results showed that the flavanone hydro alcoholic solutions (FS) caused edema inhibition in both evaluated models. This study suggests that the evaluated flavanones will be effective when used in the future in skin pathologies with inflammation, with the results showing 1b and 1d to be the best.

Study of the Topical Anti-Inflammatory Activity of Achillea ageratum on Chronic and Acute Inflammation Models

1999 ◽  
Vol 54 (11) ◽  
pp. 937-941 ◽  
Author(s):  
M. A. Gómez ◽  
M. T. Sáenz ◽  
M. D. García ◽  
M. A. Fernández
Keyword(s):  
Neutrophil Migration ◽  
Chloroform Extract ◽  
Tetradecanoylphorbol Acetate ◽  
Chronic Model ◽  
Pure Compounds ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Dose Dependent ◽  
Dose Dependent Effect ◽  
Inflammatory Effect

We have produced a chloroform extract from Achillea which includes stigmasterol and sitosterol. By comparing it with the pure compounds an anti-inflammatory effect (with mouse ears) is assumed. The topical anti-inflammatory effect of the chloroform extract from Achillea ageratum (Asteraceae)and of stigmasterol and β-sitosterol, isolated of this extract has been evaluated, against to 12-0-tetradecanoylphorbol acetate (TPA )-induced mouse ear edema, using simple (acute model) and multiple applications (chronic model) of the phlogistic agent. Myeloperoxydase activity also was studied in the inflamed ears. In the acute model the extract exerted a dose-dependent effect. All the doses assayed (1, 3 and 5 mg/ear) significantly reduced the edema (50% , 66% and 82 % , respectively). The isolated sterols stigmasterol and β-sitosterol (with doses of 0.5 mg/ear) had similar effect as the extract with doses of 1 and 3 mg (59% and 65% respectively). In the chronic model the anti-inflammatory effect generally was a more moderate one. The highest dose of the extract decreased the edema reduction to 26% with the highest dose of the extract applied. With the compounds the effect decreased to 36% with stigmasterol, and 40.6% with β-sitosterol. Myeloperoxydase activity (MPO ) was reduced by the extract and the compounds in the acute model, however, in the chronic edema, the enzyme inhibition was very weak with all treatments even with the standard substance. These results indicate that the chloroform extract of Achillea ageratum and some of the its components stigmasterol and β-sitosterol are more effective as topical anti-inflammatory agents in acute than in the chronic process and their action is markedly influenced by the inhibition of neutrophil migration into inflamed tissue.

scholarly journals Ex Vivo and In Vivo Anti-inflammatory Evaluations of Modulated Flavanones Solutions

Proceedings ◽  
2020 ◽  
Vol 78 (1) ◽  
pp. 23
Author(s):  
Paola Bustos-Salgado ◽  
María J. Rodríguez-Lagunas ◽  
Valeri Domínguez-Villegas ◽  
Berenice Andrade-Carrera ◽  
Ana Calpena-Campmany ◽  
...  
Keyword(s):  
Skin Diseases ◽  
Ex Vivo ◽  
Biological Activities ◽  
Ear Edema ◽  
Tetradecanoylphorbol Acetate ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Mouse Ear Edema ◽  
Inflammatory Effect

Interest has developed in natural molecules due to their clinically proven effects on skin diseases. Flavanones display several biological activities, and recently have been the focus of studies due to their anti-inflammatory effect. To improve their pharmacological profile, four flavanones (A, B, C, and D) were synthesized by structural modification of one natural flavanone 1 (semi-systematic name: (2S)-5,7-dihydroxy-6-prenylflavanone) extracted from Eysenhardtia platycarpa. The hydroalcoholic flavanone solutions (FS) were assayed to investigate their anti-inflammatory effect on two in vivo cutaneous inflammation models. Materials and methods: the topical anti-inflammatory effects of FS were evaluated against models of 12-O-tetradecanoylphorbol acetate (TPA)-induced mouse ear edema and arachidonic acid (AA) in rat ear edema. Results: The vinylogous cyclized derivative (flavanone D) caused edema inhibition in the TPA-induced models with an inhibition of 96.27 ± 1.93%; equally effective and potent in inhibiting the mouse ear edema as indomethacin had been. In addition, the AA-induced increase in ear thickness was reduced the most by the topical application of modulated ether (flavanone B). Conclusions: The in vivo and histology results suggest that flavanones B and D are effective as topical anti-inflammatory agents in inflammatory processes. Thus, this new compound represents a promising agent for the management of skin diseases with an inflammatory component.

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