scholarly journals High-Throughput Quantitative Real-Time Polymerase Chain Reaction Array for Absolute and Relative Quantification of Rhesus Macaque Types I, II, and III Interferon and Their Subtypes

2012 ◽  
Vol 32 (9) ◽  
pp. 407-415 ◽  
Author(s):  
Lynnsie M. Schramm ◽  
Kevin D. Kirschman ◽  
Melissa Heuer ◽  
Aaron A. Chen ◽  
Daniela Verthelyi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Rhesus Macaque ◽  
Real Time ◽  
High Throughput ◽  
Relative Quantification ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Evaluation of a 384–Well Format for High-Throughput Real-Time Reverse Transcription Polymerase Chain Reaction for Avian Influenza Testing

2009 ◽  
Vol 21 (5) ◽  
pp. 679-683 ◽  
Author(s):  
Pamela J. Ferro ◽  
Jason Osterstock ◽  
Bo Norby ◽  
Geoffrey T. Fosgate ◽  
Blanca Lupiani
Keyword(s):  
Polymerase Chain Reaction ◽  
Avian Influenza ◽  
Real Time ◽  
High Throughput ◽  
Reverse Transcription ◽  
Virus Isolation ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Response Planning ◽  
Polymerase Chain

As concerns over the global spread of highly pathogenic avian influenza H5N1 have heightened, more countries are faced with increased surveillance efforts and incident response planning for handling a potential outbreak. The incorporation of molecular techniques in most diagnostic laboratories has enabled fast and efficient testing of many agents of concern, including avian influenza. However, the need for high-throughput testing remains. In this study, the use of a 384–well format for high-throughput real-time reverse transcription polymerase chain reaction (real-time RT-PCR) testing for avian influenza is described. The analytical sensitivity of a real-time RT-PCR assay for avian influenza virus matrix gene with the use of both 96– and 384–well assay formats and serial dilutions of transcribed control RNA were comparable, resulting in similar limits of detection. Of 28 hunter-collected cloacal swabs that were positive by virus isolation, 26 (92.9%) and 27 (96.4%) were positive in the 96– and 384–well assays, respectively; of the 340 hunter-collected swabs that were negative by virus isolation, 45 (13.2%) and 23 (6.8%) were positive in the 96– and 384–well assays, respectively. The data presented herein supports the utility of the 384–well format in the event of an avian influenza outbreak for high-throughput real-time RT-PCR testing.

scholarly journals High-throughput applicable genomic sex typing of chicken by TaqMan real-time quantitative polymerase chain reaction

2010 ◽  
Vol 89 (7) ◽  
pp. 1451-1456 ◽  
Author(s):  
N.F. Rosenthal ◽  
H. Ellis ◽  
K. Shioda ◽  
C. Mahoney ◽  
K.R. Coser ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
High Throughput ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Sex Typing ◽  
Polymerase Chain

A Fully Automated Procedure for the High-Throughput Detection of Avian Influenza Virus by Real-Time Reverse Transcription–Polymerase Chain Reaction

2007 ◽  
Vol 51 (s1) ◽  
pp. 235-241 ◽  
Author(s):  
Montserrat Agüero ◽  
Elena San Miguel ◽  
Azucena Sánchez ◽  
Concepción Gómez-Tejedor ◽  
Miguel Angel Jiménez-Clavero
Keyword(s):  
Polymerase Chain Reaction ◽  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Real Time ◽  
High Throughput ◽  
Reverse Transcription ◽  
Chain Reaction ◽  
High Throughput Detection ◽  
Polymerase Chain

Validation of high-throughput real time polymerase chain reaction assays for simultaneous detection of invasive citrus pathogens

2013 ◽  
Vol 193 (2) ◽  
pp. 478-486 ◽  
Author(s):  
Maria Saponari ◽  
Giuliana Loconsole ◽  
Hui-Hong Liao ◽  
Bo Jiang ◽  
Vito Savino ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
High Throughput ◽  
Simultaneous Detection ◽  
Chain Reaction ◽  
Polymerase Chain

Direct urine polymerase chain reaction for chlamydia and gonorrhoea: a simple means of bringing high-throughput rapid testing to remote settings?

Sexual Health ◽  
2013 ◽  
Vol 10 (4) ◽  
pp. 299 ◽  
Author(s):  
Frashta Rahimi ◽  
Namraj Goire ◽  
Rebecca Guy ◽  
John M. Kaldor ◽  
James Ward ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
High Throughput ◽  
Real Time Pcr ◽  
Point Of Care ◽  
Resistance Mechanisms ◽  
Rapid Screening ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Urine Testing

Background Rapid point-of-care tests (POCTs) for chlamydia (Chlamydia trachomatis) and gonorrhoea (Neisseria gonorrhoeae) have the potential to confer health benefits in certain populations even at moderate sensitivities; however, suitable POCTs for these organisms are currently lacking. Methods: In this study, we investigated the use of direct urine polymerase chain reaction (PCR), with the view of implementing a simplified PCR strategy for high-throughput chlamydia and gonorrhoea screening in remote settings. Briefly, a simple dilution of the urine was performed before adding it directly to a real-time PCR reaction. The method was evaluated using 134 stored urine specimens that had been submitted for chlamydia and gonorrhoea testing and had been tested using a commercial C. trachomatis and N. gonorrhoeae PCR method. These included samples that were PCR-positive for chlamydia (n = 87), gonorrhoea (n = 16) or both (n = 2). Direct urine testing was conducted using previously described in-house real-time PCR methods for C. trachomatis and N. gonorrhoeae as well as for recognised N.gonorrhoeae antimicrobial resistance mechanisms. Results: The overall sensitivities and specificities of the direct urine PCR were 78% and 100% for chlamydia, and 83% and 100% for gonorrhoea. N.gonorrhoeae penicillin and quinolone resistance mechanisms were characterised in 14 of the 18 N. gonorrhoeae-positive samples. Conclusions: The results of this study show that the simplified PCR strategy may be a feasible approach for rapid screening and improving chlamydia and gonorrhoea treatment in remote settings.

High-throughput sequence-based typing strategy for HLA-DRB1 based on real-time polymerase chain reaction

2007 ◽  
Vol 68 (11) ◽  
pp. 915-917 ◽  
Author(s):  
Martin Danzer ◽  
Helene Polin ◽  
Johannes Pröll ◽  
Katja Hofer ◽  
Ingrid Fae ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
High Throughput ◽  
Chain Reaction ◽  
Polymerase Chain
Sign in / Sign up

Export Citation Format

Share Document