Interferon-Mediated Regulation of myc and Ki-ras Oncogene Expression in Long-Term-Treated Murine Viral Transformed Cells

1985 ◽  
Vol 5 (4) ◽  
pp. 613-619 ◽  
Author(s):  
R. EMANOIL-RAVIER ◽  
F. POCHART ◽  
M. CANIVET ◽  
M. GARCETTE ◽  
J. TOBALY-TAPIERO ◽  
...  
Keyword(s):  
Transformed Cells ◽  
Ras Oncogene ◽  
Oncogene Expression

scholarly journals Multilayering and loss of apical polarity in MDCK cells transformed with viral K-ras.

1991 ◽  
Vol 112 (5) ◽  
pp. 873-889 ◽  
Author(s):  
C A Schoenenberger ◽  
A Zuk ◽  
D Kendall ◽  
K S Matlin
Keyword(s):  
Cell Surface ◽  
Mdck Cells ◽  
Discrete Distribution ◽  
Free Cell ◽  
Transformed Cells ◽  
Cell Contact ◽  
Ras Oncogene ◽  
Oncogene Expression ◽  
Transformed Cell Line ◽  
Flux Measurements

The effects of viral Kirsten ras oncogene expression on the polarized phenotype of MDCK cells were investigated. Stable transformed MDCK cell lines expressing the v-K-ras oncogene were generated via infection with a helper-independent retroviral vector construct. When grown on plastic substrata, transformed cells formed continuous monolayers with epithelial-like morphology. However, on permeable filter supports where normal cells form highly polarized monolayers, transformed MDCK cells detached from the substratum and developed multilayers. Morphological analysis of the multilayers revealed that oncogene expression perturbed the polarized organization of MDCK cells such that the transformed cells lacked an apical--basal axis around which the cytoplasm is normally organized. Evidence for selective disruption of apical membrane polarity was provided by immunolocalization of membrane proteins; a normally apical 114-kD protein was randomly distributed on the cell surface in the transformed cell line, whereas normally basolateral proteins remained exclusively localized to areas of cell contact and did not appear on the free cell surface. The discrete distribution of the tight junction-associated ZO-1 protein as well as transepithelial resistance and flux measurements suggested that tight junctions were also assembled. These findings indicate that v-K-ras transformation alters cell-substratum and cell-cell interactions in MDCK cells. Furthermore, v-K-ras expression perturbs apical polarization but does not interfere with the development of a basolateral domain, suggesting that apical and basolateral polarity in epithelial cells may be regulated independently.

Immunohistochemical evaluation of ras oncogene expression in pulmonary and pleural neoplasms

1987 ◽  
Vol 53 (1) ◽  
pp. 146-152 ◽  
Author(s):  
Inchul Lee ◽  
Victor E. Gould ◽  
James A. Radosevich ◽  
Ann Thor ◽  
Yixing Ma ◽  
...  
Keyword(s):  
Ras Oncogene ◽  
Oncogene Expression ◽  
Pleural Neoplasms

Demonstration of myc and ras oncogene expression by hybridization in situ in hydatidiform mole and in the BeWo choriocarcinoma cell line

1986 ◽  
Vol 154 (2) ◽  
pp. 390-393 ◽  
Author(s):  
Saumyendra Sarkar ◽  
Barry M. Kacinski ◽  
Ernest I. Kohorn ◽  
Maria J. Merino ◽  
Darryl Carter ◽  
...  
Keyword(s):  
Cell Line ◽  
Hydatidiform Mole ◽  
Ras Oncogene ◽  
Oncogene Expression ◽  
Hybridization In Situ ◽  
Choriocarcinoma Cell Line

scholarly journals Context-Dependent Impact of RAS Oncogene Expression on Cellular Reprogramming to Pluripotency

2019 ◽  
Vol 12 (5) ◽  
pp. 1099-1112 ◽  
Author(s):  
Alba Ferreirós ◽  
Pablo Pedrosa ◽  
Sabela Da Silva-Álvarez ◽  
Francisco Triana-Martínez ◽  
Jéssica M. Vilas ◽  
...  
Keyword(s):  
Cellular Reprogramming ◽  
Ras Oncogene ◽  
Oncogene Expression ◽  
Context Dependent

Reprogramming of Oncogene Expression in Gingival Mesenchymal Stem Cells Following Long-Term Culture In Vitro

2017 ◽  
Vol 19 (3) ◽  
pp. 159-170 ◽  
Author(s):  
Agnese Gugliandolo ◽  
Thangavelu Soundara Rajan ◽  
Domenico Scionti ◽  
Francesca Diomede ◽  
Placido Bramanti ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Culture In Vitro ◽  
Long Term Culture ◽  
Oncogene Expression

Inhibition of Interferon-γ Antiviral and Antiproliferative Activities by ras Oncogene Expression

1989 ◽  
Vol 81 (13) ◽  
pp. 1014-1020
Author(s):  
M. Gariglio ◽  
G. Gribaudo ◽  
A. Franco ◽  
M. A. Pierotti ◽  
G. Cavallo ◽  
...  
Keyword(s):  
Interferon Γ ◽  
Ras Oncogene ◽  
Oncogene Expression ◽  
Antiproliferative Activities

Ras oncogene expression as a prognostic indicator in rectal adenocarcinoma

1988 ◽  
Vol 45 (1) ◽  
pp. 15-20 ◽  
Author(s):  
Fabrizio Michelassi ◽  
Luca E. Vannucci ◽  
Anthony Montag ◽  
Richard Chappell ◽  
Jennifer Rodgers ◽  
...  
Keyword(s):  
Rectal Adenocarcinoma ◽  
Prognostic Indicator ◽  
Ras Oncogene ◽  
Oncogene Expression

scholarly journals Ras oncogene expression and DNA content in plasma cell dyscrasias: a flow cytofluorimetric study

1990 ◽  
Vol 62 (5) ◽  
pp. 781-785 ◽  
Author(s):  
M Danova ◽  
A Riccardi ◽  
G Ucci ◽  
R Luoni ◽  
M Giordano ◽  
...  
Keyword(s):  
Plasma Cell ◽  
Dna Content ◽  
Ras Oncogene ◽  
Oncogene Expression ◽  
Plasma Cell Dyscrasias
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