Development of an In Vitro Model to Assess Deposition of Aerosol Particles in a Representative Replica of the Rat's Respiratory Tract

2012 ◽  
Vol 25 (3) ◽  
pp. 169-178 ◽  
Author(s):  
Arabe Ahmed ◽  
David Prime ◽  
Patricia K.P. Burnell ◽  
Petra Högger
Keyword(s):  
Respiratory Tract ◽  
In Vitro Model ◽  
Aerosol Particles ◽  
Vitro Model

An Optimized In Vitro Model of the Respiratory Tract Wall to Study Particle Cell Interactions

2006 ◽  
Vol 19 (3) ◽  
pp. 392-405 ◽  
Author(s):  
Fabian Blank ◽  
Barbara M. Rothen-Rutishauser ◽  
Samuel Schurch ◽  
Peter Gehr
Keyword(s):  
Respiratory Tract ◽  
In Vitro Model ◽  
Cell Interactions ◽  
Vitro Model

Fluid and Particle Transport in an In-Vitro Model of an Expanding/Contracting Human Alveolus

2007 ◽  
Author(s):  
Sudhaker Chhabra ◽  
Ajay K. Prasad
Keyword(s):  
Particulate Matter ◽  
Respiratory Tract ◽  
Fluid Mechanics ◽  
Respiratory System ◽  
Particle Transport ◽  
In Vitro Model ◽  
Adverse Health Effects ◽  
Fields Of Study ◽  
Vitro Model

Inhaled particulate matter from the environment can produce adverse health effects on the human respiratory system. Conversely, inhalable therapeutics can be delivered to the respiratory tract to treat local and systemic ailments. Both of these fields of study require the accurate prediction of particle transport and deposition in the lung, particularly in the acinar region. A necessary first step to predict particle trajectories is to characterize the airflow in which the particles are suspended. Only particles smaller than 5 μm reach the acinar region [1], hence it can be assumed that such particles will closely follow the fluid streamlines. The current work focuses on the fluid mechanics of the acinar region of the lung to infer particle transport and deposition.

Development Of A Respiratory Tract 3D Glandular Acinar In Vitro Model

2010 ◽  
Author(s):  
Xiaofang Wu ◽  
Jennifer Peters-Hall ◽  
Maria T. Pena ◽  
Mary C. Rose
Keyword(s):  
Respiratory Tract ◽  
In Vitro Model ◽  
Vitro Model

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

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