Regulation of Differentiation of the Tracheobronchial Epithelium

2000 ◽  
Vol 13 (3) ◽  
pp. 207-218 ◽  
Author(s):  
PAUL NETTESHEIM ◽  
JA SEOK KOO ◽  
THOMAS GRAY
Keyword(s):  
Tracheobronchial Epithelium

Tracheobronchial epithelium of the sheep: III. Carbohydrate histochemical and cytochemical characterization of secretory epithelial cells

1988 ◽  
Vol 221 (1) ◽  
pp. 540-549 ◽  
Author(s):  
Andrew T. Mariassy ◽  
Judith A. St. George ◽  
Susan J. Nishio ◽  
Charles G. Plopper
Keyword(s):  
Epithelial Cells ◽  
Tracheobronchial Epithelium

Epithelial stem cell-mediated development of the human respiratory mucosa in SCID mice

2000 ◽  
Vol 113 (5) ◽  
pp. 767-778 ◽  
Author(s):  
A. Delplanque ◽  
C. Coraux ◽  
R. Tirouvanziam ◽  
I. Khazaal ◽  
E. Puchelle ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Sex Chromosome ◽  
Severe Combined Immunodeficiency ◽  
Scid Mice ◽  
Functional Assay ◽  
Combined Immunodeficiency ◽  
Freezing And Thawing ◽  
Normal Epithelium ◽  
Tracheobronchial Epithelium

We have developed an in vivo assay for progenitor cells of the human tracheobronchial epithelium relying on the transplantation of human prenatal respiratory tissues into severe combined immunodeficiency mice. Engrafted embryonic or fetal open tracheobronchial rudiments are rapidly closed at each end by a neoformed membrane that we named the operculum. After 2–4 weeks, differentiated human respiratory epithelium covers both the native airway matrix and the new operculum. Human epithelial cells dissociated from either emerging embryonic lung primordia or mature xenografts were seeded in host human airway grafts, of which native epithelium had been eliminated by several cycles of freezing and thawing. All grafts seeded with donor epithelial cells and implanted back into SCID mice recovered a surface mucociliary epithelium expressing expected markers and secreting mucus. Spontaneous epithelium regrowth was never observed in control unseeded, denuded grafts. In some experiments, donor epithelial cells and host denuded airway were sex-mismatched and the donor origin of newly formed epithelial structures was confirmed by sex chromosome detection. After two rounds of seeding and reimplantation, a normal epithelium was observed to line the 3rd generation operculum. These observations substantiate a functional assay for human candidate airway epithelium stem cells.

Growth and differentiation factors in tracheobronchial epithelium

1991 ◽  
Vol 260 (6) ◽  
pp. L361-L373 ◽  
Author(s):  
A. M. Jetten
Keyword(s):  
Normal Structure ◽  
Cell Types ◽  
Regulatory Factors ◽  
Biochemical Processes ◽  
Polypeptide Growth Factors ◽  
Tracheobronchial Epithelium ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Population Interactions ◽  
Different Cell Types

The normal tracheobronchial epithelium is continuously renewing itself: cells are lost and replaced by the proliferation and differentiation of stem cells. The proliferation and differentiation of these cells have to be tightly controlled in order to maintain the normal structure of the epithelium. A variety of biological and biochemical processes are involved in controlling the proliferation and differentiation of the tracheobronchial epithelium. Since the trachea and bronchus are comprised of a heterogeneous cell population, interactions between the different cell types are of crucial importance not only in controlling the normal maintenance of this tissue but also in the regulation of repair processes following injury and morphogenesis during lung development. A variety of factors, including several polypeptide growth factors and cytokines, have been identified that regulate positively or negatively the growth and differentiation of tracheobronchial epithelial cells by autocrine or paracrine mechanisms. Retinoids are another group of regulatory factors that appear to play a crucial role in controlling cell proliferation and differentiation in the tracheobronchial epithelium. Recently, many advances have been made in understanding the action of these agents in these cells. Alterations in the balance between growth and differentiation regulatory factors appear to play an important role in several pathophysiological changes such as hyperplasia, fibrosis, and neoplasia.

scholarly journals Chemical carcinogenesis in the tracheobronchial epithelium.

1984 ◽  
Vol 55 ◽  
pp. 77-84 ◽  
Author(s):  
B F Trump ◽  
E M McDowell ◽  
C C Harris
Keyword(s):  
Chemical Carcinogenesis ◽  
Tracheobronchial Epithelium

scholarly journals Intraepithelial airway dendritic cells: a distinct subset of pulmonary dendritic cells obtained by microdissection.

1992 ◽  
Vol 175 (3) ◽  
pp. 797-807 ◽  
Author(s):  
J L Gong ◽  
K M McCarthy ◽  
J Telford ◽  
T Tamatani ◽  
M Miyasaka ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Intercellular Adhesion Molecule ◽  
Immunoperoxidase Staining ◽  
Intercellular Adhesion Molecule 1 ◽  
Ifn Gamma ◽  
Distinct Subset ◽  
Tracheobronchial Epithelium ◽  
And Function

Dendritic cells (DC), in general, and pulmonary DC, in particular, are a heterogeneous population of cells, their phenotype and function being dependent on their anatomic location, their state of activation, and the regulatory effect of locally secreted cytokines. Using a novel microdissection technique, the epithelium from the trachea and entire airway system was harvested, and the contained DC isolated at greater than 90% purity. The phenotype and function of these airway DC (ADC) was compared to DC isolated, at greater than 90% purity, from the parenchyma of the same lung. In contrast to lung DC (LDC), ADC did not express intercellular adhesion molecule 1 (ICAM-1) in situ, the amount of immune associated antigen (Ia) expressed was less (as determined by immunoperoxidase staining and immunopanning), and greater than 50% of ADC displayed Fc receptors (FcR). The majority of LDC were ICAM-1+, less than 5% expressed FcR, and all were intensely Ia+. Airway DC were most numerous in tracheal epithelium, but they were also present in small numbers in the epithelium of the most distal airways. Their numbers increased in all segments of the tracheobronchial epithelium in response to the administration of IFN-gamma. ADC were consistently more effective than LDC in presenting soluble (hen egg lysozyme) and particulate (heat-killed Listeria monocytogenes) antigens to antigen-sensitized T cells. By contrast, LDC were significantly more efficient in stimulating the proliferation of nonsensitized T cells in an autologous mixed leukocyte reaction. These data suggest that in normal animals, intraepithelial DC of airways share many attributes with Langerhans cells of the skin. Interstitial LDC, by contrast, reside in an environment where they may be exposed to a different set of regulatory factors and where they have progressed to a more advanced stage of differentiation than ADC. Both groups of DC are, however, heterogeneous, reflecting the continuous turnover that these cells undergo in the lung.

The Role of Growth Factors in the Regulation of Proliferation of Tracheobronchial Epithelium

CHEST Journal ◽  
1992 ◽  
Vol 101 (3) ◽  
pp. 23S-24S ◽  
Author(s):  
Paul Nettesheim ◽  
Patrice Ferriola ◽  
Ronald Steigerwalt ◽  
Alice Robertson ◽  
Joyce Rundhaug
Keyword(s):  
Growth Factors ◽  
Tracheobronchial Epithelium ◽  
Regulation Of Proliferation
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