Synthesis of Pectin Methylesterase from Aspergillus niger in Submerged Fermentation Using as Citrus Pectin and Orange Peel as Inducers

2018 ◽  
Vol 14 (4) ◽  
pp. 212-221
Author(s):  
Jonaina Pili ◽  
Cindy Elena Bustamante Vargas ◽  
Carolina Elisa Demaman Oro ◽  
Geciane Toniazzo Backes ◽  
Eunice Valduga ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Submerged Fermentation ◽  
Orange Peel ◽  
Pectin Methylesterase ◽  
Citrus Pectin

scholarly journals Production of polygalacturonase by Aspergillus niger BC23 isolated from Irvingia gabonensis (African mango) fruit

2016 ◽  
Vol 70 (6) ◽  
pp. 717-724
Author(s):  
Nwokoro Ogbonnaya ◽  
Eze Chukwuemeka
Keyword(s):  
Enzyme Activity ◽  
Aspergillus Niger ◽  
Enzyme Production ◽  
Carbon Sources ◽  
Orange Peel ◽  
Relative Activity ◽  
Maximum Activity ◽  
Citrus Pectin ◽  
Enzyme Productivity ◽  
Irvingia Gabonensis

Polygalacturonase was produced from Aspergillus niger BC 23 which was isolated from spoiled Irvingia gabonensis fruit. The influence of carbon substrates on enzyme production showed that the medium containing sucrose produced a maximum enzyme yield of 38.5 U/mg protein after 72 h. Enzyme productivity in this medium was much higher than in the medium that contained only citrus pectin as the sole carbon source. Medium containing yeast extract as a nitrogen source caused the production of specific enzyme activity of 31.2 U/mg protein. Results on the effect of metal ions on enzyme activity showed that Ca2+ gave a percent relative activity of 214% in comparison to the native enzyme activity. The enzyme showed maximum activity in slight acid and neutral pH media with optimal activity at pH 4.0. Temperature activity profile of the enzyme showed best activity at a temperature of 35?C. Dried fruit peels were tested for their abilities to support enzyme production in a media devoid of citrus pectin. The best enzyme productivity of 102.3 U/mg protein was achieved after 72 h in the medium containing orange peel and this level was much higher than that achieved when pure carbon sources or citrus pectin alone were used for enzyme production.

scholarly journals SCREENING OF ASPERGILLUS NIGER ISOLATED FROM SOIL FOR PECTINASE PRODUCTION

2020 ◽  
Vol 4 (2) ◽  
pp. 244-249
Author(s):  
Abubakar Madika ◽  
Ulem Asagha Eugene ◽  
Musa Bishir ◽  
Mamunu Abdulkadir Sulaiman ◽  
Ibrahim Mohammed Hussaini
Keyword(s):  
Aspergillus Niger ◽  
Submerged Fermentation ◽  
Botanical Garden ◽  
Culture Technique ◽  
Soil Samples ◽  
Citrus Pectin ◽  
Fermentation Conditions ◽  
Cotton Blue ◽  
Pectinase Activity ◽  
Pectinase Production

This study was conducted to screen for pectinase production by Aspergillus niger isolated from soil samples collected from three different locations within Ahmadu Bello University, Zaria, namely; botanical garden, refuse dump and sheep pen sites. A total of fifteen (15) soil samples were collected from different locations and used for isolation by a cultural method. Isolates suspected to be Aspergillus niger were further identified by microscopic examination of the lactophenol cotton blue stained-preparation and slide culture technique. The isolates were then screened in a pectin-containing medium for their pectinase activity. The isolates were further subjected to pectinase production using citrus pectin as the substrate under submerged fermentation conditions. Seven (7) isolates were confirmed to be Aspergillus niger with percentage occurrence of 60% each from sheep pen (SP) and refuse dumpsites (RD), and 20% from the botanical garden (BG). Aspergillus niger RD3 produced the highest zone of pectin hydrolysis (53 ± 1.1 mm) while isolate RD5 produced the lowest (35 ± 3.1 mm). Under submerged fermentation conditions, Aspergillus niger SP5 had the highest pectinase activity of 2.92 U/mL while isolate RD4 had the lowest pectinase activity of 1.29 U/mL. Aspergillus niger can be readily isolated from various soil types with the highest frequency in soils from sheep pen and refuse dumpsites. All the Aspergillus niger isolates demonstrated the potential for pectinase production. The study reveals the potential of various Aspergillus niger isolates from different soil in the production of pectinase.

Expression and characterization of a pectin methylesterase from Aspergillus niger ZJ5 and its application in fruit processing

2018 ◽  
Vol 126 (6) ◽  
pp. 690-696 ◽  
Author(s):  
Zhiwei Zhang ◽  
Junshuai Dong ◽  
Deqing Zhang ◽  
Jiaojiao Wang ◽  
Xing Qin ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Pectin Methylesterase ◽  
Fruit Processing

Studies on the Biosynthesis of Enzyme Xylanase by Submerged Fermentation from Aspergillus niger GCBMX-45

2002 ◽  
Vol 5 (12) ◽  
pp. 1309-1310 ◽  
Author(s):  
Ikram-ul- Haq ◽  
Amna Ehsan ◽  
Waseem Ahmad Butt ◽  
Sikander Ali
Keyword(s):  
Aspergillus Niger ◽  
Submerged Fermentation

Production of acidic lipase by a mutant of Aspergillus niger NCIM 1207 in submerged fermentation

2004 ◽  
Vol 39 (12) ◽  
pp. 2031-2034 ◽  
Author(s):  
Nutan D. Mahadik ◽  
Kulbhushan B. Bastawde ◽  
Ulka S. Puntambekar ◽  
Jayant M. Khire ◽  
Digambar V. Gokhale
Keyword(s):  
Aspergillus Niger ◽  
Submerged Fermentation ◽  
Acidic Lipase
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