SHORT COMMUNICATION: Ambient gas exchange-enabled stirred culture bottle: A stirred tank reactor alternative to shake flasks for high gas-liquid mass transfer

2010 ◽  
Vol 6 (2) ◽  
pp. 100-103 ◽  
Author(s):  
Duen Gang Mou
Keyword(s):  
Mass Transfer ◽  
Gas Exchange ◽  
Short Communication ◽  
Stirred Tank ◽  
Culture Bottle ◽  
Stirred Tank Reactor ◽  
Tank Reactor ◽  
Shake Flasks ◽  
Liquid Mass Transfer ◽  
Ambient Gas

scholarly journals Circulating Jet for Enhancing the Mass Transfer in a Gas-liquid Stirred Tank Reactor

2021 ◽  
Author(s):  
Lei Wang ◽  
Xiao Xu ◽  
Hualin Wang ◽  
HongLai Liu ◽  
Qiang Yang
Keyword(s):  
Mass Transfer ◽  
Scale Up ◽  
Input Power ◽  
Stirred Tank ◽  
Exhaust Gas ◽  
Stirred Tank Reactor ◽  
Tank Reactor ◽  
Mass Transfer Efficiency ◽  
Low Mass ◽  
Liquid Mass Transfer

A gas-liquid stirred tank reactor (STR) has some problems, such as low mass transfer efficiency, high exhaust gas oxygen concentration, and low product conversion rate, due to limitations of stirring speed and input power. This article proposes a method to enhance the gas-liquid mass transfer in a STR using circulating jet internals. When a circulating jet is added, the average bubble size in the reactor is reduced to 1.26 mm, and the overall gas holdup is increased to 8.23%, which is an increase of 3.62 times of the original STR. The gas-liquid volumetric mass transfer coefficient is increased to 0.05556 s-1, which is 4.84 times of the original STR. The unit volume power is increased by only 1.4 times. These data provide references for the design and scale-up of new jet STRs.

The Production of Polyhydroxyalkanoates Using an Oscillatory Baffled Bioreactor

2009 ◽  
Vol 4 (5) ◽  
Author(s):  
Claudia N Troeger ◽  
Adam P Harvey
Keyword(s):  
Mass Transfer ◽  
Plug Flow ◽  
Stirred Tank ◽  
Agitation Rate ◽  
Additional Advantage ◽  
Stirred Tank Reactor ◽  
Capital Costs ◽  
Tank Reactor ◽  
Liquid Mass ◽  
Liquid Mass Transfer

Biopolymers are biodegradable polymers made from renewable resources. They can be made from plants or by microorganisms. Pseudomonas putida is one such microorganism. Under nutrient limitation and carbon excess it produces polyhydroxyalkanoates (PHA), which are linear polyesters, to store carbon as energy resource. PHAs are biodegradable and are used in the production of bioplastics.The production of biopolymers from microorganisms is currently not cost effective, because of expensive substrates, low yields and complex downstream processing. Therefore, intensification of PHA production to achieve higher yields in smaller, more efficient equipment at lower capital costs would be an advantage.Oscillatory baffled reactors (OBRs) are a form of plug flow reactor that can be used for long residence time reactions, as the plug flow is not dependent on achievement of a certain net flow velocity in the reactor, leading to much more compact designs. Fermentations, such as the production of PHA by P. putida, show characteristically long residence times. Therefore, they could be intensified in an OBR. Since one major advantage of the OBR is its highly uniform, controllable mixing, which can be used to enhance gas-to-liquid mass transfer, it should be suitable for biological reactions. Fed-batch or continuous fermentation is a second advantage, as it should minimize substrate and product inhibition. Lower, more uniform shear rates than in a stirred tank reactor would be an additional advantage for processing shear-sensitive cells. Previous research has shown that the OBR can provide higher biomass concentrations and greater gas-liquid mass transfer than conventional fermenters. In this work, the production of PHA by P. putida KT2442 was studied. The parameters investigated here were agitation rate and temperature. It was demonstrated that PHA can be produced in the OBR. The fermentation temperature and agitation rate resulting in the highest OD and PHA yield in this parameter space were determined as 30 ± 1°C and an oscillatory Reynolds number (Reo) of 300, respectively. It was also demonstrated that using an OBR can lead to higher OD (10.8 vs 7.26) and dry cell weight (3.75 g/L vs 2.4 g/L) than in a comparable conventional stirred tank reactor.

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