ORIGINAL RESEARCH: A dedicated vector for efficient library construction and high throughput screening in the hyphal fungus Chrysosporium lucknowense

2007 ◽  
Vol 3 (1) ◽  
pp. 48-57 ◽  
Author(s):  
Jan C. Verdoes ◽  
Peter J. Punt ◽  
Richard Burlingame ◽  
Jeffrey Bartels ◽  
Reijer van Dijk ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Original Research ◽  
Library Construction

scholarly journals Cas-CLIP: a method for customizing pooled CRISPR libraries

2018 ◽  
Author(s):  
Jiyeon Kweon ◽  
Da-eun Kim ◽  
An-Hee Jang ◽  
Yongsub Kim
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Target Genes ◽  
Biological Processes ◽  
Precise Method ◽  
Library Construction ◽  
Expensive Process ◽  
Screening Technology

ABSCTRACTAlthough pooled CRISPR libraries are widely used in high-throughput screening to study various biological processes, library construction for researcher’s own study is a time-consuming, labor-intensive, and expensive process. In this study, we develop a simple, scalable method, called Cas-CLIP, to customize conventional pooled CRISPR libraries using the CRISPR/Cas9 system. We show that conventional pooled CRISPR libraries can be modified by eliminating gRNAs that target positive genes, enabling the identification of unknown target genes in CRISPR screening. Cas-CLIP is a precise method for customizing conventional pooled CRISPR libraries and will broaden the scope of high-throughput screening technology.

scholarly journals Hi-TOM: a platform for high-throughput tracking of mutations induced by CRISPR/Cas systems

2017 ◽  
Author(s):  
Qing Liu ◽  
Chun Wang ◽  
Xiaozhen Jiao ◽  
Huawei Zhang ◽  
Lili Song ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Large Scale ◽  
High Reliability ◽  
Mutation Screening ◽  
Screening Strategy ◽  
Library Construction ◽  
Genetic Modifications ◽  
Parameter Configuration ◽  
Next Generation Sequencing Ngs

AbstractThe CRISPR/Cas system has been extensively applied to make precise genetic modifications in various organisms. Despite its importance and widespread use, large-scale mutation screening remains time-consuming, labour-intensive and costly. Here, we describe a cheap, practicable and high-throughput screening strategy that allows parallel screening of 96 × N (N denotes the number of targets) genome-modified sites. The strategy simplified and streamlined the process of next-generation sequencing (NGS) library construction by fixing the bridge sequences and barcoding primers. We also developed Hi-TOM (available at http://www.hi-tom.net/hi-tom/), an online tool to track the mutations with precise percentage. Analysis of the samples from rice, hexaploid wheat and human cells reveals that the Hi-TOM tool has high reliability and sensitivity in tracking various mutations, especially complex chimeric mutations that frequently induced by genome editing. Hi-TOM does not require specially design of barcode primers, cumbersome parameter configuration or additional data analysis. Thus, the streamlined NGS library construction and comprehensive result output make Hi-TOM particularly suitable for high-throughput identification of all types of mutations induced by CRISPR/Cas systems.

High-throughput screening program for botanical extracts

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
L Hingorani ◽  
NP Seeram ◽  
B Ebersole
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Screening Program ◽  
Botanical Extracts

High throughput screening of microbial biodiversity for the discovery of novel cosmeceutical agents

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
K Georgousaki ◽  
N DePedro ◽  
AM Chinchilla ◽  
N Aliagiannis ◽  
F Vicente ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Microbial Biodiversity

High throughput screening to investigate Brazilian Cerrado biome plant extract bank chemical diversity

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
LS Espindola ◽  
RG Dusi ◽  
KR Gustafson ◽  
J McMahon ◽  
JA Beutler
Keyword(s):  
High Throughput ◽  
Plant Extract ◽  
High Throughput Screening ◽  
Chemical Diversity ◽  
Brazilian Cerrado ◽  
Cerrado Biome

High-throughput screening revealed a clinically relevant drug to induce sperm motility

2014 ◽  
Author(s):  
Clair Cochrane ◽  
Halil Ruso ◽  
Anthony Hope ◽  
Rosemary G Clarke ◽  
Christopher Barratt ◽  
...  
Keyword(s):  
Sperm Motility ◽  
High Throughput ◽  
High Throughput Screening
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