Generation of Human Neutrophil Gelatinase-Associated Lipocalin Monoclonal Antibodies for Use in ARCHITECT® Assay

Hybridoma ◽  
2012 ◽  
Vol 31 (6) ◽  
pp. 436-442 ◽  
Author(s):  
David Hawksworth ◽  
Bailin Tu ◽  
Bryan Tieman ◽  
Susan Brophy ◽  
Joan Tyner ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Human Neutrophil ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Neutrophil Gelatinase

The solution structure and dynamics of human neutrophil gelatinase-associated lipocalin 1 1Edited by A. R. Fersht

1999 ◽  
Vol 289 (1) ◽  
pp. 139-157 ◽  
Author(s):  
Murray Coles ◽  
Tammo Diercks ◽  
Bernd Muehlenweg ◽  
Stefan Bartsch ◽  
Volker Zölzer ◽  
...  
Keyword(s):  
Human Neutrophil ◽  
Solution Structure ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Structure And Dynamics ◽  
Neutrophil Gelatinase

scholarly journals Human Neutrophil Gelatinase-Associated Lipocalin: A New Biomarker in Asthma-COPD Overlap Syndrome

2021 ◽  
Author(s):  
Serap Duru ◽  
FATMA UÇAR ◽  
Emine Bahar Kurt
Keyword(s):  
Human Neutrophil ◽  
Maximum Level ◽  
Overlap Syndrome ◽  
The Other ◽  
Ngal Level ◽  
Group Iii ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Group I ◽  
Severity Of The Disease ◽  
Neutrophil Gelatinase

Abstract AIM Human neutrophil gelatinase-associated lipocalin (NGAL) is a protein considered as a noninvasive prognostic biomarker in Asthma-COPD overlap syndrome (ACO). In this study we aimed to show the usability of NGAL as a biomarker in ACO characterized with airway inflammation. MATERIAL-METHOD: Three patient groups (Group I: COPD, n: 50, Group II: Asthma, n:50, Group III: ACO, n:50) and a healhty non smoker group (Group IV, n:50) are taken into study. The serum NGAL levels of groups are compared. In addition, in Group I,II,III the level of serum NGAL is compared with the age, duration of smoking (pocket/year), number of attack per year, respiratory function values, serum C reaktive protein (CRP, mg/dL), serum IgE (IU/mL), serum neutrophil (%) and serum lymphocyte (%), blood neutrophil-to-lymphocyte rate and atopy. FINDINGS: In patients having ACO when the maximum level of serum exist, it is observed that serum NGAL level is increased as the number of attacks is increased (p = 0.002). On the other hand in the patients having ACO there was also positive correlation between NLR and serum NGAL level (p < 0.05). There was no other relation found among the other variables. CONCULISION: In the chronic inflamatuar lung diseases the findings of increase in serum NGAL levels while the severity of the disease suggest us serum NGAL levels might be used as a biomarker for evaluating the inflammation.

scholarly journals Assessment of Renal function status in steady state Sickle Cell Anaemic children using urine human Neutrophil Gelatinase-associated Lipocalin and Albumin:Creatinine ratio.

2021 ◽  
Author(s):  
Olatubosun Oladipupo Olawale ◽  
Abiodun Folasade Adekanmbi ◽  
Ayobola Abimbola Sonuga ◽  
Oyebola Oluwagbemiga Sonuga ◽  
Samuel Olufemi Akodu ◽  
...  
Keyword(s):  
Renal Function ◽  
Steady State ◽  
Sickle Cell ◽  
Human Neutrophil ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Neutrophil Gelatinase

Expression, purification, crystallization and crystallographic characterization of dimeric and monomeric human neutrophil gelatinase associated lipocalin (NGAL)

1998 ◽  
Vol 54 (1) ◽  
pp. 93-95 ◽  
Author(s):  
R. K. Strong ◽  
T. Bratt ◽  
J. B. Cowland ◽  
N. Borregaard ◽  
F. C. Wiberg ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Human Neutrophil ◽  
Hanging Drop ◽  
Vapor Diffusion ◽  
Space Group ◽  
Lattice Constants ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Neutrophil Gelatinase

Crystals of the monomeric and dimeric forms of human neutrophil gelatinase associated lipocalin have been grown in hanging-drop vapor-diffusion trials using PEG as a precipitating agent with recombinant protein expressed in a baculovirus-based system. Crystals of monomeric NGAL belong to the cubic space group P432 with lattice constants a = b = c = 126.6 Å; crystals of dimeric NGAL belong to the tetragonal space group P41212 (or its enantiomorph P43212) with lattice constants a = b = 54.14 and c = 121.56 Å. Isomorphous crystals of the NGAL dimer can be grown in the presence of ligand: the tripeptide N-formyl-Met-Leu-Phe.

Development of monoclonal antibodies and simplified sandwich ELISA for canine neutrophil gelatinase-associated lipocalin A detection

2020 ◽  
Author(s):  
Jun Cao ◽  
Bo Tang ◽  
Xinyu Zhang ◽  
Xiaoli Xia ◽  
Huaichang Sun
Keyword(s):  
Monoclonal Antibodies ◽  
Sandwich Elisa ◽  
Kidney Injury ◽  
The Novel ◽  
E Coli ◽  
Neutrophil Gelatinase Associated Lipocalin ◽  
Hybridoma Cell Lines ◽  
Neutrophil Gelatinase ◽  
Canine Diseases ◽  
Good Agreement

Abstract Background Acute kidney injury (AKI) is one of the most common canine diseases with a high mortality rate. Neutrophil gelatinase-associated lipocalin (NGAL) is the novel biomarker for early diagnosis of renal injury. Only few sandwich ELISA kits are commercially available, all of which require the use of expensive enzyme-conjugated secondary or biotinylated antibody. The aims of this study were to develop high affinity monoclonal antibodies (mAbs) and simplified sandwich ELISA for canine NGAL detection. Results Recombinant canine NGAL was expressed in E. coli and purified to a high purity. Six hybridoma cell lines were generated by immunization of mice with the purified protein, all of which secreted high titers of specific mAbs. By screening 36 different antibody combinations, a pair of mAbs with high additivity and P/N ratio was selected as the capture and detection antibodies. By conjugation of the detection mAb with horse radish peroxidase, a simplified sandwich ELISA was developed with a correlation coefficient of 0.9939, detection limit of 8.28 ng/mL. The parallel test of 42 samples from healthy and AKI dogs showed the good agreement in NGAL concentrations detected by the simplified sandwich ELISA and commercial ELISA kit. Conclusions We developed canine NGAL-specific monoclonal antibodies and simplified sandwich ELISA. The simplified sandwich ELISA could replace the commercial ELISA kits for canine NGAL detection with the advantages of reduced cost and detection time.

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