SARS Coronavirus Nucleocapsid Protein Monoclonal Antibodies Developed Using a Prokaryotic Expressed Protein

Hybridoma ◽  
2011 ◽  
Vol 30 (5) ◽  
pp. 481-485 ◽  
Author(s):  
Juan Zhang ◽  
Ding Wang ◽  
Yue Li ◽  
Qing Zhao ◽  
Ailong Huang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Nucleocapsid Protein ◽  
Sars Coronavirus

scholarly journals Activation of AP-1 signal transduction pathway by SARS coronavirus nucleocapsid protein

2003 ◽  
Vol 311 (4) ◽  
pp. 870-876 ◽  
Author(s):  
Runtao He ◽  
Andrew Leeson ◽  
Anton Andonov ◽  
Yan Li ◽  
Nathalie Bastien ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Nucleocapsid Protein ◽  
Signal Transduction Pathway ◽  
Transduction Pathway ◽  
Sars Coronavirus

scholarly journals Nuclear/nucleolar localization properties of C-terminal nucleocapsid protein of SARS coronavirus

2005 ◽  
Vol 114 (1-2) ◽  
pp. 23-34 ◽  
Author(s):  
Khalid Amine Timani ◽  
Qingjiao Liao ◽  
Linbai Ye ◽  
Yingchun Zeng ◽  
Jing Liu ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Nucleolar Localization ◽  
Sars Coronavirus

scholarly journals Monoclonal Antibodies against Nucleocapsid Protein of SARS-CoV-2 Variants for Detection of COVID-19

2021 ◽  
Vol 22 (22) ◽  
pp. 12412
Author(s):  
Ruei-Min Lu ◽  
Shih-Han Ko ◽  
Wan-Yu Chen ◽  
Yu-Ling Chang ◽  
Hsiu-Ting Lin ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Nucleocapsid Protein ◽  
Limit Of Detection ◽  
Wide Spectrum ◽  
Mitigation Strategies ◽  
Respiratory Pathogens ◽  
Wild Type ◽  
Human Coronavirus ◽  
Spectrum Detection ◽  
Clinical Detection

Mitigation strategies of the coronavirus disease 2019 (COVID-19) pandemic have been greatly hindered by the continuous emergence of SARS-CoV-2 variants. New sensitive, rapid diagnostic tests for the wide-spectrum detection of viral variants are needed. We generated a panel of 41 monoclonal antibodies against the SARS-CoV-2 nucleocapsid protein (NP) by using mice hybridoma techniques. Of these mAbs, nine exhibited high binding activities and were applied in latex-based lateral flow immunoassays (LFIAs). The LFIAs utilizing NP-mAb-7 and -40 had the best sensitivity and lowest limit of detection: 8 pg for purified NP and 625 TCID50/mL for the authentic virus (hCoV-19/Taiwan/4/2020). The specificity tests showed that the NP-mAb-40/7 LFIA strips did not cross-react with five human coronavirus strains or 20 other common respiratory pathogens. Importantly, we found that 10 NP mutants, including alpha (B.1.1.7), beta (B.1.351), gamma (P.1), and delta (B.1.617.2) variants, could be detected by NP-mAb-40/7 LFIA strips. A clinical study (n = 60) of the NP-mAb-40/7 LFIA strips demonstrated a specificity of 100% and sensitivity of 90% in infected individuals with cycle threshold (Ct) values < 29.5. These anti-NP mAbs have strong potential for use in the clinical detection of SARS-CoV-2 infection, whether the virus is wild-type or a variant of concern.

scholarly journals The dimer interface of the SARS coronavirus nucleocapsid protein adapts a porcine respiratory and reproductive syndrome virus-like structure

FEBS Letters ◽  
2005 ◽  
Vol 579 (25) ◽  
pp. 5663-5668 ◽  
Author(s):  
Chung-ke Chang ◽  
Shih-Che Sue ◽  
Tsan-hung Yu ◽  
Chiu-Min Hsieh ◽  
Cheng-Kun Tsai ◽  
...  
Keyword(s):  
Nucleocapsid Protein ◽  
Sars Coronavirus ◽  
Dimer Interface ◽  
Porcine Respiratory

scholarly journals Recombinant Severe Acute Respiratory Syndrome (SARS) Coronavirus Nucleocapsid Protein Forms a Dimer through Its C-terminal Domain

2005 ◽  
Vol 280 (24) ◽  
pp. 23280-23286 ◽  
Author(s):  
I-Mei Yu ◽  
Christin L. T. Gustafson ◽  
Jianbo Diao ◽  
John W. Burgner ◽  
Zhihong Li ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Nucleocapsid Protein ◽  
Sars Coronavirus ◽  
Terminal Domain

scholarly journals The SARS coronavirus nucleocapsid protein – Forms and functions

2014 ◽  
Vol 103 ◽  
pp. 39-50 ◽  
Author(s):  
Chung-ke Chang ◽  
Ming-Hon Hou ◽  
Chi-Fon Chang ◽  
Chwan-Deng Hsiao ◽  
Tai-huang Huang
Keyword(s):  
Nucleocapsid Protein ◽  
Sars Coronavirus
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