Characterization and Application of Two Novel Monoclonal Antibodies Against Human CXCR4: Cell Proliferation and Migration Regulation for Glioma Cell Linein vitroby CXCR4/SDF-1α Signal

Hybridoma ◽  
2009 ◽  
Vol 28 (1) ◽  
pp. 33-41 ◽  
Author(s):  
Zhongqin Cheng ◽  
Shiyong Zhou ◽  
Xuefeng Wang ◽  
Fang Xie ◽  
Hongya Wu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Monoclonal Antibodies ◽  
Glioma Cell ◽  
Cell Proliferation And Migration ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals microRNA-451: A conditional switch controlling glioma cell proliferation and migration

Cell Cycle ◽  
2010 ◽  
Vol 9 (14) ◽  
pp. 2814-2820 ◽  
Author(s):  
Jakub Godlewski ◽  
Agnieszka Bronisz ◽  
Michal O. Nowicki ◽  
E. Antonio Chiocca ◽  
Sean Lawler
Keyword(s):  
Cell Proliferation ◽  
Glioma Cell ◽  
Cell Proliferation And Migration ◽  
Glioma Cell Proliferation ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals MiRNAs Mediate GDNF-Induced Proliferation and Migration of Glioma Cells

2017 ◽  
Vol 44 (5) ◽  
pp. 1923-1938 ◽  
Author(s):  
Bao-Le Zhang ◽  
Fu-Lu Dong ◽  
Ting-Wen Guo ◽  
Xiao-He Gu ◽  
Lin-Yan Huang ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Cell Adhesion ◽  
Glioma Cell ◽  
Glioma Cells ◽  
Cell Proliferation And Migration ◽  
Differentially Expressed Mirnas ◽  
Glioma Cell Proliferation ◽  
And Migration ◽  
Proliferation And Migration

Background/Aims: Glial cell line-derived neurotrophic factor (GDNF) is an important factor promoting invasive glioma growth. This study was performed to reveal a unique mechanism of glioma cell proliferation and migration. Methods: Human U251 glioma cells were used to screen the optimal GDNF concentration and treatment time to stimulate proliferation and migration. MicroRNA (MiRNA) expression profiles were detected by microarray and confirmed by real-time polymerase chain reaction (PCR). The target genes of differentially expressed miRNAs were predicted by miRWalk, and those targeted by multiple miRNAs were screened with Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. A regulatory miRNA network was constructed using ingenuity pathway analysis (IPA). Target gene expression of differentially expressed miRNAs was examined by real-time PCR or mRNA microarray. Results: The results show that 50 ng/mL GDNF for 24 h significantly promotes U251 glioma cell proliferation and migration (P < 0.05). Seven miRNAs (hsa-miR-194-5p, hsa-miR-152-3p, hsa-miR-205-5p, hsa-miR-629-5p, hsa-miR-3609, hsa-miR-183-5p, and hsa-miR-487b-3p) were significantly up-regulated after GDNF treatment (P < 0.05). These miRNAs are primarily involved in signal transduction, cell adhesion and cell cycle through mitogen-activated protein kinase (MAPK) signaling, focal adhesion and glioma signal pathways. Five of these miRNAs (hsa-miR-194-5p, hsa-miR-152-3p, hsa-miR-205-5p, hsa-miR-183-5p, and hsa-miR-487b-3p) co-regulate TP53 and Akt. mRNA expression levels of four genes co-targeted by two or more up-regulated miRNAs were significantly decreased after GDNF treatment (P < 0.05). Conclusion: GDNF treatment of U251 glioma cells significantly increased the expression of seven miRNAs involved in cell adhesion and the cell cycle.

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