Generation and Characterization of Monoclonal Antibodies to Human Keratinocyte Growth Factor Receptor

Hybridoma ◽  
2006 ◽  
Vol 25 (3) ◽  
pp. 115-124 ◽  
Author(s):  
Ping Wei ◽  
Jinghui Zhan ◽  
Shuying Liu ◽  
David Chang ◽  
Raj Haldankar ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Growth Factor ◽  
Keratinocyte Growth Factor ◽  
Growth Factor Receptor ◽  
Human Keratinocyte

scholarly journals Generation and Characterization of Rat Monoclonal Antibodies Against Epidermal Growth Factor Receptor

2015 ◽  
Vol 34 (6) ◽  
pp. 418-422 ◽  
Author(s):  
Tomohiro Osaki ◽  
Cai-Xia Wang ◽  
Taro Tachibana ◽  
Masayuki Azuma ◽  
Masaya Kitamura ◽  
...  
Keyword(s):  
Epidermal Growth Factor Receptor ◽  
Monoclonal Antibodies ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Growth Factor Receptor ◽  
Epidermal Growth

scholarly journals Bioactivity Determination of a Therapeutic Recombinant Human Keratinocyte Growth Factor by a Validated Cell-based Bioassay

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 699 ◽  
Author(s):  
Wenrong Yao ◽  
Ying Guo ◽  
Xi Qin ◽  
Lei Yu ◽  
Xinchang Shi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Lines ◽  
Hacat Cell ◽  
Keratinocyte Growth Factor ◽  
Growth Factor Receptor ◽  
Luciferase Reporter ◽  
Hematopoietic Stem ◽  
Human Keratinocyte ◽  
Serum Response ◽  
Hacat Cell Lines

The therapeutic recombinant human keratinocyte growth factor 1 (rhKGF-1) was approved by the FDA for oral mucositis resulting from hematopoietic stem cell transplantation for hematological malignancies in 2004. However, no recommended bioassay for rhKGF-1 bioactivity has been recorded in the U.S. Pharmacopoeia. In this study, we developed an rhKGF-1-dependent bioassay for determining rhKGF-1 bioactivity based on HEK293 and HaCat cell lines that stably expressed the luciferase reporter driven by the serum response element (SRE) and human fibroblast growth factor receptor (FGFR2) IIIb. A good responsiveness to rhKGF-1 and rhKGF-2 shared by target HEK293/HaCat cell lines was demonstrated. Our stringent validation was completely focused on specificity, linearity, accuracy, precision, and robustness according to the International Council for Harmonization (ICH) Q2 (R1) guidelines, AAPS/FDA Bioanalytical Workshop and the Chinese Pharmacopoeia. We confirmed the reliability of the method in determining rhKGF bioactivity. The validated method is highly timesaving, sensitive, and simple, and is especially valuable for providing information for quality control during the manufacture, research, and development of therapeutic rhKGF.

Expression, purification, and characterization of recombinant human keratinocyte growth factor-2 in Pichia pastoris

2007 ◽  
Vol 132 (1) ◽  
pp. 44-48 ◽  
Author(s):  
Yanchun Wang ◽  
Shengling Yuan ◽  
Peng Wang ◽  
Xiangxin Liu ◽  
Dewen Zhan ◽  
...  
Keyword(s):  
Growth Factor ◽  
Pichia Pastoris ◽  
Keratinocyte Growth Factor ◽  
Purification And Characterization ◽  
Human Keratinocyte

Construction and characterization of a high activity mutant of human keratinocyte growth factor-2

2009 ◽  
Vol 31 (6) ◽  
pp. 797-802 ◽  
Author(s):  
Jinfeng Wang ◽  
Xin Cai ◽  
Minji Zou ◽  
Tao Xu ◽  
Shen Liu ◽  
...  
Keyword(s):  
Growth Factor ◽  
High Activity ◽  
Keratinocyte Growth Factor ◽  
Human Keratinocyte

Generation and Characterization of a Panel of Monoclonal Antibodies Specific for Human Fibroblast Growth Factor Receptor 4 (FGFR4)

Hybridoma ◽  
2005 ◽  
Vol 24 (3) ◽  
pp. 152-159 ◽  
Author(s):  
Chaoyuan Chen ◽  
Sima Patel ◽  
Susanne Corisdeo ◽  
Xiangdong Liu ◽  
Holly Micolochick ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Human Fibroblast ◽  
Fibroblast Growth Factor Receptor ◽  
Growth Factor Receptor ◽  
Fibroblast Growth
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