Citrate-saline formulated mRNA delivery into the heart muscle with an electromechanical mapping and injection catheter does not lead to therapeutic effects in a porcine chronic myocardial ischemia model

2021 ◽  
Author(s):  
Niko Petteri Järveläinen ◽  
Paavo Juhani Halonen ◽  
Jussi Nurro ◽  
Olli-Pekka Hätinen ◽  
Henna Korpela ◽  
...  
Keyword(s):  
Myocardial Ischemia ◽  
Heart Muscle ◽  
Therapeutic Effects ◽  
Electromechanical Mapping ◽  
Chronic Myocardial Ischemia

scholarly journals Left Ventricular Electromechanical Mapping to Assess Efficacy of phVEGF 165 Gene Transfer for Therapeutic Angiogenesis in Chronic Myocardial Ischemia

Circulation ◽  
2000 ◽  
Vol 102 (9) ◽  
pp. 965-974 ◽  
Author(s):  
Peter R. Vale ◽  
Douglas W. Losordo ◽  
Charles E. Milliken ◽  
Michael Maysky ◽  
Darryl D. Esakof ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Myocardial Ischemia ◽  
Therapeutic Angiogenesis ◽  
Left Ventricular ◽  
Electromechanical Mapping ◽  
Chronic Myocardial Ischemia

scholarly journals Comparison of the Therapeutic Effects of Acupuncture at PC6 and ST36 for Chronic Myocardial Ischemia

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Li Shi ◽  
Jiliang Fang ◽  
Jiping Zhao ◽  
Guiyong Liu ◽  
Qing Zhao ◽  
...  
Keyword(s):  
Myocardial Ischemia ◽  
Troponin T ◽  
Left Ventricular ◽  
Left Ventricular Ejection ◽  
Therapeutic Effects ◽  
Ventricular Ejection Fraction ◽  
Systolic Volume ◽  
End Diastolic Volume ◽  
End Systolic Volume ◽  
Chronic Myocardial Ischemia

We aimed to compare the differences of the effects on chronic myocardial ischemia (MI) of acupuncture at PC6 and ST36. The chronic MI model of minipigs was created by implanting an Ameroid constrictor on the left anterior descending coronary artery (LAD) and then two weeks’ acupuncture was stimulated at PC6 or ST36, respectively. The results showed that both acupoints’ stimulation decreased the serous cardiac troponin T (cTnT) and ischemia modified albumin (IMA) significantly and improved the ischemic ECG changes. The amplitude of pathological Q wave in the PC6 group decreased more significantly than that of the ST36 group. The cardiovascular magnetic resonance imaging (cMRI) results showed that the decreased left ventricular ejection fraction (LVEF) was not improved obviously in both groups. The left ventricular end-diastolic volume (LVEDV) and left ventricular end-systolic volume (LVESV) enlarged progressively even after acupuncture. The left ventricular wall mass (LVWM) in the ST36 group increased more obviously than that of the PC6 group, which paralleled the decreasing angiotensin II (Ang II) concentration in the plasma. These results suggested that acupuncture at PC6 or ST36 was effective for protecting the myocardium from chronic ischemic injury, and the effect of PC6 seemed to be better.

Combined VEGF and FGF-2 therapy promotes dose-dependently angiogenesis and function in chronic myocardial ischemia

2004 ◽  
Vol 52 (S 1) ◽  
Author(s):  
C Heilmann ◽  
S Tascher ◽  
L Bredow ◽  
T Renner ◽  
H G�bel ◽  
...  
Keyword(s):  
Myocardial Ischemia ◽  
And Function ◽  
Chronic Myocardial Ischemia

Abstract 19885: Impact of Cardiovascular Risk Factors on rAAV.Thymosin ß4 Mediated Vascular Regeneration in Large Animals

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Rabea Hinkel ◽  
Andrea Howe ◽  
Sarah Straub ◽  
Wira Husada ◽  
Seugmin Lee ◽  
...  
Keyword(s):  
Risk Factors ◽  
Cardiovascular Risk ◽  
Myocardial Ischemia ◽  
Cardiovascular Risk Factors ◽  
Myocardial Function ◽  
Regional Myocardial Function ◽  
Transgenic Pigs ◽  
Circumflex Artery ◽  
Vascular Regeneration ◽  
Chronic Myocardial Ischemia

Diabetes and hypercholesterolemia are two of the major risk factors for developing cardiovascular disease, especially in combination with chronic myocardial ischemia it represents one of the most common causes of disability or death. New pro-angiogenic factors like Thymosin ß4 (Tß4), a small 4.9 kDa peptide influences cell motility and migration might be suitable for inducing therapeutic neovascularization in chronic myocardial ischemia a cardiovascular risk factors. Methods: Chronic ischemia was induced via reduction stent graft in the circumflex artery, leading to a total occlusion on day 28 (d28). Beside wildtyp animals (wt) ± high fat diet (hypercholesteric (hyp), choselsterol 78±1 wt vs 90±2 hyp), d iabetic transgenic pigs were used (increased blood glucose 305 ±12 mg/dL) . Regional application of rAAV Tß4 (5x10E12 viral particles, d28) was performed in wt, hyp and diabetic pigs respectively. Global myocardial function was obtained at day 28 and 56. Regional myocardial function and post mortem angiography were examined on day 56. Histological analysis of capillary density (capillaries/field (C/F)) was performed in the ischemic tissue. Results: rAAV.Tß4 transduction significantly enhanced capillaries (278±6 vs. 148±6 c/hpf) and collaterals (9±1 vs. 3±1) compared to control wt animals. Furthermore, global (EF: 47±4 vs. 29±3 %); and regional myocardial function (SES at 150 b/min: 73±5 vs. 10±6 % of non-ischemic area) were increased after Tß4 overexpression. In the models of cardiovascular risk factors and chronic myocardial ischemia increased neovascularization and improved myocardial function could be achieved after rAAV Tß4 application, albeit at a lower level (capillaries: diabetic: 190±4 (Tß4) vs. 120±5 (control) c/hpf hyp: 188±6 (Tß4) vs 130±12 (control) c/hpf; collaterals: diabetic: 4±1 (Tß4) vs. 2±1 (control) hyp: 6±1 (Tß4) vs 3±1 (control); EF: diabetic: 32±2 % (Tß4) vs. 27±1 % (control) hyp: 42±4 % (Tß4) vs 28±3 % (control)). Conclusion: Long term Thymosin ß4 expression induced neovascularization and improved myocardial perfusion and function even in pigs with additional risk factors. Therefore, rAAV.Tß4 appears suitable for treatment of ischemic cardiomyopathy associated with these cardiovascular risk factors.

Clinical Gene and Stem Cell Therapy in Patients with Acute and Chronic Myocardial Ischemia

2014 ◽  
pp. 143-167
Author(s):  
Jens Kastrup ◽  
Annette Ekblond ◽  
Mandana Haack-Sørensen ◽  
Anders B. Mathiasen ◽  
Abbas A. Qayyum
Keyword(s):  
Stem Cell ◽  
Myocardial Ischemia ◽  
Cell Therapy ◽  
Stem Cell Therapy ◽  
Chronic Myocardial Ischemia
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