scholarly journals Development of Anti-Human Mesothelin-Targeted Chimeric Antigen Receptor Messenger RNA–Transfected Peripheral Blood Lymphocytes for Ovarian Cancer Therapy

2018 ◽  
Vol 29 (5) ◽  
pp. 614-625 ◽  
Author(s):  
Chien-Fu Hung ◽  
Xuequn Xu ◽  
Linhong Li ◽  
Ying Ma ◽  
Qiu Jin ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Therapy ◽  
Chimeric Antigen Receptor ◽  
Peripheral Blood ◽  
Messenger Rna ◽  
Antigen Receptor ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes

A Preliminary Study on the Functional Analysis of Peripheral Blood Lymphocytes from Ovarian Cancer Patients Developing Hama after Immunoscintigraphy

1994 ◽  
Vol 9 (2) ◽  
pp. 115-120 ◽  
Author(s):  
B. Donnerstag ◽  
R.P. Baum ◽  
J.B. Oltrogge ◽  
A. Hertel ◽  
G. Hör
Keyword(s):  
Ovarian Cancer ◽  
Functional Analysis ◽  
Cytotoxic Activity ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Ca 125 ◽  
Blood Lymphocytes ◽  
Tumor Associated Antigen

In the follow-up of ovarian cancer patients, rising levels of the tumor-associated antigen CA 125 are an indication for immunoscintigraphy. Human anti-mouse antibodies (HAMA) are frequently found after immunoscintigraphy with murine MAb directed against CA 125. Since we observed that patients developing high HAMA-levels in serum remained free of tumor or had stable disease, we examined the cytotoxic activity of peripheral blood lymphocytes (PBL) by a fluorescence-based assay. Our results demonstrate that PBLs of patients with high anti-idiotypic antibodies show an increased cytotoxic activity (by a factor of 4) compared to those of patients with low HAMA levels. The clinical course of the patients after the first injection of murine monoclonal antibody was observed over a period of 1 to 3 years. Improvement or deterioration of patients ‘ clinical condition corresponded with the results obtained by functional analysis. Further investigations concerning the course of cytotoxic activity in HAMA-positive patients will have to clarify HAMA's role in the immune response.

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