scholarly journals Manufacturing and Characterization of a Recombinant Adeno-Associated Virus Type 8 Reference Standard Material

2014 ◽  
Vol 25 (11) ◽  
pp. 977-987 ◽  
Author(s):  
Eduard Ayuso ◽  
Véronique Blouin ◽  
Martin Lock ◽  
Susan McGorray ◽  
Xavier Leon ◽  
...  
Keyword(s):  
Virus Type ◽  
Reference Standard ◽  
Adeno Associated Virus ◽  
Standard Material

scholarly journals Characterization of a Recombinant Adeno-Associated Virus Type 2 Reference Standard Material

2010 ◽  
Vol 21 (10) ◽  
pp. 1273-1285 ◽  
Author(s):  
Martin Lock ◽  
Susan McGorray ◽  
Alberto Auricchio ◽  
Eduard Ayuso ◽  
E. Jeffrey Beecham ◽  
...  
Keyword(s):  
Virus Type ◽  
Reference Standard ◽  
Adeno Associated Virus ◽  
Standard Material

Stability of the adeno-associated virus 8 reference standard material

Gene Therapy ◽  
2019 ◽  
Vol 26 (5) ◽  
pp. 211-215 ◽  
Author(s):  
Magalie Penaud-Budloo ◽  
Frédéric Broucque ◽  
Katell Harrouet ◽  
Mohammed Bouzelha ◽  
Sylvie Saleun ◽  
...  
Keyword(s):  
Reference Standard ◽  
Adeno Associated Virus ◽  
Standard Material

Establishment and characterization of KB cell lines latently infected with adeno-associated virus type 1

Virology ◽  
1977 ◽  
Vol 82 (1) ◽  
pp. 84-92 ◽  
Author(s):  
Hiroshi Handa ◽  
Kazuko Shiroki ◽  
Hiroto Shimojo
Keyword(s):  
Cell Lines ◽  
Virus Type ◽  
Adeno Associated Virus ◽  
Latently Infected

Cloning and Characterization of Adeno-Associated Virus Type 5

1999 ◽  
Vol 73 (2) ◽  
pp. 1309-1319 ◽  
Author(s):  
John A. Chiorini ◽  
Frank Kim ◽  
Linda Yang ◽  
Robert M. Kotin
Keyword(s):  
Virus Type ◽  
The Other ◽  
Adeno Associated Virus ◽  
Variable Regions ◽  
Reading Frame ◽  
Rep Proteins ◽  
Dna Binding Site ◽  
Particle Stability ◽  
Serological Data

ABSTRACT Adeno-associated virus type 5 (AAV5) is distinct from other dependovirus serotypes based on DNA hybridization and serological data. To better understand the biology of AAV5, we have cloned and sequenced its genome and generated recombinant AAV5 particles. The single-stranded DNA genome is similar in length and genetic organization to that of AAV2. The rep gene of AAV5 is 67% homologous to AAV2, with the majority of the changes occurring in the carboxyl and amino termini. This homology is much less than that observed with other reported AAV serotypes. The inverted terminal repeats (ITRs) are also unique compared to those of the other AAV serotypes. While the characteristic AAV hairpin structure and the Rep DNA binding site are retained, the consensus terminal resolution site is absent. These differences in the Rep proteins and the ITRs result in a lack of cross-complementation between AAV2 and AAV5 as measured by the production of recombinant AAV particles. Alignment of the cap open reading frame with that of the other AAV serotypes identifies both conserved and variable regions which could affect tissue tropism and particle stability. Comparison of transduction efficiencies in a variety of cells lines and a lack of inhibition by soluble heparin indicate that AAV5 may utilize a distinct mechanism of uptake compared to AAV2.

scholarly journals Characterization of the Transcription Profile of Adeno-Associated Virus Type 5 Reveals a Number of Unique Features Compared to Previously Characterized Adeno-Associated Viruses

2002 ◽  
Vol 76 (24) ◽  
pp. 12435-12447 ◽  
Author(s):  
Jianming Qiu ◽  
Ramnath Nayak ◽  
Gregory E. Tullis ◽  
David J. Pintel
Keyword(s):  
Virus Type ◽  
Transcription Initiation ◽  
Infectious Clone ◽  
Initiation Site ◽  
Inverted Terminal Repeat ◽  
Transcription Profile ◽  
Adeno Associated Virus ◽  
Rep Protein ◽  
A Site

ABSTRACT We report the initial characterization of adeno-associated virus type 5 (AAV5) RNAs generated following viral infection and the construction of a replicating infectious clone of AAV5. While the basic transcription profile of AAV5 was similar to that of AAV2, there were also significant differences. Mapping of the AAV5 transcripts demonstrated an efficient transcription initiation site within the AAV5 inverted terminal repeat (ITR), and mapping of the AAV5 intron revealed that it is considerably smaller than that of AAV2. Furthermore, in contrast to the case for AAV2, neither the Rep protein nor additional adenovirus gene products were required to achieve efficient promoter activity and pre-mRNA splicing following transfection of an AAV5 rep/cap plasmid clone lacking the ITRs into 293 cells. Perhaps most surprisingly, RNAs generated from both the AAV5 P7 and P19 promoters were efficiently polyadenylated at a site lying within the intronic region in the center of the genome. Because P7- and P19-generated transcripts are polyadenylated at this site and not spliced, Rep78 and Rep52 were the only Rep proteins detected during AAV5 infection.

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