Enhanced Therapeutic Effects Conferred by an Experimental DNA Vaccine Targeting Human Papillomavirus-Induced Tumors

2013 ◽  
Vol 24 (10) ◽  
pp. 861-870 ◽  
Author(s):  
Mariana O. Diniz ◽  
Francisco A.M.O. Cariri ◽  
Luana R.M.M. Aps ◽  
Luís C.S. Ferreira
Keyword(s):  
Human Papillomavirus ◽  
Dna Vaccine ◽  
Therapeutic Effects ◽  
Induced Tumors

scholarly journals Enhanced Prophylactic and Therapeutic Effects of Polylysine-Modified Ara h 2 DNA Vaccine in a Mouse Model of Peanut Allergy

2016 ◽  
Vol 171 (3-4) ◽  
pp. 241-250 ◽  
Author(s):  
Zhenlai Zhu ◽  
Jinlei Yu ◽  
Yuan Niu ◽  
Shuhong Sun ◽  
Yufeng Liu ◽  
...  
Keyword(s):  
Mouse Model ◽  
Dna Vaccine ◽  
Peanut Allergy ◽  
Therapeutic Effects ◽  
Ara H 2

Development of a novel viral DNA vaccine against human papillomavirus: AcHERV-HP16L1

Vaccine ◽  
2010 ◽  
Vol 28 (6) ◽  
pp. 1613-1619 ◽  
Author(s):  
Hee-Jung Lee ◽  
Nuri Park ◽  
Hee-Jeong Cho ◽  
Jong Kwang Yoon ◽  
Nguyen Dinh Van ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Dna Vaccine ◽  
Viral Dna

scholarly journals Immune Responses and Therapeutic Antitumor Effects of an Experimental DNA Vaccine Encoding Human Papillomavirus Type 16 Oncoproteins Genetically Fused to Herpesvirus Glycoprotein D

2010 ◽  
Vol 17 (10) ◽  
pp. 1576-1583 ◽  
Author(s):  
Mariana O. Diniz ◽  
Marcio O. Lasaro ◽  
Hildegund C. Ertl ◽  
Luís C. S. Ferreira
Keyword(s):  
Human Papillomavirus ◽  
T Cell ◽  
Immune Responses ◽  
Dna Vaccine ◽  
T Cell Responses ◽  
Glycoprotein D ◽  
Antitumor Effects ◽  
Hpv 16 ◽  
Human Papillomavirus Type 16 ◽  
Cell Responses

ABSTRACT Recombinant adenovirus or DNA vaccines encoding herpes simplex virus type 1 (HSV-1) glycoprotein D (gD) genetically fused to human papillomavirus type 16 (HPV-16) oncoproteins (E5, E6, and E7) induce antigen-specific CD8+ T-cell responses and confer preventive resistance to transplantable murine tumor cells (TC-1 cells). In the present report, we characterized some previously uncovered aspects concerning the induction of CD8+ T-cell responses and the therapeutic anticancer effects achieved in C57BL/6 mice immunized with pgD-E7E6E5 previously challenged with TC-1 cells. Concerning the characterization of the immune responses elicited in mice vaccinated with pgD-E7E6E5, we determined the effect of the CD4+ T-cell requirement, longevity, and dose-dependent activation on the E7-specific CD8+ T-cell responses. In addition, we determined the priming/boosting properties of pgD-E7E6E5 when used in combination with a recombinant serotype 68 adenovirus (AdC68) vector encoding the same chimeric antigen. Mice challenged with TC-1 cells and then immunized with three doses of pgD-E7E6E5 elicited CD8+ T-cell responses, measured by intracellular gamma interferon (IFN-γ) and CD107a accumulation, to the three HPV-16 oncoproteins and displayed in vivo antigen-specific cytolytic activity, as demonstrated with carboxyfluorescein diacetate succinimidyl ester (CFSE)-labeled target cells pulsed with oligopeptides corresponding to the H-2Db -restricted immunodominant epitopes of the E7, E6, or E5 oncoprotein. Up to 70% of the mice challenged with 5 × 105 TC-1 cells and immunized with pgD-E7E6E5 controlled tumor development even after 3 days of tumor cell challenge. In addition, coadministration of pgD-E7E6E5 with DNA vectors encoding pGM-CSF or interleukin-12 (IL-12) enhanced the therapeutic antitumor effects for all mice challenged with TC-1 cells. In conclusion, the present results expand our previous knowledge on the immune modulation properties of the pgD-E7E6E5 vector and demonstrate, for the first time, the strong antitumor effects of the DNA vaccine, raising promising perspectives regarding the development of immunotherapeutic reagents for the control of HPV-16-associated tumors.

A DNA vaccine based on a shuffled E7 oncogene of the human papillomavirus type 16 (HPV 16) induces E7-specific cytotoxic T cells but lacks transforming activity

Vaccine ◽  
2001 ◽  
Vol 19 (30) ◽  
pp. 4276-4286 ◽  
Author(s):  
Wolfram Osen ◽  
Tanja Peiler ◽  
Peter Öhlschläger ◽  
Sandra Caldeira ◽  
Stefan Faath ◽  
...  
Keyword(s):  
T Cells ◽  
Human Papillomavirus ◽  
Dna Vaccine ◽  
Cytotoxic T Cells ◽  
Hpv 16 ◽  
Human Papillomavirus Type 16 ◽  
Transforming Activity ◽  
E7 Oncogene

Chemo-immunotherapy using saffron and its ingredients followed by E7-NT (gp96) DNA vaccine generates different anti-tumor effects against tumors expressing the E7 protein of human papillomavirus

2014 ◽  
Vol 160 (2) ◽  
pp. 499-508 ◽  
Author(s):  
Afshin Khavari ◽  
Azam Bolhassani ◽  
Fatemeh Alizadeh ◽  
S. Zahra Bathaie ◽  
Prabha Balaram ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Dna Vaccine

Protection against human papillomavirus type 16-induced tumors in mice using non-genetically modified lactic acid bacteria displaying E7 antigen at its surface

2012 ◽  
Vol 97 (3) ◽  
pp. 1231-1239 ◽  
Author(s):  
Pedro Ribelles ◽  
Bouasria Benbouziane ◽  
Philippe Langella ◽  
Juan E. Suárez ◽  
Luis G. Bermúdez-Humarán ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Human Papillomavirus ◽  
Lactic Acid Bacteria ◽  
Genetically Modified ◽  
Human Papillomavirus Type 16 ◽  
Induced Tumors ◽  
E7 Antigen
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