scholarly journals Transgene Expression up to 7 Years in Nonhuman Primates Following Hepatic Transduction with Helper-Dependent Adenoviral Vectors

2013 ◽  
Vol 24 (8) ◽  
pp. 761-765 ◽  
Author(s):  
Nicola Brunetti-Pierri ◽  
Thomas Ng ◽  
David Iannitti ◽  
William Cioffi ◽  
Gary Stapleton ◽  
...  
Keyword(s):  
Transgene Expression ◽  
Nonhuman Primates ◽  
Adenoviral Vectors

scholarly journals Five Years of Successful Inducible Transgene Expression Following Locoregional Adeno-Associated Virus Delivery in Nonhuman Primates with No Detectable Immunity

2019 ◽  
Vol 30 (7) ◽  
pp. 802-813 ◽  
Author(s):  
Mickaël Guilbaud ◽  
Marie Devaux ◽  
Celia Couzinié ◽  
Johanne Le Duff ◽  
Alice Toromanoff ◽  
...  
Keyword(s):  
Transgene Expression ◽  
Nonhuman Primates ◽  
Adeno Associated Virus ◽  
Virus Delivery

scholarly journals Herpes Simplex Virus Type 1 Thymidine Kinase Sequence Fused to the lacZ Gene Increases Levels of β-Galactosidase Activity per Genome of High-Capacity but Not First-Generation Adenoviral Vectors In Vitro and In Vivo

2008 ◽  
Vol 83 (4) ◽  
pp. 2004-2010 ◽  
Author(s):  
M. Puntel ◽  
R. J. Barrett ◽  
S. Mondkar ◽  
V. Saxena ◽  
K. M. Kroeger ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Transgene Expression ◽  
First Generation ◽  
High Capacity ◽  
Adenoviral Vectors ◽  
Simplex Virus

ABSTRACT Increased transgene expression per vector genome is an important goal in the optimization of viral vectors for gene therapy. Herein we demonstrate that herpes simplex virus type 1 (HSV1) thymidine kinase (TK) gene sequences (1,131 bp) fused to the 3′ end of lacZ increase transgene expression from high-capacity adenoviral vectors (HCAd), but not from first-generation (Ad) vectors. The woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), in contrast, increased transgene expression levels from Ad but not HCAd vectors. The differential activity of the HSV1 TK gene and WPRE sequences was detected both in vitro and in vivo and suggests potentially different mechanisms of action or the interaction of these elements with vector genomic sequences.

A small chimeric promoter for high prostate-specific transgene expression from adenoviral vectors

The Prostate ◽  
2007 ◽  
Vol 67 (8) ◽  
pp. 829-839 ◽  
Author(s):  
Robert Kraaij ◽  
Laura van der Weel ◽  
Corrina M.A. de Ridder ◽  
Hetty A.G.M. van der Korput ◽  
Joke L.M. Zweistra ◽  
...  
Keyword(s):  
Transgene Expression ◽  
Adenoviral Vectors ◽  
Chimeric Promoter

scholarly journals Reduced Inflammatory Reactions to the Inoculation of Helper-Dependent Adenoviral Vectors in Traumatically Injured Rat Brain

2002 ◽  
Vol 22 (8) ◽  
pp. 959-970 ◽  
Author(s):  
Linglong Zou ◽  
Patricia Yotnda ◽  
Tiejun Zhao ◽  
Xiaoqing Yuan ◽  
Yan Long ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
Rat Brain ◽  
Neutralizing Antibodies ◽  
Transgene Expression ◽  
First Generation ◽  
High Efficiency ◽  
Adenoviral Vectors ◽  
Tissue Loss ◽  
Inflammatory Reactions

Traumatic brain injury (TBI) causes delayed neuronal deficits that in principle could be prevented by timely intervention with therapeutic genes. However, appropriate vectors for gene transfer to the brain with TBI remain to be developed. First-generation adenoviruses (fgAd) are usually associated with inflammatory and toxic effects when inoculated into brains, despite their high efficiency of gene transfer to these tissues. In this study the authors attempted to determine whether a less immunogenic gene-transfer protocol can be established in the traumatically injured rat brain using helper-dependent adenoviruses (hdAd), a novel adenoviral construct with full deletion of viral coding sequences. Their results show that transgene expression from intrahippocampally inoculated hdAd is maintained for at least 2 months after TBI, in contrast to the much shorter duration of fgAd-mediated gene expression. There was only minimal secretion of proinflammatory IL-1β and TNF-α after inoculation of hdAd. Furthermore, the hdAd-mediated gene expression was associated with less microglial proliferation, astrocytic activation, and macrophage infiltration than observed in fgAd-inoculated brains. There was no additional tissue loss after hdAd inoculation compared with PBS injection. Although both anti-adenoviral and neutralizing antibodies were found in serum after brain inoculation of hdAd, they did not appear to affect transgene expression. The results suggest that hdAd are less immunogenic vectors than conventional adenoviral vectors, and offer improved vehicles for long-term therapeutic transgene transfer to traumatically injured brains.

scholarly journals A novel system for mitigation of ectopic transgene expression induced by adenoviral vectors

Gene Therapy ◽  
2001 ◽  
Vol 8 (16) ◽  
pp. 1271-1275 ◽  
Author(s):  
PN Reynolds ◽  
MD Holmes ◽  
Y Adachi ◽  
L Kaliberova ◽  
DT Curiel
Keyword(s):  
Transgene Expression ◽  
Adenoviral Vectors
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