HIV-1-Derived Lentiviral Vectors Directly Activate Plasmacytoid Dendritic Cells, Which in Turn Induce the Maturation of Myeloid Dendritic Cells

2011 ◽  
Vol 22 (2) ◽  
pp. 177-188 ◽  
Author(s):  
Maura Rossetti ◽  
Silvia Gregori ◽  
Ehud Hauben ◽  
Brian D. Brown ◽  
Lucia Sergi Sergi ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Lentiviral Vectors ◽  
Plasmacytoid Dendritic Cells ◽  
Myeloid Dendritic Cells ◽  
Hiv 1

scholarly journals Human Immunodeficiency Virus Type 1 Activates Plasmacytoid Dendritic Cells and Concomitantly Induces the Bystander Maturation of Myeloid Dendritic Cells

2004 ◽  
Vol 78 (10) ◽  
pp. 5223-5232 ◽  
Author(s):  
Jean-François Fonteneau ◽  
Marie Larsson ◽  
Anne-Sophie Beignon ◽  
Kelli McKenna ◽  
Ida Dasilva ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Dendritic Cells ◽  
T Cell ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Plasmacytoid Dendritic Cells ◽  
Myeloid Dendritic Cells ◽  
Immunodeficiency Virus ◽  
Hiv 1

ABSTRACT In this study, we analyzed the phenotypic and physiological consequences of the interaction of plasmacytoid dendritic cells (pDCs) with human immunodeficiency virus type 1 (HIV-1). pDCs are one cellular target of HIV-1 and respond to the virus by producing alpha/beta interferon (IFN-α/β) and chemokines. The outcome of this interaction, notably on the function of bystander myeloid DC (CD11c+ DCs), remains unclear. We therefore evaluated the effects of HIV-1 exposure on these two DC subsets under various conditions. Blood-purified pDCs and CD11c+ DCs were exposed in vitro to HIV-1, after which maturation markers, cytokine production, migratory capacity, and CD4 T-cell stimulatory capacity were analyzed. pDCs exposed to different strains of infectious or even chemically inactivated, nonreplicating HIV-1 strongly upregulated the expression of maturation markers, such as CD83 and functional CCR7, analogous to exposure to R-848, a synthetic agonist of toll-like receptor-7 and -8. In addition, HIV-1-activated pDCs produced cytokines (IFN-α and tumor necrosis factor alpha), migrated in response to CCL19 and, in coculture, matured CD11c+ DCs, which are not directly activated by HIV. pDCs also acquired the ability to stimulate naïve CD4+ T cells, albeit less efficiently than CD11c+ DCs. This HIV-1-induced maturation of both DC subsets may explain their disappearance from the blood of patients with high viral loads and may have important consequences on HIV-1 cellular transmission and HIV-1-specific T-cell responses.

scholarly journals Human Plasmacytoid Dendritic Cells Efficiently Capture HIV-1 Envelope Glycoproteins via CD4 for Antigen Presentation

2013 ◽  
Vol 191 (1) ◽  
pp. 60-69 ◽  
Author(s):  
Kerrie J. Sandgren ◽  
Anna Smed-Sörensen ◽  
Mattias N. Forsell ◽  
Martina Soldemo ◽  
William C. Adams ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Antigen Presentation ◽  
Plasmacytoid Dendritic Cells ◽  
Envelope Glycoproteins ◽  
Hiv 1

scholarly journals Activation of Plasmacytoid Dendritic Cells with TLR9 Agonists Initiates Invariant NKT Cell-Mediated Cross-Talk with Myeloid Dendritic Cells

2006 ◽  
Vol 177 (2) ◽  
pp. 1028-1039 ◽  
Author(s):  
Carlos J. Montoya ◽  
Hyun-Bae Jie ◽  
Lena Al-Harthi ◽  
Candice Mulder ◽  
Pablo J. Patiño ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Cross Talk ◽  
Plasmacytoid Dendritic Cells ◽  
Myeloid Dendritic Cells ◽  
Nkt Cell ◽  
Invariant Nkt Cell

scholarly journals Quercetin Administration Suppresses the Cytokine Storm in Myeloid and Plasmacytoid Dendritic Cells

2021 ◽  
Vol 22 (15) ◽  
pp. 8349
Author(s):  
Giulio Verna ◽  
Marina Liso ◽  
Elisabetta Cavalcanti ◽  
Giusy Bianco ◽  
Veronica Di Sarno ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Intestinal Barrier ◽  
Plasmacytoid Dendritic Cells ◽  
Myeloid Dendritic Cells ◽  
Soluble Factors ◽  
Secreted Factors ◽  
Mucosal Tissues ◽  
Basolateral Side ◽  
Bowel Diseases ◽  
Inflammatory Bowel

Dendritic cells (DCs) can be divided by lineage into myeloid dendritic cells (mDCs) and plasmacytoid dendritic cells (pDCs). They both are present in mucosal tissues and regulate the immune response by secreting chemokines and cytokines. Inflammatory bowel diseases (IBDs) are characterized by a leaky intestinal barrier and the consequent translocation of bacterial lipopolysaccharide (LPS) to the basolateral side. This results in DCs activation, but the response of pDCs is still poorly characterized. In the present study, we compared mDCs and pDCs responses to LPS administration. We present a broad panel of DCs secreted factors, including cytokines, chemokines, and growth factors. Our recent studies demonstrated the anti-inflammatory effects of quercetin administration, but to date, there is no evidence about quercetin’s effects on pDCs. The results of the present study demonstrate that pDCs can respond to LPS and that quercetin exposure modulates soluble factors release through the same molecular pathway used by mDCs (Slpi, Hmox1, and AP-1).

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