A Lentiviral Strategy for Highly Efficient Retrograde Gene Transfer by Pseudotyping with Fusion Envelope Glycoprotein

2011 ◽  
Vol 22 (2) ◽  
pp. 197-206 ◽  
Author(s):  
Shigeki Kato ◽  
Kenta Kobayashi ◽  
Ken-ichi Inoue ◽  
Masahito Kuramochi ◽  
Tomoaki Okada ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Envelope Glycoprotein ◽  
Highly Efficient ◽  
Retrograde Gene Transfer

scholarly journals Retrograde Transgene Expression via Neuron-Specific Lentiviral Vector Depends on Both Species and Input Projections

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1387
Author(s):  
Yukiko Otsuka ◽  
Hitomi Tsuge ◽  
Shiori Uezono ◽  
Soshi Tanabe ◽  
Maki Fujiwara ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Immune Responses ◽  
Envelope Glycoprotein ◽  
Transgene Expression ◽  
Histological Analysis ◽  
Lentiviral Vector ◽  
Cortical Input ◽  
Input System ◽  
Vesicular Stomatitis ◽  
Retrograde Gene Transfer

For achieving retrograde gene transfer, we have so far developed two types of lentiviral vectors pseudotyped with fusion envelope glycoprotein, termed HiRet vector and NeuRet vector, consisting of distinct combinations of rabies virus and vesicular stomatitis virus glycoproteins. In the present study, we compared the patterns of retrograde transgene expression for the HiRet vs. NeuRet vectors by testing the cortical input system. These vectors were injected into the motor cortex in rats, marmosets, and macaques, and the distributions of retrograde labels were investigated in the cortex and thalamus. Our histological analysis revealed that the NeuRet vector generally exhibits a higher efficiency of retrograde gene transfer than the HiRet vector, though its capacity of retrograde transgene expression in the macaque brain is unexpectedly low, especially in terms of the intracortical connections, as compared to the rat and marmoset brains. It was also demonstrated that the NeuRet but not the HiRet vector displays sufficiently high neuron specificity and causes no marked inflammatory/immune responses at the vector injection sites in the primate (marmoset and macaque) brains. The present results indicate that the retrograde transgene efficiency of the NeuRet vector varies depending not only on the species but also on the input projections.

scholarly journals Highly Efficient Retrograde Gene Transfer into Motor Neurons by a Lentiviral Vector Pseudotyped with Fusion Glycoprotein

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e75896 ◽  
Author(s):  
Miyabi Hirano ◽  
Shigeki Kato ◽  
Kenta Kobayashi ◽  
Tomoaki Okada ◽  
Hiroyuki Yaginuma ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Motor Neurons ◽  
Lentiviral Vector ◽  
Highly Efficient ◽  
Fusion Glycoprotein ◽  
Retrograde Gene Transfer

Application of highly efficient retrograde gene transfer vector for primate brain research

2011 ◽  
Vol 71 ◽  
pp. e41
Author(s):  
Kazuto Kobayashi ◽  
Shigeki Kato ◽  
Masahito Kuramochi ◽  
Kenta Kobayashi ◽  
Ken-ichi Inoue ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Brain Research ◽  
Transfer Vector ◽  
Highly Efficient ◽  
Primate Brain ◽  
Gene Transfer Vector ◽  
Retrograde Gene Transfer

scholarly journals Highly Efficient Retrograde Gene Transfer for Genetic Treatment of Neurological Diseases

10.5772/21992 ◽  
2011 ◽  
Author(s):  
Shigeki Kato ◽  
Masahito ◽  
Kenta Kobayashi ◽  
Ken-Ichi Inoue ◽  
Masahiko Takada ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Neurological Diseases ◽  
Highly Efficient ◽  
Retrograde Gene Transfer

scholarly journals Achieving highly efficient gene transfer to the bladder by increasing the molecular weight of polymer-based nanoparticles

2021 ◽  
Vol 332 ◽  
pp. 210-224
Author(s):  
Gang Li ◽  
Shanshan He ◽  
Andreas G. Schätzlein ◽  
Robert M. Weiss ◽  
Darryl T. Martin ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Transfer ◽  
Highly Efficient ◽  
Efficient Gene

Highly efficient cell-mediated gene transfer using non-viral vectors and FuGene™6: in vitro and in vivo studies

2000 ◽  
Vol 57 (8) ◽  
pp. 1326-1333 ◽  
Author(s):  
I. Hellgren* ◽  
V. Drvota ◽  
R. Pieper ◽  
S. Enoksson ◽  
P. Blomberg ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Viral Vectors ◽  
In Vivo Studies ◽  
Highly Efficient
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