scholarly journals Genetic Modification of Adeno-Associated Viral Vector Type 2 Capsid Enhances Gene Transfer Efficiency in Polarized Human Airway Epithelial Cells

2008 ◽  
Vol 19 (12) ◽  
pp. 1407-1414 ◽  
Author(s):  
April F. White ◽  
Marina Mazur ◽  
Eric J. Sorscher ◽  
Kurt R. Zinn ◽  
Selvarangan Ponnazhagan
Keyword(s):  
Gene Transfer ◽  
Epithelial Cells ◽  
Genetic Modification ◽  
Viral Vector ◽  
Airway Epithelial Cells ◽  
Transfer Efficiency ◽  
Airway Epithelial ◽  
Gene Transfer Efficiency ◽  
Vector Type

Genetic modification of AAV2 capsid enhances gene transfer efficiency in polarized human airway epithelial cells

2008 ◽  
Vol 0 (ja) ◽  
pp. 081015093227032
Author(s):  
April F White ◽  
Marina Mazur ◽  
Eric J Sorscher ◽  
Kurt Zinn ◽  
Selvarangan Ponnazhagan
Keyword(s):  
Gene Transfer ◽  
Epithelial Cells ◽  
Genetic Modification ◽  
Airway Epithelial Cells ◽  
Transfer Efficiency ◽  
Airway Epithelial ◽  
Gene Transfer Efficiency ◽  
Human Airway ◽  
Human Airway Epithelial Cells

Uptake of plasmid/glycosylated polymer complexes and gene transfer efficiency in differentiated airway epithelial cells

2002 ◽  
Vol 5 (1) ◽  
pp. 38-48 ◽  
Author(s):  
Isabelle Fajac ◽  
Guiti Thévenot ◽  
Laurent Bédouet ◽  
Claire Danel ◽  
Marc Riquet ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Epithelial Cells ◽  
Airway Epithelial Cells ◽  
Transfer Efficiency ◽  
Airway Epithelial ◽  
Polymer Complexes ◽  
Gene Transfer Efficiency

scholarly journals Limited Entry of Adenovirus Vectors into Well-Differentiated Airway Epithelium Is Responsible for Inefficient Gene Transfer

1998 ◽  
Vol 72 (7) ◽  
pp. 6014-6023 ◽  
Author(s):  
Raymond J. Pickles ◽  
Douglas McCarty ◽  
Hirotoshi Matsui ◽  
Pádraig J. Hart ◽  
Scott H. Randell ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Epithelial Cells ◽  
Adenovirus Vector ◽  
Airway Epithelial Cells ◽  
Transfer Efficiency ◽  
Apical Plasma Membrane ◽  
Airway Epithelial ◽  
Gene Transfer Efficiency ◽  
Well Differentiated

ABSTRACT Investigations of the efficiency and safety of human adenovirus vector (AdV)-mediated gene transfer in the airways of patients with cystic fibrosis (CF) in vivo have demonstrated little success in correcting the CF bioelectrical functional defect, reflecting the inefficiency of AdV-mediated gene transfer to the epithelial cells that line the airway luminal surface. In this study, we demonstrate that low AdV-mediated gene transfer efficiency to well-differentiated (WD) cultured airway epithelial cells is due to three distinct steps in the apical membrane of the airway epithelial cells: (i) the absence of specific adenovirus fiber-knob protein attachment receptors; (ii) the absence of αvβ3/5 integrins, reported to partially mediate the internalization of AdV into the cell cytoplasm; and (iii) the low rate of apical plasma membrane uptake pathways of WD airway epithelial cells. Attempts to increase gene transfer efficiency by increasing nonspecific attachment of AdV were unsuccessful, reflecting the inability of the attached vector to enter (penetrate) WD cells via nonspecific entry paths. Strategies to improve the efficiency of AdV for the treatment of CF lung disease will require methods to increase the attachment of AdV to and promote its internalization into the WD respiratory epithelium.

Dual-targeting non-viral vector based on polyethylenimine improves gene transfer efficiency

2007 ◽  
Vol 18 (5) ◽  
pp. 545-560 ◽  
Author(s):  
D. Li ◽  
G. P. Tang ◽  
J. Z. Li ◽  
Y. Kong ◽  
H. L. Huang ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Viral Vector ◽  
Transfer Efficiency ◽  
Gene Transfer Efficiency ◽  
Dual Targeting
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