Widespread Dispersion of Adeno-Associated Virus Serotype 1 and Adeno-Associated Virus Serotype 6 Vectors in the Rat Central Nervous System and in Human Glioblastoma Multiforme Xenografts

2005 ◽  
Vol 16 (3) ◽  
pp. 381-392 ◽  
Author(s):  
Peter C. Huszthy ◽  
Agnete Svendsen ◽  
James M. Wilson ◽  
Robert M. Kotin ◽  
Per Eystein Lønning ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Glioblastoma Multiforme ◽  
Adeno Associated Virus ◽  
Human Glioblastoma ◽  
Human Glioblastoma Multiforme ◽  
Virus Serotype ◽  
Serotype 1

scholarly journals Adeno-Associated Virus Serotype 9 Transduction in the Central Nervous System of Nonhuman Primates

2012 ◽  
Vol 23 (4) ◽  
pp. 382-389 ◽  
Author(s):  
Lluis Samaranch ◽  
Ernesto A. Salegio ◽  
Waldy San Sebastian ◽  
Adrian P. Kells ◽  
Kevin D. Foust ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Nonhuman Primates ◽  
Adeno Associated Virus ◽  
Virus Serotype ◽  
The Central Nervous System

scholarly journals Detection of dengue virus serotype 1 in central nervous system of a child in Bandung, West Java: A case report

2021 ◽  
Vol 9 ◽  
pp. 2050313X2110343
Author(s):  
Dewi Hawani Alisjahbana ◽  
Syndi Nurmawati ◽  
Dzulfikar DL Hakim ◽  
Mia Milanti ◽  
Yora Permata Dewi ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Dengue Virus ◽  
Clinical Symptoms ◽  
Central Nervous System Involvement ◽  
Immunoglobulin M ◽  
West Java ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Dengue Virus Serotype 1

Central nervous system involvement of dengue virus is increasingly reported from endemic areas. This study describes the clinical characteristics and laboratory features of a pediatric patient enrolled in a central nervous system illness study conducted in 2017–2018 to identify viral and bacterial etiologies in Indonesian children. Dengue diagnostics including molecular and serological testing were performed on an encephalitis patient who presented with both classical dengue and neurological clinical symptoms. Dengue virus serotype 1 RNA was detected in both cerebrospinal fluid and serum by serotype-specific reverse transcription polymerase chain reaction, and the E gene was successfully sequenced. Anti-dengue virus immunoglobulin M was detected in both admission and discharge sera, whereas anti-dengue virus immunoglobulin G was identified only in the discharge serum. This study describes the central nervous system complications in a case with dengue virus infection in West Java, Indonesia, and highlights the potential for dengue virus serotype 1, a serotype rarely associated with neurotropism, to cause encephalitis.

scholarly journals Dp71 Expression in Human Glioblastoma

2019 ◽  
Vol 20 (21) ◽  
pp. 5429 ◽  
Author(s):  
Simona Ruggieri ◽  
Michelina De Giorgis ◽  
Tiziana Annese ◽  
Roberto Tamma ◽  
Angelo Notarangelo ◽  
...  
Keyword(s):  
Nervous System ◽  
Glioblastoma Multiforme ◽  
Cell Lines ◽  
Glioblastoma Cell ◽  
Coding Region ◽  
Double Labeling ◽  
Human Glioblastoma ◽  
Lamin B ◽  
Human Glioblastoma Multiforme ◽  
Glioblastoma Cell Lines

Background: Dp71 is the most abundant dystrophin (DMD) gene product in the nervous system. Mutation in the Dp71 coding region is associated with cognitive disturbances in Duchenne muscular dystrophy (DMD) patients, but the function of dystrophin Dp71 in tumor progression remains to be established. This study investigated Dp71 expression in glioblastoma, the most common and aggressive primary tumor of the central nervous system (CNS). Methods: Dp71 expression was analyzed by immunofluorescence, immunohistochemistry, RT-PCR, and immunoblotting in glioblastoma cell lines and cells isolated from human glioblastoma multiforme (GBM) bioptic specimens. Results: Dp71 isoform was expressed in normal human astrocytes (NHA) cell lines and decreased in glioblastoma cell lines and cells isolated from human glioblastoma multiforme bioptic specimens. Moreover, Dp71 was localized in the nucleus in normal cells, while it was localized into the cytoplasm of glioblastoma cells organized in clusters. We have shown, by double labeling, that Dp71 colocalizes with lamin B in normal astrocytes cells, confirming the roles of Dp71 and lamin B in maintaining nuclear architecture. Finally, we demonstrated that decreased Dp71 protein in cells isolated from human bioptic specimens was inversely correlated with the Ki-67 tumor proliferative index. Conclusion: A decreased Dp71 expression is associated with cancer proliferation and poor prognosis in glioblastoma.

Transduction Profile of the Marmoset Central Nervous System Using Adeno-Associated Virus Serotype 9 Vectors

2016 ◽  
Vol 54 (3) ◽  
pp. 1745-1758 ◽  
Author(s):  
Yasunori Matsuzaki ◽  
Ayumu Konno ◽  
Ryo Mukai ◽  
Fumiaki Honda ◽  
Masafumi Hirato ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Adeno Associated Virus ◽  
Virus Serotype

Membranous stacks in human glioblastoma multiforme

1971 ◽  
Vol 18 (3) ◽  
pp. 248-256 ◽  
Author(s):  
Eiichi Tani ◽  
Toshio Ametani
Keyword(s):  
Glioblastoma Multiforme ◽  
Human Glioblastoma ◽  
Human Glioblastoma Multiforme

scholarly journals Lomeguatrib Increases the Radiosensitivity of MGMT Unmethylated Human Glioblastoma Multiforme Cell Lines

2021 ◽  
Vol 22 (13) ◽  
pp. 6781
Author(s):  
Anna Kirstein ◽  
Daniela Schilling ◽  
Stephanie E. Combs ◽  
Thomas E. Schmid
Keyword(s):  
Glioblastoma Multiforme ◽  
Cell Lines ◽  
Dna Methyltransferase ◽  
Treatment Options ◽  
Treatment Resistance ◽  
Cell Cycle Distribution ◽  
Human Glioblastoma ◽  
Human Glioblastoma Multiforme ◽  
Mgmt Protein

Background: Treatment resistance of glioblastoma multiforme to chemo- and radiotherapy remains a challenge yet to overcome. In particular, the O6-methylguanine-DNA-methyltransferase (MGMT) promoter unmethylated patients have only little benefit from chemotherapy treatment using temozolomide since MGMT counteracts its therapeutic efficacy. Therefore, new treatment options in radiotherapy need to be developed to inhibit MGMT and increase radiotherapy response. Methods: Lomeguatrib, a highly specific MGMT inhibitor, was used to inactivate MGMT protein in vitro. Radiosensitivity of established human glioblastoma multiforme cell lines in combination with lomeguatrib was investigated using the clonogenic survival assay. Inhibition of MGMT was analyzed using Western Blot. Cell cycle distribution and apoptosis were investigated to determine the effects of lomeguatrib alone as well as in combination with ionizing radiation. Results: Lomeguatrib significantly decreased MGMT protein and reduced radiation-induced G2/M arrest. A radiosensitizing effect of lomeguatrib was observed when administered at 1 µM and increased radioresistance at 20 µM. Conclusion: Low concentrations of lomeguatrib elicit radiosensitization, while high concentrations mediate a radioprotective effect.

scholarly journals NFATc1 activation promotes the invasion of U251 human glioblastoma multiforme cells through COX-2

2015 ◽  
Vol 35 (5) ◽  
pp. 1333-1340 ◽  
Author(s):  
LAIZANG WANG ◽  
ZHI WANG ◽  
JIANHUA LI ◽  
WEIGUANG ZHANG ◽  
FUBIN REN ◽  
...  
Keyword(s):  
Glioblastoma Multiforme ◽  
Human Glioblastoma ◽  
Human Glioblastoma Multiforme ◽  
Cox 2
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