scholarly journals Electrophoretic Component of Electric Pulses Determines the Efficacy of In Vivo DNA Electrotransfer

2005 ◽  
Vol 16 (10) ◽  
pp. 1194-1201 ◽  
Author(s):  
Saulius Šatkauskas ◽  
Franck André ◽  
Michel F. Bureau ◽  
Daniel Scherman ◽  
Damijan Miklavčič ◽  
...  
Keyword(s):  
Electric Pulses ◽  
Electrophoretic Component

scholarly journals Electrophoretic Component of Electric Pulses Determines the Efficacy of In Vivo DNA Electrotransfer

2005 ◽  
Vol 0 (0) ◽  
pp. 050926062340001 ◽  
Author(s):  
Saulius Satkauskas ◽  
Franck Andre ◽  
Michel F. Bureau ◽  
Daniel Scherman ◽  
Damijan Miklavcic ◽  
...  
Keyword(s):  
Electric Pulses ◽  
Electrophoretic Component

Adapting the Cassini Curve to Approximate In Vivo Irreversible Electroporation Ablations in Porcine Liver

2013 ◽  
Author(s):  
Paulo A. Garcia ◽  
Christopher B. Arena ◽  
Robert E. Neal ◽  
S. Nahum Goldberg ◽  
Eliel Ben-David ◽  
...  
Keyword(s):  
Cell Death ◽  
Transmembrane Potential ◽  
Tumor Tissue ◽  
Irreversible Electroporation ◽  
Low Energy ◽  
Porcine Liver ◽  
Electric Pulses ◽  
Focal Ablation ◽  
Increase Membrane Permeability

Irreversible electroporation (IRE) is a new minimally invasive non-thermal focal ablation technique that has been used for the treatment of spontaneous tumors in canine and human patients [1, 2]. The procedure typically involves placing two electrodes into or around a tumor and delivering a series of low energy electric pulses to kill tumor tissue with sub-millimeter resolution. The pulses generate an electric field that alters the resting transmembrane potential (TMP) of the cells. Depending on the magnitude of the induced TMP, the electric pulses can have no effect, reversibly increase membrane permeability, or cause cell death in the case of IRE.

scholarly journals Definition of a Novel Plasmid-Based Gene Transfection Protocol of Mammalian Skeletal Muscles by Means of In Vivo Electroporation

2020 ◽  
Vol 21 (18) ◽  
pp. 6494
Author(s):  
Enrico P. Spugnini ◽  
Manuel Scimeca ◽  
Bruno Amadio ◽  
Giancarlo Cortese ◽  
Maurizio Fanciulli ◽  
...  
Keyword(s):  
Gene Transfection ◽  
Dna Transfection ◽  
In Vivo Electroporation ◽  
Electric Pulses ◽  
Hind Limbs ◽  
Transfection Protocol ◽  
Gait Abnormalities ◽  
Definition Of ◽  
The Right

We describe an original electroporation protocol for in vivo plasmid DNA transfection. The right hind limbs of C57 mice are exposed to a specifically designed train of permeabilizing electric pulses by transcutaneous application of tailored needle electrodes, immediately after the injection of pEGFP-C1 plasmid encoding GFP (Green Fluorescente Protein). The electroporated rodents show a greater GFP expression than the controls at three different time points (4, 10, and 15 days). The electroporated muscles display only mild interstitial myositis, with a significant increase in inflammatory cell infiltrates. Finally, mild gait abnormalities are registered in electroporated mice only in the first 48 h after the treatment. This protocol has proven to be highly efficient in terms of expression levels of the construct, is easy to apply since it does not require surgical exposure of the muscle and is well tolerated by the animals because it does not cause evident morphological and functional damage to the electroporated muscle.

In Vivo Validation of Irreversible Electroporation Electric Field Threshold for Prostate Tissue

2013 ◽  
Author(s):  
Robert E. Neal ◽  
Helen Kavnoudias ◽  
Franklin Rosenfeldt ◽  
Ruchong Ou ◽  
James Marron ◽  
...  
Keyword(s):  
Thermal Ablation ◽  
Conventional Treatment ◽  
Irreversible Electroporation ◽  
Blood Perfusion ◽  
Complete Regression ◽  
Safety Study ◽  
Electric Pulses ◽  
Transmembrane Potentials ◽  
Focal Ablation

Irreversible electroporation (IRE) is a non-thermal focal ablation technique that uses needle electrodes to deliver a series of brief (100μs duration) electric pulses into the targeted region. These alter cellular transmembrane potentials, destabilizing the membranes in a manner that kills the cells while sparing major vasculature and other sensitive structures. IRE can therefore be used in regions ineligible for surgical resection or thermal ablation. Treatments result in rapid lesion creation and resolution [1], are unaffected by the blood perfusion “heat sink”, can be planned with numerical modeling [2], and its effects can be readily monitored with various imaging modalities [3]. Therapeutic ire has proven effective in the treatment of experimental [4] and clinical tumors. A human safety study attained complete regression in 46 of 69 tumors ineligible or unresponsive to conventional treatment [5], and veterinary case studies convey its utility in large difficult tumors [6, 7].

Targeted Gene Transfer to Corneal Stroma in vivo by Electric Pulses

2002 ◽  
Vol 74 (2) ◽  
pp. 191-198 ◽  
Author(s):  
Yuji Oshima ◽  
Taiji Sakamoto ◽  
Toshio Hisatomi ◽  
Chikako Tsutsumi ◽  
Yukio Sassa ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Corneal Stroma ◽  
Electric Pulses

Numerical Assessment of Thermal Response Associated With In Vivo Skin Electroporation: The Importance of the Composite Skin model

2006 ◽  
Vol 129 (3) ◽  
pp. 330-340 ◽  
Author(s):  
S. M. Becker ◽  
A. V. Kuznetsov
Keyword(s):  
Three Dimensional ◽  
Thermal Response ◽  
Physical Parameters ◽  
Damage Potential ◽  
Electric Pulses ◽  
Large Molecules ◽  
Composite Layers ◽  
Thermal Tissue Damage ◽  
Pass Through

Electroporation is an approach used to enhance transdermal transport of large molecules in which the skin is exposed to a series of electric pulses. The structure of the transport inhibiting outer layer, the stratum corneum, is temporarily destabilized due to the development of microscopic pores. Consequently agents that are ordinarily unable to pass into the skin are able to pass through this outer barrier. Of possible concern when exposing biological tissue to an electric field is thermal tissue damage associated with Joule heating. This paper shows the importance of using a composite model in calculating the electrical and thermal effects associated with skin electroporation. A three-dimensional transient finite-volume model of in vivo skin electroporation is developed to emphasize the importance of representing the skin’s composite layers and to illustrate the underlying relationships between the physical parameters of the composite makeup of the skin and resulting thermal damage potential.

Plate Electroporation: A Numerical Study of Factors Affecting Thermal Damage

2005 ◽  
Author(s):  
S. M. Becker ◽  
A. V. Kuznetsov
Keyword(s):  
Cell Membrane ◽  
Thermal Damage ◽  
Numerical Study ◽  
Three Dimensional ◽  
Physical Parameters ◽  
Damage Potential ◽  
Plate Electrode ◽  
Factors Affecting ◽  
Electric Pulses

Electroporation is an approach used to enhance the transport of large molecules to cell cytosol in which a targeted tissue region is exposed to a series of electric pulses. The cell membrane, which normally acts as a barrier to large molecule transport into the cell interior, is temporarily destabilized due to the development of pores in the cell membrane. Consequently agents that are ordinarily unable enter the cell are able to pass through the cell membrane. Of possible concern when exposing biological tissue to an electric field is thermal tissue damage associated with joule heating. This paper explores the thermal effects of various geometric, biological, and electroporation pulse parameters including the blood vessel presence and size, plate electrode configuration, and pulse duration and frequency. A three-dimensional transient finite volume model of in vivo parallel plate electroporation of liver tissue is used to develop a better understanding of the underlying relationships between the physical parameters involved with tissue electroporation and resulting thermal damage potential.

scholarly journals A Novel 3D Scaffold for Cell Growth to Asses Electroporation Efficacy

Cells ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 1470 ◽  
Author(s):  
Dettin ◽  
Sieni ◽  
Zamuner ◽  
Marino ◽  
Sgarbossa ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Cultures ◽  
Three Dimensional ◽  
Cell Interaction ◽  
Growth Patterns ◽  
3D Scaffold ◽  
Electric Pulses ◽  
Breast Carcinoma Cell Lines ◽  
Cell Cell

Tumor electroporation (EP) refers to the permeabilization of the cell membrane by means of short electric pulses thus allowing the potentiation of chemotherapeutic drugs. Standard plate adhesion 2D cell cultures can simulate the in vivo environment only partially due to lack of cell–cell interaction and extracellular matrix (ECM). In this study, we assessed a novel 3D scaffold for cell cultures based on hyaluronic acid and ionic-complementary self-assembling peptides (SAPs), by studying the growth patterns of two different breast carcinoma cell lines (HCC1569 and MDA-MB231). This 3D scaffold modulates cell shape and induces extracellular matrix deposit around cells. In the MDA-MB 231 cell line, it allows three-dimensional growth of structures known as spheroids, while in HCC1569 it achieves a cell organization similar to that observed in vivo. Interestingly, we were able to visualize the electroporation effect on the cells seeded in the new scaffold by means of standard propidium iodide assay and fluorescence microscopy. Thanks to the presence of cell–cell and cell–ECM interactions, the new 3D scaffold may represent a more reliable support for EP studies than 2D cancer cell cultures and may be used to test new EP-delivered drugs and novel EP protocols.

The effect of high frequency electric pulses on muscle contractions and antitumor efficiency in vivo for a potential use in clinical electrochemotherapy

2005 ◽  
Vol 65 (2) ◽  
pp. 121-128 ◽  
Author(s):  
Damijan Miklavčič ◽  
Gorazd Pucihar ◽  
Miran Pavlovec ◽  
Samo Ribarič ◽  
Marko Mali ◽  
...  
Keyword(s):  
High Frequency ◽  
Muscle Contractions ◽  
Electric Pulses ◽  
Potential Use
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