Hollow Waveguides for CO2 Laser Radiation in Operative Laparoscopy: An In Vitro Study

1998 ◽  
Vol 14 (3) ◽  
pp. 103-110 ◽  
Author(s):  
PETER H. COSSMANN ◽  
HANS J. ALTERMATT ◽  
EKKEHARD DREHER ◽  
STEFAN SPÖRRI
2015 ◽  
Vol 49 (6) ◽  
pp. 565-574 ◽  
Author(s):  
Marcella Esteves-Oliveira ◽  
Nadine Witulski ◽  
Ralf-Dieter Hilgers ◽  
Christian Apel ◽  
Hendrik Meyer-Lueckel ◽  
...  

The aim of this in vitro study was to evaluate the effect of combined CO2 laser and tin-containing fluoride treatment on the formation and progression of enamel erosive lesions. Ninety-six human enamel samples were obtained, stored in thymol solution and, after surface polishing, randomly divided into 6 different surface treatment groups (n = 16 in each group) as follows: no treatment, control (C); one CO2 laser irradiation (L1); two CO2 laser irradiations (L2); daily application of fluoride solution (F); combined daily fluoride solution + one CO2 laser irradiation (L1F), and combined daily fluoride solution + two CO2 laser irradiations (L2F). Laser irradiation was performed at 0.3 J/cm2 (5 µs/226 Hz/10.6 µm) on day 1 (L1) and day 6 (L2). The fluoride solution contained AmF/NaF (500 ppm F), and SnCl2 (800 ppm Sn) at pH 4.5. After surface treatment the samples were submitted to an erosive cycling over 10 days, including immersion in citric acid (2 min/0.05 M/pH = 2.3) 6 times daily and storage in remineralization solution (≥1 h) between erosive attacks. At the end of each cycling day, the enamel surface loss (micrometers) was measured using a 3D laser profilometer. Data were statistically analyzed by means of a 2-level mixed effects model and linear contrasts (α = 0.05). Group F (-3.3 ± 2.0 µm) showed significantly lower enamel surface loss than groups C (-27.22 ± 4.1 µm), L1 (-18.3 ± 4.4 µm) and L2 (-16.3 ± 5.3 µm) but higher than L1F (-1.0 ± 4.4 µm) and L2F (1.4 ± 3.2 µm, p < 0.05). Under the conditions of this in vitro study, the tin-containing fluoride solution caused 88% reduction of enamel surface loss, while its combination with CO2 laser irradiation at 0.3 J/cm2 hampered erosive loss almost completely.


Author(s):  
M Sadighi ◽  
R Pourabbas ◽  
H Mobaiyen ◽  
M Sadighi ◽  
S Rikhtegaran ◽  
...  

2002 ◽  
Vol 29 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Matthias Folwaczny ◽  
Albert Mehl ◽  
Hans Aggstaller ◽  
Reinhard Hickel

2016 ◽  
Author(s):  
Bruna Raquel Zancope ◽  
Vanessa B Dainezi ◽  
Marinês Nobre-dos-Santos ◽  
Sillas Duarte, Jr ◽  
Vanessa Pardi ◽  
...  

Background. CO2 laser has been used to morphologically and chemically modify the dental enamel surface as well as to turn it more resistant to demineralization. Despite a variety of experiments demonstrating the inhibitory effect of CO2 laser in reduce enamel demineralization, little is known about the effect of surface irradiated on bacterial growth. Thus, this in vitro study was preformed to evaluate the biofilm formation on enamel previously irradiated with a CO2 laser (λ = 10.6 µM). Methods. For this in vitro study, it was employed 96 specimens of bovine enamel, which were divided into 2 groups (n = 48): 1) Control-non-irradiated surface and 2) Irradiated enamel surface. Biofilms were grown on the enamel specimens by 1, 3 and 5 days under intermittent cariogenic condition in the irradiated and non irradiated surface. In each assessment time, the biofilm were evaluated by dry weigh, counting the number of viable colonies and in fifth day, were evaluated by polysaccharides analysis, quantitative real time PCR as well as by contact angle. In addition, the morphology of biofilms was characterized by fluorescence microscopy and field emission scanning electron microscopy (FESEM). Initially, the assumptions of equal variances and normal distribution of errors were conferred and the results are analyzed statistically by t-test and Mann Whitney test. Results. The mean of log CFU/ml obtained for the 1-day biofilm evaluation showed that there is statistical difference between the experimental groups. When biofilms were exposed to CO2 laser, CFU/mL and CFU/ Dry Weight in 3 day was reduced significantly compared with control group. The difference in the genes expression (gtfB and gbpB) and polysaccharides was not statically significant. Contact angle was increased relative to control when the surface was irradiated with CO2 laser. Similar morphology was also visible with both treatments, however irradiated group revealed evidence of melting and fusion in the specimens. Conclusion. In conclusion CO2 laser irradiation modify the energy surface and disrupt the initial biofilm formation.


1995 ◽  
Vol 13 (6) ◽  
pp. 397-400 ◽  
Author(s):  
A. MORITZ ◽  
N. GUTKNECHT ◽  
U. SCHOOP ◽  
J. WERNISCH ◽  
F. LAMPERT ◽  
...  

2008 ◽  
Vol 86 (Supplement) ◽  
pp. 357-358
Author(s):  
A Mehrsai ◽  
M Afsharpad ◽  
G Pourmand ◽  
B Nikbin ◽  
H Wahhabaghai ◽  
...  

2017 ◽  
Vol 35 (5) ◽  
pp. 282-286 ◽  
Author(s):  
Renato Siva Paulos ◽  
Priscila Yumi Seino ◽  
Karen Akemi Fukushima ◽  
Marcia Martins Marques ◽  
Fernanda Campos Sousa de Almeida ◽  
...  

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