Molecular Characterization of Prader–Willi Syndrome by Real-Time PCR

2008 ◽  
Vol 12 (2) ◽  
pp. 319-324 ◽  
Author(s):  
Teresa Munce ◽  
Robert Simpson ◽  
Francis Bowling
Keyword(s):  
Real Time ◽  
Molecular Characterization ◽  
Real Time Pcr ◽  
Prader Willi Syndrome

scholarly journals Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria

2020 ◽  
Vol 9 (1) ◽  
pp. 54
Author(s):  
Salem Belkessa ◽  
Daniel Thomas-Lopez ◽  
Karim Houali ◽  
Farida Ghalmi ◽  
Christen Rune Stensvold
Keyword(s):  
Real Time ◽  
Molecular Characterization ◽  
Real Time Pcr ◽  
Intestinal Parasites ◽  
Giardia Duodenalis ◽  
Specific Pcr ◽  
Stool Samples ◽  
Pcr Targeting ◽  
Assemblage B

The molecular epidemiology of giardiasis in Africa remains unclear. A study was carried out across four hospitals in Algeria. A total of 119 fecal samples from 55 children, 37 adults, and 27 individuals of undetermined age, all scored positive for intestinal parasites by microscopy, and were screened by real-time PCR for Giardia. Molecular characterization of Giardia was performed by assemblage-specific PCR and PCR targeting the triose phosphate isomerase gene (tpi). Of the 119 samples, 80 (67%) were Giardia-positive by real-time PCR. For 48 moderately-highly real-time PCR-positive samples, tpi genotyping assigned 22 samples to Assemblage A and 26 to Assemblage B. Contrary to Assemblage A, Assemblage B exhibited substantial genetic diversity and allelic heterozygosity. Assemblage-specific PCR proved to be specific for discriminating Assemblage A or B but not as sensitive as tpi genotyping. We confirmed that real-time PCR is more sensitive than microscopy for detecting Giardia in stool samples and that robust amplification and sequencing of the tpi gene is feasible when moderate-to-strongly real-time PCR-positive samples are used. This study is one of the few performed in Africa providing genotyping data on Giardia infections in humans. Both assemblages A and B were commonly seen and not associated with specific sociodemographic data.

scholarly journals Molecular characterization of circulating tumor cells in large quantities of contaminating leukocytes by a multiplex real-time PCR

2008 ◽  
Vol 118 (3) ◽  
pp. 455-468 ◽  
Author(s):  
Anieta M. Sieuwerts ◽  
Jaco Kraan ◽  
Joan Bolt-de Vries ◽  
Petra van der Spoel ◽  
Bianca Mostert ◽  
...  
Keyword(s):  
Real Time ◽  
Tumor Cells ◽  
Molecular Characterization ◽  
Circulating Tumor Cells ◽  
Real Time Pcr

Molecular Characterization of Large Deletions in the von Hippel-Lindau (VHL) Gene by Quantitative Real-Time PCR

2006 ◽  
Vol 10 (4) ◽  
pp. 243-249 ◽  
Author(s):  
Alberto Casarin ◽  
Maddalena Martella ◽  
Roberta Polli ◽  
Emanuela Leonardi ◽  
Laura Anesi ◽  
...  
Keyword(s):  
Real Time ◽  
Molecular Characterization ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr ◽  
Vhl Gene ◽  
Von Hippel Lindau ◽  
Large Deletions

Molecular characterization of atypical Chlamydia and evidence of their dissemination in different European and Asian chicken flocks by specific real-time PCR

2012 ◽  
Vol 14 (8) ◽  
pp. 2212-2222 ◽  
Author(s):  
Aleksandar Zocevic ◽  
Fabien Vorimore ◽  
Cvetka Marhold ◽  
Danijela Horvatek ◽  
Dongying Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Molecular Characterization ◽  
Real Time Pcr

Real-time PCR-based prevalence study, infection follow-up and molecular characterization of canine hemotropic mycoplasmas

2008 ◽  
Vol 126 (1-3) ◽  
pp. 132-141 ◽  
Author(s):  
Nicole Wengi ◽  
Barbara Willi ◽  
Felicitas S. Boretti ◽  
Valentino Cattori ◽  
Barbara Riond ◽  
...  
Keyword(s):  
Real Time ◽  
Molecular Characterization ◽  
Real Time Pcr ◽  
Prevalence Study

scholarly journals Multiplex Real-Time PCR Method for Simultaneous Identification and Toxigenic Type Characterization of Clostridium difficile From Stool Samples

2015 ◽  
Vol 35 (3) ◽  
pp. 306-313 ◽  
Author(s):  
Abdullah Kilic ◽  
Mohammad J. Alam ◽  
Naradah L. Tisdel ◽  
Dhara N. Shah ◽  
Mehmet Yapar ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Real Time ◽  
Real Time Pcr ◽  
Stool Samples ◽  
Pcr Method ◽  
Simultaneous Identification
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