Whole Genome Amplification on DNA from Filter Paper Blood Spot Samples: An Evaluation of Selected Systems

2007 ◽  
Vol 11 (1) ◽  
pp. 65-71 ◽  
Author(s):  
Karina Meden Sørensen ◽  
Cathrine Jespersgaard ◽  
Jens Vuust ◽  
David Hougaard ◽  
Bent Nørgaard-Pedersen ◽  
...  
Keyword(s):  
Filter Paper ◽  
Whole Genome Amplification ◽  
Whole Genome ◽  
Genome Amplification ◽  
Filter Paper Blood Spot ◽  
Blood Spot

scholarly journals Whole exome and whole genome sequencing with dried blood spot DNA without whole genome amplification

2017 ◽  
Vol 39 (1) ◽  
pp. 167-171 ◽  
Author(s):  
Laia Bassaganyas ◽  
George Freedman ◽  
Dedeepya Vaka ◽  
Eunice Wan ◽  
Richard Lao ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Whole Genome Amplification ◽  
Dried Blood Spot ◽  
Whole Genome ◽  
Genome Amplification ◽  
Whole Exome ◽  
Blood Spot

scholarly journals Quantifying genome DNA during whole-genome amplification via quantitative real-time multiple displacement amplification

RSC Advances ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 4617-4621
Author(s):  
Jing Tu ◽  
Yi Qiao ◽  
Yuhan Luo ◽  
Naiyun Long ◽  
Zuhong Lu
Keyword(s):  
Real Time ◽  
Whole Genome Amplification ◽  
Multiple Displacement Amplification ◽  
Whole Genome ◽  
Genome Amplification

Monitoring multiple displacement amplification by fluorescence signals.

Development of a Filter Paper Blood Spot Radioimmunoassay for Thyroid Stimulating Hormone Suitable for a Regional Neonatal Screening Unit

1983 ◽  
Vol 20 (2) ◽  
pp. 93-99 ◽  
Author(s):  
Hilary Moore ◽  
Mary McMillan
Keyword(s):  
Filter Paper ◽  
Neonatal Screening ◽  
Thyroid Stimulating Hormone ◽  
Paper Sample ◽  
Large Numbers ◽  
Critical Separation ◽  
Screening Unit ◽  
Guthrie Cards ◽  
Filter Paper Blood Spot ◽  
Blood Spot

The radioimmunoassay described measures TSH in dried whole blood spots collected from neonates onto filter paper Guthrie cards. Microgranular cellulose is added to the precipitating reagent at the critical separation stage of the assay to overcome imprecision caused by the presence of the filter paper sample disc in the tube. The method was developed for a regional neonatal screening unit and has been found to be very reliable during ten months' routine use. It was required to be as precise, sensitive, accurate, rapid, simple, and inexpensive as possible and suitable for use with automatic diluting equipment in order to process large numbers of samples. Other methods were examined for their suitability and found not to fulfil one or more of the above criteria.

scholarly journals Species Identification of Pinus Pollen Found in Belukha Glacier, Russian Altai Mountains, Using a Whole-Genome Amplification Method

Forests ◽  
2018 ◽  
Vol 9 (8) ◽  
pp. 444
Author(s):  
Fumio Nakazawa ◽  
Yoshihisa Suyama ◽  
Satoshi Imura ◽  
Hideaki Motoyama
Keyword(s):  
Species Identification ◽  
Whole Genome Amplification ◽  
Pollen Grains ◽  
Pollen Grain ◽  
Pinus Sibirica ◽  
Whole Genome ◽  
Accurate Identification ◽  
Closely Related Species ◽  
Genome Amplification ◽  
Amplification Method

Pollen taxa in sediment samples can be identified based on morphology. However, closely related species do not differ substantially in pollen morphology, and accurate identification is generally limited to genera or families. Because many pollen grains in glaciers contain protoplasm, genetic information obtained from pollen grains should enable the identification of plant taxa at the species level. In the present study, species identification of Pinus pollen grains was attempted using whole-genome amplification (WGA). We used pollen grains extracted from surface snow (depth, 1.8–1.9 m) from the Belukha glacier in the summer of 2003. WGA was performed using a single pollen grain. Some regions of the chloroplast genome were amplified by PCR, and the DNA products were sequenced to identify the pollen grain. Pinus includes approximately 111 recognized species in two subgenera, four sections, and 11 subsections. The tree species Pinus sibirica and P. sylvestris are currently found at the periphery of the glacier. We identified the pollen grains from the Belukha glacier to the level of section or subsection to which P. sibirica and P. sylvestris belong. Moreover, we specifically identified two pollen grains as P. sibirica or P. cembra. Fifteen species, including P. sibirica, were candidates for the remaining pollen grain.

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