Rapid Detection of Common Southeast Asian ß-Thalassemia Mutations by Nonisotopic Multiplex PCR-SSCP Analysis

2004 ◽  
Vol 8 (2) ◽  
pp. 104-108
Author(s):  
S.P. Yip ◽  
L.F. Fung ◽  
S.T.H. Lo
Keyword(s):  
Multiplex Pcr ◽  
Rapid Detection ◽  
Southeast Asian ◽  
Sscp Analysis

Rapid Detection of Extended Spectrum β-Lactamase (ESBL) for Enterobacteriaceae by use of a Multiplex PCR-based Method

2009 ◽  
Vol 41 (3) ◽  
pp. 181 ◽  
Author(s):  
Junyoung Kim ◽  
Semi Jeon ◽  
Hogeun Rhie ◽  
Bokkwon Lee ◽  
Misun Park ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Rapid Detection ◽  
Extended Spectrum

scholarly journals Multiplex PCR Assay for Rapid Detection and Genotyping of Helicobacter pylori Directly from Biopsy Specimens

2004 ◽  
Vol 42 (6) ◽  
pp. 2821-2824 ◽  
Author(s):  
S. Chattopadhyay ◽  
R. Patra ◽  
T. Ramamurthy ◽  
A. Chowdhury ◽  
A. Santra ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Multiplex Pcr ◽  
Rapid Detection ◽  
Pcr Assay ◽  
Biopsy Specimens ◽  
Multiplex Pcr Assay

scholarly journals Rapid Detection of Virulent Salmonella Strains Using Multiplex PCR

2012 ◽  
Vol 11 (19) ◽  
pp. 3544-3549
Author(s):  
Zengqi Yang ◽  
Li Qiu ◽  
Chao Sun ◽  
Hu Yang ◽  
Chunli Li ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Rapid Detection

Development of multiplex PCR assay for rapid detection Listeria monocytogenes in clinical samples

2021 ◽  
Vol 30 (4) ◽  
pp. 20-26
Author(s):  
Le Thanh Huong ◽  
Ha Thi Phuong Mai ◽  
Hoang Thi Thu Ha ◽  
Nguyen Dong Tu ◽  
Bui Tien Sy ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Multiplex Pcr ◽  
Rapid Detection ◽  
Pathogenic Bacteria ◽  
Vulnerable Groups ◽  
Clinical Samples ◽  
Specific Gene ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Specificity And Sensitivity

Listeria monocytogenes is widely present in the natural environment. This bacteria can cause infections in both humans and animals. In humans, the most vulnerable groups to be infected with L. monocytogenes are the elderly, people with an impaired immune system and chronically illness, pregnant women, and newborn babies. The aim of this study was to develop a multiplex PCR assay for the rapid detection of L. monocytogenes in mock clinical samples. A pair of primers were designed for detection of L. monocytogenes based on prs, a Listeria genus specific gene, and hly, a hemolysin gene. The specificity of the primers were tested by using different L. monocytogenes strains and other common pathogenic bacteria. The results showed that L. monocytogenes strains were positive in the detection and other tested strains were negative in mock (spiked) clinical samples. The sensitivity of multiplex PCR assay was 102 CFU/ml per reaction. The specificity and sensitivity of multiplex PCR technology for detecting L. monocytogenes in mock (spiked) clinical samples were high, and the assay could be completed within 1.5 hours. Therefore, this established multiplex PCR provides a rapid and reliable method and will be useful for the detection of L. monocytogenes in mock clinical samples.

scholarly journals Rapid Detection of Shiga Toxin-Producing Bacteria in Feces by Multiplex PCR with Molecular Beacons on the Smart Cycler

2002 ◽  
Vol 40 (4) ◽  
pp. 1436-1440 ◽  
Author(s):  
S. D. Belanger ◽  
M. Boissinot ◽  
C. Menard ◽  
F. J. Picard ◽  
M. G. Bergeron
Keyword(s):  
Multiplex Pcr ◽  
Shiga Toxin ◽  
Rapid Detection ◽  
Molecular Beacons

Rapid detection of Listeria monocytogenes sequence type 121 strains using a novel multiplex PCR assay

LWT ◽  
2019 ◽  
Vol 116 ◽  
pp. 108474 ◽  
Author(s):  
Moutong Chen ◽  
Jianheng Cheng ◽  
Rui Pang ◽  
Jumei Zhang ◽  
Yuetao Chen ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Multiplex Pcr ◽  
Rapid Detection ◽  
Sequence Type ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

Single-tube multiplex PCR-SSCP analysis distinguishes 7 common ABO alleles and readily identifies new alleles

Blood ◽  
2000 ◽  
Vol 95 (4) ◽  
pp. 1487-1492 ◽  
Author(s):  
Shea Ping Yip
Keyword(s):  
Multiplex Pcr ◽  
Blood Group ◽  
Single Strand Conformation Polymorphism ◽  
Rare Allele ◽  
Sscp Analysis ◽  
Intragenic Recombination ◽  
Blood Group System ◽  
Single Tube ◽  
Conformation Polymorphism ◽  
New Alleles

The ABO blood group is clinically the most important blood group system. Elucidation of the molecular basis of the ABO polymorphism allows genotype determination without family studies. Described here is a new method based on the simultaneous amplification by polymerase chain reaction (PCR) of 3 fragments from exon 6, and 5′ and 3′ ends of exon 7 of the ABO gene, followed by single-strand conformation polymorphism (SSCP) analysis. This multiplex PCR-SSCP protocol allows the well-established base changes at 9 nucleotide positions 261, 297, 467, 526, 646, 657, 681, 1059, and 1096 to be assayed simultaneously so that 7 common alleles (A1, A1v, A2, B, O1, O1v, and O2) can be distinguished in a single-tube single-lane format. Each allele was characterized by a set of 3 haplotype-specific SSCP patterns. Chinese (n = 125) and white European (n = 98) samples were analyzed, and their genotypes were found consistent with the serologic phenotypes or could be deduced unambiguously. Fifteen samples (2 Chinese and 13 white European) were each found carrying at least 1 rare allele. Most of these alleles were new and some might be generated by intragenic recombination. This technique is the simplest, quickest, and most informative method reported to date and also readily identifies new alleles.

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