Upstream Intensification with High-Density Cell Cryopreservation

2020 ◽  
Vol 40 (4) ◽  
pp. 21-21
Keyword(s):  
High Density ◽  
High Density Cell

High density cell culture ofRhodotorula glutinis using oxygen-enriched air

1986 ◽  
Vol 8 (10) ◽  
pp. 715-718 ◽  
Author(s):  
Jae Gu Pan ◽  
Moo Young Kwak ◽  
Joon Shick Rhee
Keyword(s):  
Cell Culture ◽  
High Density ◽  
High Density Cell ◽  
High Density Cell Culture

scholarly journals High-Density Cell Arrays for Genome-Scale Phenotypic Screening

2019 ◽  
Vol 24 (3) ◽  
pp. 274-283 ◽  
Author(s):  
Vytaute Starkuviene ◽  
Stefan M. Kallenberger ◽  
Nina Beil ◽  
Tautvydas Lisauskas ◽  
Bastian So-Song Schumacher ◽  
...  
Keyword(s):  
New Technologies ◽  
High Density ◽  
Cell Array ◽  
Preclinical Development ◽  
Single Experiment ◽  
Efficient Manner ◽  
Cell Arrays ◽  
High Density Cell ◽  
High Degree ◽  
Genome Scale

Due to high associated costs and considerable time investments of cell-based screening, there is a strong demand for new technologies that enable preclinical development and tests of diverse biologicals in a cost-saving and time-efficient manner. For those reasons we developed the high-density cell array (HD-CA) platform, which miniaturizes cell-based screening in the form of preprinted and ready-to-run screening arrays. With the HD-CA technology, up to 24,576 samples can be tested in a single experiment, thereby saving costs and time for microscopy-based screening by 75%. Experiments on the scale of the entire human genome can be addressed in a real parallel manner, with screening campaigns becoming more comfortable and devoid of robotics infrastructure on the user side. The high degree of miniaturization enables working with expensive reagents and rare and difficult-to-obtain cell lines. We have also optimized an automated imaging procedure for HD-CA and demonstrate the applicability of HD-CA to CRISPR-Cas9- and RNAi-mediated phenotypic assessment of the gene function.

scholarly journals The mitogenic effect of platelet-derived growth factor in human hepatic stellate cells requires calcium influx

1995 ◽  
Vol 269 (5) ◽  
pp. C1133-C1139 ◽  
Author(s):  
P. Failli ◽  
C. Ruocco ◽  
R. De Franco ◽  
A. Caligiuri ◽  
A. Gentilini ◽  
...  
Keyword(s):  
Growth Factor ◽  
Cell Cultures ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
High Density ◽  
Platelet Derived Growth Factor ◽  
Low Density ◽  
Plateau Phase ◽  
Time Dynamics ◽  
High Density Cell

Platelet-derived growth factor (PDGF) is a key mitogen for hepatic stellate cells (HSC) and has been shown to be implicated in liver tissue repair and fibrogenesis. In this study the relationship between PDGF-induced intracellular Ca2+ concentration ([Ca2+]i) increase and mitogenesis in cultured human HSC was evaluated. In high-density cell cultures (80-90% subconfluence), PDGF induced a significant increase in [Ca2+]i, characterized by a short-lasting peak phase, which was followed by a long-lasting plateau phase. The plateau phase was abolished in the absence of extracellular Ca2+. However, in low-density cell cultures (30-40% subconfluence), the plateau phase was absent or markedly less pronounced. In parallel sets of experiments, PDGF was significantly less effective in inducing mitogenesis in low-density cell cultures than in high-density cell cultures and was totally ineffective in the absence of extracellular Ca2+. These results suggest that 1) spatial and time dynamics of PDGF-induced [Ca2+]i increase are dependent on cell density and 2) PDGF-induced mitogenesis requires extracellular Ca2+ influx.

A method for the design of 3D scaffolds for high-density cell attachment and determination of optimum perfusion culture conditions

2008 ◽  
Vol 41 (7) ◽  
pp. 1436-1449 ◽  
Author(s):  
Christophe Provin ◽  
Kiyoshi Takano ◽  
Yasuyuki Sakai ◽  
Teruo Fujii ◽  
Ryo Shirakashi
Keyword(s):  
Cell Attachment ◽  
Perfusion Culture ◽  
Culture Conditions ◽  
High Density ◽  
3D Scaffolds ◽  
High Density Cell
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