New England Biolabs Looks Past Enzymes

2013 ◽  
Vol 33 (6) ◽  
pp. 08-09
Author(s):  
Alex Philippidis
Keyword(s):  
New England ◽  
England Biolabs

High Throughput Semi-Automated SARS-CoV-2 Library Preparation Protocol for Ion Torrent Sequencing using Opentrons, New England Biolabs Kit, and ARTIC Primers v2

protocols.io ◽  
2021 ◽  
Author(s):  
Elias Dahdouh ◽  
Fernando Lázaro Perona ◽  
María Rodríguez Tejedor ◽  
Rubén Cáceres Sánchez ◽  
Iván Bloise Sánchez ◽  
...  
Keyword(s):  
New England ◽  
High Throughput ◽  
Ion Torrent ◽  
Library Preparation ◽  
England Biolabs ◽  
Ion Torrent Sequencing ◽  
Library Preparation Protocol

Preface to the Proceedings of the New England Biolabs Workshop on Biological DNA Modification

Gene ◽  
1988 ◽  
Vol 74 (1) ◽  
pp. 1-2 ◽  
Author(s):  
R.M. Blumenthal ◽  
S. Hattman ◽  
J.E. Brooks ◽  
E.A. Raleigh ◽  
W. Szybalski
Keyword(s):  
New England ◽  
Dna Modification ◽  
England Biolabs

scholarly journals Report on Overcoming the Poor Quality of ApaI Pulsotypes with a Short Review on PFGE for Listeria monocytogenes

2013 ◽  
Vol 62 (3) ◽  
pp. 307-309 ◽  
Author(s):  
DARJA KUŠAR ◽  
MAJA KAVALIČ ◽  
MATJAŽ OCEPEK ◽  
IRENA ZDOVC
Keyword(s):  
Public Health ◽  
Listeria Monocytogenes ◽  
New England ◽  
Short Review ◽  
Epidemiological Studies ◽  
Poor Quality ◽  
Restriction Digestion ◽  
The Poor ◽  
England Biolabs

Since listeriosis, caused by Listeria monocytogenes, is one of the important concerns of public health in Europe related to foodborne zoonoses, an efficient protocol for isolate typing is necessary when performing epidemiological studies. Three standardized PFGE protocols available for L. monocytogenes were briefly reviewed. Since observing a poor-quality of ApaI pulsotypes in our laboratory, enzymes from three different manufacturers were compared. The obtained pulsotypes showed that restriction digestion with ApaI from New England BioLabs should be complemented with a subsequent overnight incubation of PFGE plugs in TE buffer for better performance.

scholarly journals CLONING AND EXPRESSION OF THE IPT GENE

HortScience ◽  
1992 ◽  
Vol 27 (11) ◽  
pp. 1160e-1160
Author(s):  
Sandra L. Barbour ◽  
D.A. Schaff ◽  
J.J. Frett
Keyword(s):  
New England ◽  
Cloning And Expression ◽  
Isopentenyl Transferase ◽  
Shoot Initiation ◽  
Purification System ◽  
England Biolabs ◽  
Cytokinin Synthesis ◽  
Polymerase Chain ◽  
Future Work

Cytokinins are involved in in vitro shoot initiation, although little is known about their mode of action. As the first step in localizing cytokinin synthesis, we present a cloning and expression strategy for the isopentenyl transferase (ipt) gene. The source of the Agrobacterium tumefaciens ipt gene was a 7.2 kb Eco RI fragment isolated from pBREF7 (Dr. Ann Smigocki, USDA, Beltsville, MD). The ipt gene was amplified by polymerase chain reaction (PCR) and cloned into pMal-cRI (New England Biolabs, Beverly, MA). Using the pMAL-cRI expression and purification system, the isopentenyl transferase protein will be purified for antibody production. These antibodies will be used in future work to localize the isopentenyl transferase enzyme.

scholarly journals InBase, the New England Biolabs Intein Database

1999 ◽  
Vol 27 (1) ◽  
pp. 346-347 ◽  
Author(s):  
F. B. Perler
Keyword(s):  
New England ◽  
England Biolabs
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