Footprint-Free, Site-Specific Gene Editing

2013 ◽  
Vol 33 (5) ◽  
pp. 18-19
Author(s):  
Carlisle Landel
Keyword(s):  
Gene Editing ◽  
Specific Gene ◽  
Site Specific

Abstract 1154: Site-specific gene editing with ABBIE for T-cell therapy

2021 ◽  
Author(s):  
Jihyun Park ◽  
Xiaohong Wang ◽  
Leonardo Mirandola ◽  
Maurizio Chiriva-Internati
Keyword(s):  
T Cell ◽  
Cell Therapy ◽  
Gene Editing ◽  
Specific Gene ◽  
T Cell Therapy ◽  
Site Specific

scholarly journals Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA

2015 ◽  
Vol 169 (2) ◽  
pp. 931-945 ◽  
Author(s):  
Sergei Svitashev ◽  
Joshua K. Young ◽  
Christine Schwartz ◽  
Huirong Gao ◽  
S. Carl Falco ◽  
...  
Keyword(s):  
Gene Editing ◽  
Targeted Mutagenesis ◽  
Specific Gene ◽  
Site Specific ◽  
Guide Rna ◽  
Gene Insertion

scholarly journals 612. Site-Specific Gene Editing of COL7A1 Restores Type VII Collagen in RDEB iPSCs

2015 ◽  
Vol 23 ◽  
pp. S243
Author(s):  
Christos Georgiadis ◽  
Anastasia Petrova ◽  
John A. McGrath ◽  
Adrian J. Thrasher ◽  
Wei-Li Di ◽  
...  
Keyword(s):  
Gene Editing ◽  
Specific Gene ◽  
Site Specific ◽  
Type Vii Collagen

scholarly journals Induced mutagenesis in wheat: from ionizing radiation to site-specific gene editing

2021 ◽  
Vol 53 (1) ◽  
pp. 29-54
Author(s):  
O. Kishchenko ◽  
◽  
A. Stepanenko ◽  
M. Borisjuk ◽  
◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
Gene Editing ◽  
Specific Gene ◽  
Site Specific ◽  
Induced Mutagenesis

scholarly journals CRISPR/Cas in genome defense and gene editing

2016 ◽  
Vol 9 (1) ◽  
pp. 68-74
Author(s):  
Svetlana Kryštofová
Keyword(s):  
Gene Editing ◽  
Defense Mechanism ◽  
Cell Types ◽  
Eukaryotic Cells ◽  
Specific Gene ◽  
Gene Expressions ◽  
Site Specific ◽  
Genome Defense ◽  
Engineered Nucleases ◽  
Foreign Dna

AbstractTargeted genome editing using engineered nucleases such as ZFNs and TALENs has been rapidly replaced by the CRISPR/Cas9 (clustered, regulatory interspaced, short palindromic/ CRISPR-associated nuclease) system. CRISPR/Cas9 technology represents a significant improvement enabling a new level of targeting, efficiency and simplicity. Gene editing mediated by CRISPR/Cas9 has been recently used not only in bacteria but in many eukaryotic cells and organisms, from yeasts to mammals. Other modifications of the CRISPR-Cas9 system have been used to introduce heterologous domains to regulate gene expressions or label specific loci in various cell types. The review focuses not only on native CRISPR/Cas systems which evolved in prokaryotes as an endogenous adaptive defense mechanism against foreign DNA attacks, but also on the CRISPR/Cas9 adoption as a powerful tool for site-specific gene modifications in fungi, plants and mammals.

scholarly journals Site-Specific Gene Editing of Human Hematopoietic Stem Cells for X-Linked Hyper-IgM Syndrome

Cell Reports ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2606-2616 ◽  
Author(s):  
Caroline Y. Kuo ◽  
Joseph D. Long ◽  
Beatriz Campo-Fernandez ◽  
Satiro de Oliveira ◽  
Aaron R. Cooper ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Gene Editing ◽  
Specific Gene ◽  
Hematopoietic Stem ◽  
Site Specific ◽  
Hyper Igm Syndrome ◽  
Hyper Igm

scholarly journals Peptide Nucleic Acids as a Tool for Site-Specific Gene Editing

Molecules ◽  
2018 ◽  
Vol 23 (3) ◽  
pp. 632 ◽  
Author(s):  
Adele Ricciardi ◽  
Elias Quijano ◽  
Rachael Putman ◽  
W. Saltzman ◽  
Peter Glazer
Keyword(s):  
Nucleic Acids ◽  
Gene Editing ◽  
Peptide Nucleic Acids ◽  
Specific Gene ◽  
Site Specific

scholarly journals Exploiting DNA Endonucleases to Advance Mechanisms of DNA Repair

Biology ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 530
Author(s):  
Marlo K. Thompson ◽  
Robert W. Sobol ◽  
Aishwarya Prakash
Keyword(s):  
Dna Repair ◽  
Mammalian Cells ◽  
Genome Stability ◽  
Gene Editing ◽  
Adaptive Immune System ◽  
Zinc Finger Nucleases ◽  
Specific Gene ◽  
Target Sequence ◽  
Transcription Activator ◽  
Low Cytotoxicity

The earliest methods of genome editing, such as zinc-finger nucleases (ZFN) and transcription activator-like effector nucleases (TALENs), utilize customizable DNA-binding motifs to target the genome at specific loci. While these approaches provided sequence-specific gene-editing capacity, the laborious process of designing and synthesizing recombinant nucleases to recognize a specific target sequence, combined with limited target choices and poor editing efficiency, ultimately minimized the broad utility of these systems. The discovery of clustered regularly interspaced short palindromic repeat sequences (CRISPR) in Escherichia coli dates to 1987, yet it was another 20 years before CRISPR and the CRISPR-associated (Cas) proteins were identified as part of the microbial adaptive immune system, by targeting phage DNA, to fight bacteriophage reinfection. By 2013, CRISPR/Cas9 systems had been engineered to allow gene editing in mammalian cells. The ease of design, low cytotoxicity, and increased efficiency have made CRISPR/Cas9 and its related systems the designer nucleases of choice for many. In this review, we discuss the various CRISPR systems and their broad utility in genome manipulation. We will explore how CRISPR-controlled modifications have advanced our understanding of the mechanisms of genome stability, using the modulation of DNA repair genes as examples.

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