Effectiveness of a Direct-Fed Microbial Product Containing Lactobacillus acidophilus and Lactobacillus casei in Reducing Fecal Shedding of Escherichia coli O157:H7 in Commercial Feedlot Cattle

2020 ◽  
Author(s):  
Diana M.A. Dewsbury ◽  
Natalia Cernicchiaro ◽  
Brandon Depenbusch ◽  
T.G. Nagaraja ◽  
David G. Renter
Keyword(s):  
Escherichia Coli ◽  
Lactobacillus Acidophilus ◽  
Lactobacillus Casei ◽  
Feedlot Cattle ◽  
Escherichia Coli O157 ◽  
Fecal Shedding ◽  
Microbial Product

Effect of Lactobacillus acidophilus Strain NP51 on Escherichia coli O157:H7 Fecal Shedding and Finishing Performance in Beef Feedlot Cattle†

2007 ◽  
Vol 70 (2) ◽  
pp. 287-291 ◽  
Author(s):  
R. E. PETERSON ◽  
T. J. KLOPFENSTEIN ◽  
G. E. ERICKSON ◽  
J. FOLMER ◽  
S. HINKLEY ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lactobacillus Acidophilus ◽  
Repeated Measures ◽  
Immunomagnetic Separation ◽  
Feedlot Cattle ◽  
Outcome Variable ◽  
Escherichia Coli O157 ◽  
Fecal Shedding ◽  
Selective Enrichment ◽  
E Coli

A 2-year study was conducted during the summer months (May to September) to test the effectiveness of feeding Lactobacillus acidophilus strain NP51 on the proportion of cattle shedding Escherichia coli O157:H7 in the feces and evaluate the effect of the treatment on finishing performance. Steers (n = 448) were assigned randomly to pens, and pens of cattle were assigned randomly to NP51 supplementation or no supplementation (control). NP51 products were mixed with water and applied as the feed was mixed daily in treatment-designated trucks at the rate of 109 CFU per steer. Fecal samples were collected (n = 3,360) from the rectum from each animal every 3 weeks, and E. coli O157:H7 was isolated by standard procedures, using selective enrichment, immunomagnetic separation, and PCR confirmation. The outcome variable was the recovery of E. coli O157:H7 from feces, and was modeled using logistic regression accounting for year, repeated measures of pens of cattle, and block. No significant differences were detected for gain, intakes, or feed efficiency of control or NP51-fed steers. The probability for cattle to shed E. coli O157:H7 varied significantly between 2002 and 2003 (P = 0.004). In 2002 and 2003, the probability for NP51-treated steers to shed E. coli O157:H7 over the test periods was 13 and 21%, respectively, compared with 21 and 28% among controls. Over the 2 years, NP51-treated steers were 35% less likely to shed E. coli O157: H7 than were steers in untreated pens (odds ratio = 0.58, P = 0.008). This study is consistent with previous reports that feeding NP51 is effective in reducing E. coli O157:H7 fecal shedding in feedlot cattle.

Evaluation of Commercial β-Agonists, Dietary Protein, and Shade on Fecal Shedding of Escherichia coli O157:H7 from Feedlot Cattle

2017 ◽  
Vol 14 (11) ◽  
pp. 649-655 ◽  
Author(s):  
James E. Wells ◽  
Elaine D. Berry ◽  
Minseok Kim ◽  
Steven D. Shackelford ◽  
Kristin E. Hales
Keyword(s):  
Escherichia Coli ◽  
Dietary Protein ◽  
Feedlot Cattle ◽  
Escherichia Coli O157 ◽  
Fecal Shedding

Fecal Shedding of Escherichia coli O157:H7 in North Dakota Feedlot Cattle in the Fall and Spring

2006 ◽  
Vol 69 (5) ◽  
pp. 1154-1158 ◽  
Author(s):  
MARGARET L. KHAITSA ◽  
MARC L. BAUER ◽  
GREGORY P. LARDY ◽  
DAWN K. DOETKOTT ◽  
REDEMPTA B. KEGODE ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
North Dakota ◽  
The United States ◽  
Extreme Weather ◽  
Early Spring ◽  
Feedlot Cattle ◽  
Escherichia Coli O157 ◽  
Fecal Shedding ◽  
E Coli

Cattle are an important reservoir of Escherichia coli O157:H7, which can lead to contamination of food and water, and subsequent human disease. E. coli O157:H7 shedding in cattle has been reported as seasonal, with more animals shedding during summer and early fall than during winter. North Dakota has relatively cold weather, especially in winter and early spring, compared with many other regions of the United States. The objective was to assess fecal shedding of E. coli O157: H7 in North Dakota feedlot cattle over the fall, winter, and early spring. One hundred forty-four steers were assigned randomly to 24 pens on arrival at the feedlot. Samples of rectal feces were obtained from each steer four times (October and November 2003, and March and April 2004) during finishing. On arrival (October 2003), 2 (1.4%) of 144 cattle were shedding E. coli O157:H7. The shedding increased significantly to 10 (6.9%) of 144 after 28 days (November 2003), to 76 (53%) of 143 at the third sampling (March 2004), and dropped significantly to 30 (21%) of 143 at the fourth (last) sampling (March 2004) before slaughter. Unfortunately, we were unable to sample the cattle during winter because of the extreme weather conditions. Sampling time significantly (P < 0.0001) influenced variability in E. coli O157:H7 shedding, whereas herd (P = 0.08) did not. The prevalence of E. coli O157:H7 shedding in North Dakota steers in fall and early spring was comparable to what has been reported in other parts of the United States with relatively warmer weather. Further research into E. coli O157:H7 shedding patterns during extreme weather such as North Dakota winters is warranted in order to fully assess the seasonal effect on the risk level of this organism.

scholarly journals Efficacy of a vaccine and a direct-fed microbial against fecal shedding of Escherichia coli O157:H7 in a randomized pen-level field trial of commercial feedlot cattle

Vaccine ◽  
2012 ◽  
Vol 30 (43) ◽  
pp. 6210-6215 ◽  
Author(s):  
Charley A. Cull ◽  
Zachary D. Paddock ◽  
T.G. Nagaraja ◽  
Nora M. Bello ◽  
Abram H. Babcock ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Field Trial ◽  
Feedlot Cattle ◽  
Escherichia Coli O157 ◽  
Fecal Shedding

Naturally Colonized Beef Cattle Populations Fed Combinations of Yeast Culture and an Ionophore in Finishing Diets Containing Dried Distiller's Grains with Solubles Had Similar Fecal Shedding of Escherichia coli O157:H7

2011 ◽  
Vol 74 (6) ◽  
pp. 912-918 ◽  
Author(s):  
K. L. SWYERS ◽  
B. A. CARLSON ◽  
K. K. NIGHTINGALE ◽  
K. E. BELK ◽  
S. L. ARCHIBEQUE
Keyword(s):  
Escherichia Coli ◽  
Dietary Treatment ◽  
Feedlot Cattle ◽  
Yeast Culture ◽  
Escherichia Coli O157 ◽  
Fecal Shedding ◽  
E Coli ◽  
Pfge Typing ◽  
Multiplex Pcr Assay ◽  
Water Trough

Beef steers (n = 252) were used to evaluate the effects of dietary supplement on fecal shedding of Escherichia coli O157:H7. Seven pens of 9 steers (63 steers per treatment) were fed diets supplemented with or without yeast culture (YC) or monensin (MON) and their combination (YC × MON). YC and MON were offered at 2.8 g/kg and 33 mg/kg of dry matter intake, respectively. Environmental sponge samples (from each pen floor, feed bunk, and water trough) were collected on day 0. Rectal fecal grab samples were collected on days 0, 28, 56, 84, 110, and 125. Samples were collected and pooled by pen and analyzed for presumptive E. coli O157:H7 colonies, which were confirmed by a multiplex PCR assay and characterized by pulsed-field gel electrophoresis (PFGE) typing. On day 0, E. coli O157:H7 was detected in 7.0% of feed bunk samples and 14.3% of pen floor samples but in none of the water trough samples. The 71.4% prevalence of E. coli O157:H7 in fecal samples on day 0 decreased significantly (P < 0.05) over time. E. coli O157:H7 fecal shedding was not associated with dietary treatment (P > 0.05); however, in cattle fed YC and YC × MON fecal shedding was 0% by day 28. Eight XbaI PFGE subtypes were identified, and a predominant subtype and three closely related subtypes (differing by three or fewer bands) accounted for 78.7% of environmental and fecal isolates characterized. Results from this study indicate that feeding YC to cattle may numerically decrease but not eliminate fecal shedding of E. coli O157:H7 at the onset of treatment and that certain E. coli O157 subtypes found in the feedlot environment may persist in feedlot cattle.

Comparative Effect of Direct-Fed Microbials on Fecal Shedding of Escherichia coli O157:H7 and Salmonella in Naturally Infected Feedlot Cattle

2008 ◽  
Vol 71 (3) ◽  
pp. 539-544 ◽  
Author(s):  
EBOT S. TABE ◽  
JAMES OLOYA ◽  
DAWN K. DOETKOTT ◽  
MARC L. BAUER ◽  
PENELOPE S. GIBBS ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Feedlot Cattle ◽  
Control Group ◽  
Escherichia Coli O157 ◽  
Comparative Effect ◽  
Laboratory Methods ◽  
Fecal Shedding ◽  
E Coli ◽  
Feedlot Steers ◽  
And Control

The effect of direct-fed microbials (DFM) on fecal shedding of Escherichia coli O157:H7 and Salmonella in naturally infected feedlot cattle was evaluated in a clinical trial involving 138 feedlot steers. Following standard laboratory methods, fecal samples collected from steers were evaluated for change in the detectable levels of E. coli O157:H7 and Salmonella shed in feces after DFM treatment. Sampling of steers was carried out every 3 weeks for 84 days. A significant reduction (32%) in fecal shedding of E. coli O157:H7 (P < 0.001), but not Salmonella (P = 0.24), was observed among the treatment steers compared with the control group during finishing. The probability of recovery of E. coli O157:H7 from the feces of treated and control steers was 34.0 and 66.0%, respectively. Steers placed on DFM supplement were almost three times less likely to shed E. coli O157:H7 (odds ratio, 0.36; 95% confidence interval, 0.25 to 0.53; P < 0.001) in their feces as opposed to their control counterparts. The probability of recovery of Salmonella from the feces of the control (14.0%) and the treated (11.3%) steers was similar. However, the DFM significantly reduced probability of new infections with Salmonella among DFM-treated cattle compared with controls (nontreated ones). It appears that DFM as applied in our study are capable of significantly reducing fecal shedding of E. coli O157:H7 in naturally infected cattle but not Salmonella. The factors responsible for the observed difference in the effects of DFM on E. coli O157:H7 and Salmonella warrants further investigation.

Evaluation of a Direct-Fed Microbial Product Effect on the Prevalence and Load of Escherichia coli O157:H7 in Feedlot Cattle†

2010 ◽  
Vol 73 (2) ◽  
pp. 366-371 ◽  
Author(s):  
TERRANCE M. ARTHUR ◽  
JOSEPH M. BOSILEVAC ◽  
NORASAK KALCHAYANAND ◽  
JAMES E. WELLS ◽  
STEVEN D. SHACKELFORD ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Intervention Strategy ◽  
Feedlot Cattle ◽  
Subtilis Strain ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Microbial Product ◽  
Significant Difference ◽  
Antimicrobial Intervention ◽  
And Control

Direct-fed microbials (DFM) have been identified as potential preharvest interventions for the reduction of foodborne bacterial pathogens such as Escherichia coli O157:H7. This study evaluated the efficacy of a DFM consisting of Bacillus subtilis strain 166 as an antimicrobial intervention strategy for the reduction of prevalence and load of E. coli O157:H7 in feces and on hides of feedlot cattle. Cattle (n = 526) were divided among 16 feedlot pens. Half of the pens received the DFM, and the other half did not. Hide and fecal samples were collected from each animal on days 28, 63, and 84 of the feeding trial. Over the course of the 84-day feeding period, there were no significant differences observed between treatments for either hide or fecal prevalence of E. coli O157:H7, or for the percentage of animals that were shedding E. coli O157:H7 at high levels (≥200 CFU/g) in their feces or harboring E. coli O157:H7 at high levels (≥40 CFU/cm2) on their hides. In addition, there was no significant difference between the average daily gains for the treated and control groups, with both groups averaging 1.3 kg/day. We concluded that the DFM tested would not be an effective preharvest intervention against E. coli O157:H7.

Effect of Ractopamine HCl Supplementation on Fecal Shedding of Escherichia coli O157:H7 and Salmonella in Feedlot Cattle

2006 ◽  
Vol 53 (4) ◽  
pp. 340-345 ◽  
Author(s):  
T. S. Edrington ◽  
T. R. Callaway ◽  
S. E. Ives ◽  
M. J. Engler ◽  
T. H. Welsh ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Feedlot Cattle ◽  
Escherichia Coli O157 ◽  
Fecal Shedding

Influence of transportation stress and animal temperament on fecal shedding of Escherichia coli O157:H7 in feedlot cattle

Meat Science ◽  
2009 ◽  
Vol 81 (2) ◽  
pp. 300-306 ◽  
Author(s):  
C.E. Schuehle Pfeiffer ◽  
D.A. King ◽  
L.M. Lucia ◽  
E. Cabrera-Diaz ◽  
G.R. Acuff ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Feedlot Cattle ◽  
Escherichia Coli O157 ◽  
Fecal Shedding ◽  
Transportation Stress

Prevalence and Enumeration of Escherichia coli O157 in Steers Receiving Various Strains of Lactobacillus-Based Direct-Fed Microbials

2007 ◽  
Vol 70 (5) ◽  
pp. 1252-1255 ◽  
Author(s):  
T. P. STEPHENS ◽  
G. H. LONERAGAN ◽  
L. M. CHICHESTER ◽  
M. M. BRASHEARS
Keyword(s):  
Escherichia Coli ◽  
Lactobacillus Acidophilus ◽  
Immunomagnetic Separation ◽  
Feedlot Cattle ◽  
Most Probable Number ◽  
Escherichia Coli O157 ◽  
Feeding Period ◽  
Probable Number ◽  
Selective Enrichment ◽  
E Coli

The objective of this research was to evaluate the effect of daily dietary inclusion of specific strains of Lactobacillus acidophilus on prevalence and concentration of Escherichia coli O157 in harvest-ready feedlot cattle. Five hundred yearling steers were housed in pens of 10 animals each. At arrival, steers were randomly allocated to one of five cohorts. Four of the cohorts were fed various strains and dosages of Lactobacillus-based direct-fed microbials throughout the feeding period. Fecal samples were collected from the rectum of each animal immediately prior to shipment to the abattoir. E. coli O157 was detected using selective enrichment and immunomagnetic separation techniques. For positive samples, E. coli O157 concentration was estimated using a most-probable-number (MPN) technique that included immunomagnetic separation. Prevalence varied among the cohorts (P < 0.01). The prevalence in the controls (26.3%) was greater (P < 0.05) than that in cattle supplemented with L. acidophilus strains NP51, NP28, or NP51-NP35 (13.0, 11.0, and 11.0%, respectively). The greatest E. coli O157 concentration was also observed in the controls (3.2 log MPN/g of feces); this concentration was greater (P < 0.05) than that observed in positive animals receiving NP51, NP28, or NP51-NP35 (0.9, 1.1, 1.7 log MPN/g of feces, respectively). Specific strains of Lactobacillus-based direct-fed microbials effectively reduced the prevalence and concentration of E. coli O157 in harvest-ready cattle, whereas others did not. When using direct-fed microbials to reduce carriage of E. coli O157 in cattle, it is important to select appropriately validated products.

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