scholarly journals Isolation and Characterization of Antimicrobial-Resistant Escherichia coli from Retail Meats from Roadside Butcheries in Uganda

2020 ◽  
Vol 17 (11) ◽  
pp. 666-671
Author(s):  
Torahiko Okubo ◽  
Montira Yossapol ◽  
Shiori Ikushima ◽  
Steven Kakooza ◽  
Eddie M. Wampande ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Isolation And Characterization ◽  
Retail Meats

Isolation and Characterization of the Shiga Toxin Gene (stx)–Bearing Escherichia coli O157 and Non-O157 from Retail Meats in Shandong Province, China, and Characterization of the O157-Derived stx2 Phages

2008 ◽  
Vol 71 (4) ◽  
pp. 706-713 ◽  
Author(s):  
TSUTOMU KOITABASHI ◽  
SHAN CUI ◽  
MUHAMMAD KAMRUZZAMAN ◽  
MITSUAKI NISHIBUCHI
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Shandong Province ◽  
Toxin Gene ◽  
Immunomagnetic Bead ◽  
E Coli ◽  
Isolation And Characterization ◽  
Retail Meats ◽  
Q Gene

Infection by Shiga toxin (Stx)–producing Escherichia coli of non-O157 and O157 serotypes are rare in China, but infection by O157 serotype was found in Shandong Province and three other provinces in China. To understand the reason for these rare infections and to determine the safety of retail meats in Shandong Province, we examined the distribution of Shiga toxin gene (stx)–bearing E. coli in retail meats and characterized the isolated stx-bearing strains. We used hybridization with DNA probes and isolated stx1- and/or stx2-positive E. coli from 31 (58%) of 53 retail meat samples, with beef showing the highest frequency (68%). Of 42 stx-positive isolates, none belonged to O157. Using the O157-specific immunomagnetic bead technique, we isolated E. coli O157 carrying the eae and stx2 genes from eight beef samples (26%). These strains produced little or no Stx2 and carried a unique q gene. Replication of the stx2 phages was detected in these strains, whereas stx2 phage replication was not detected in our previous study in which we examined similar stx2-bearing E. coli O157 strains from other Asian countries. Analysis of E. coli C600 lysogenized with the stx2 phages found in this study suggests that the lack of Stx2 production is due to changes in non-q gene region(s) of the phage genome or chromosomal mutation(s) in the host. Our data and reports by other workers suggest it is necessary to determine if various stx2-bearing E. coli O157 strains producing Stx2 to varying degrees are distributed in meats in various locations in China.

Isolation and characterization of a novel Escherichia coli Kayfunavirus phage DY1

2021 ◽  
Vol 293 ◽  
pp. 198274
Author(s):  
Xiaoming Yuan ◽  
Shuhong Zhang ◽  
Juan Wang ◽  
Chun Li ◽  
Na Li ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of pathogenic Escherichia coli bacteriophages from chicken and beef offal

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Celosia Lukman ◽  
Christopher Yonathan ◽  
Stella Magdalena ◽  
Diana Elizabeth Waturangi
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogens ◽  
High Efficiency ◽  
Multiplicity Of Infection ◽  
Isolation And Characterization ◽  
Transmission Electron ◽  
Pathogenic Escherichia Coli ◽  
Family Based

Abstract Objective This study was conducted to isolate and characterize lytic bacteriophages for pathogenic Escherichia coli from chicken and beef offal, and analyze their capability as biocontrol for several foodborne pathogens. Methods done in this research are bacteriophage isolation, purification, titer determination, application, determination of host range and minimum multiplicity of infection (miMOI), and bacteriophage morphology. Results Six bacteriophages successfully isolated from chicken and beef offal using EPEC and EHEC as host strain. Bacteriophage titers observed between 109 and 1010 PFU mL−1. CS EPEC and BL EHEC bacteriophage showed high efficiency in reduction of EPEC or EHEC contamination in meat about 99.20% and 99.04%. The lowest miMOI was 0.01 showed by CS EPEC bacteriophage. CI EPEC and BL EPEC bacteriophage suspected as Myoviridae family based on its micrograph from Transmission Electron Microscopy (TEM). Refers to their activity, bacteriophages isolated in this study have a great potential to be used as biocontrol against several foodborne pathogens.

Isolation and characterization of the heat stable enterotoxin from a pathogenic bovine strain of Escherichia coli

1984 ◽  
Vol 9 (4) ◽  
pp. 399-414 ◽  
Author(s):  
Charles Gerday ◽  
Marianne Herman ◽  
Jacques Olivy ◽  
Nicole Gerardin-Otthiers ◽  
Dominique Art ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Heat Stable ◽  
Isolation And Characterization ◽  
Bovine Strain
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