scholarly journals Development and In-House Validation of a Real-Time Polymerase Chain Reaction for the Detection of Listeria monocytogenes in Meat

2018 ◽  
Vol 15 (1) ◽  
pp. 55-57 ◽  
Author(s):  
Camilo Reyes ◽  
Luciano H. Linares ◽  
Fabiana Moredo ◽  
Juan P. Lirón ◽  
Victoria Brusa ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Listeria Monocytogenes ◽  
Real Time ◽  
Chain Reaction ◽  
Polymerase Chain

Rapid Enumeration of Listeria monocytogenes in Artificially Contaminated Cabbage Using Real-Time Polymerase Chain Reaction

2002 ◽  
Vol 65 (8) ◽  
pp. 1329-1332 ◽  
Author(s):  
ANGELA J. HOUGH ◽  
SALLY-ANN HARBISON ◽  
MARION G. SAVILL ◽  
LAURENCE D. MELTON ◽  
GRAHAM FLETCHER
Keyword(s):  
Polymerase Chain Reaction ◽  
Listeria Monocytogenes ◽  
Real Time ◽  
Chain Reaction ◽  
Organic Solvent Extraction ◽  
Rapid Enumeration ◽  
Good Potential ◽  
Quantitative Results ◽  
Polymerase Chain ◽  
Solvent Extraction Technique

A quantitative real-time polymerase chain reaction (PCR) detection method specific for Listeria monocytogenes was developed, and studies involving pure culture showed that the response of the assay was linear over 7 log10 (log) cycles. The method was then applied to the detection of L. monocytogenes artificially inoculated onto cabbage, a vegetable chosen because it is a major component of coleslaw, which has been associated with an outbreak of listeriosis. After being allowed to attach to the food, cells were washed from the cabbage leaf surface and recovered by centrifugation. The DNA was purified by an organic solvent extraction technique and analyzed by real-time PCR. In this matrix, the method again produced a linear response over 7 log cycles from 1.4 × 102 to 1.4 × 109 CFU of L. monocytogenes in 25 g of cabbage, and analysis of the reproducibility of the system showed that log differences in L. monocytogenes numbers added to cabbage could be reliably distinguished. The system allowed quantitative results to be obtained within 8 h and was relatively inexpensive, showing good potential for routine analytical use.

scholarly journals Polymerase Chain Reaction-Based Methods for Detection of Listeria monocytogenes: Toward Real-Time Screening for Food and Environmental Samples

2002 ◽  
Vol 85 (2) ◽  
pp. 505-515 ◽  
Author(s):  
Dawn M Norton
Keyword(s):  
Polymerase Chain Reaction ◽  
Listeria Monocytogenes ◽  
Real Time ◽  
Environmental Samples ◽  
Messenger Rna ◽  
Nucleic Acid Amplification ◽  
Screening Methods ◽  
Chain Reaction ◽  
Assay Sensitivity ◽  
Polymerase Chain

Abstract A review is presented of nucleic acid amplification-based methodology, specifically polymerase chain reaction (PCR)-based assays, for the detection of Listeria monocytogenes in food and environmental samples. Until recently, developmental challenges including poor sensitivity, due in part to reaction inhibition by components of the sample matrix, and the potential for false-positive reactions have limited routine application of PCR-based screening assays. Commercial assays address these challenges while offering convenient, standardized protocols, a high level of automation, and results within 2 days after the sampling date. Although sample enrichment is necessary to achieve desired detection limits, continued efforts toward template purification will facilitate the development of assays offering real-time, quantitative results. The development of ribonucleic acid (RNA) amplification-based assays may increase in importance, particularly if end-product testing is prioritized by regulatory agencies, as messenger RNA appears to serve as an accurate indicator of cell viability. Further, the increase in target copy number may improve assay sensitivity. PCR-based screening methods offer efficient, reliable results and are ideal for monitoring the presence of L. monocytogenes in foods and in the food processing environment.

1504: Molecular Diagnosis and Staging of Prostate Cancer Using Clusterin in Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)

2006 ◽  
Vol 175 (4S) ◽  
pp. 485-486
Author(s):  
Sabarinath B. Nair ◽  
Christodoulos Pipinikas ◽  
Roger Kirby ◽  
Nick Carter ◽  
Christiane Fenske
Keyword(s):  
Prostate Cancer ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Molecular Diagnosis ◽  
Reverse Transcription ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain
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