scholarly journals Effect of the Enrichment Medium on the Detection and Diversity ofSalmonellafrom Porcine Duodenal Content

2013 ◽  
Vol 10 (2) ◽  
pp. 182-188 ◽  
Author(s):  
Emily V. De Busser ◽  
Dominiek Maes ◽  
Kurt Houf ◽  
Jeroen Dewulf ◽  
Lieven De Zutter
Keyword(s):  
Duodenal Content ◽  
Enrichment Medium

Clostridium difficilein Healthy Adults: Evaluation of Carriage Using an Enrichment Medium

1989 ◽  
Vol 2 (3) ◽  
Author(s):  
M. Cerquetti ◽  
A. Pantosti ◽  
L. Grieco ◽  
P. Mastrantonio
Keyword(s):  
Healthy Adults ◽  
Enrichment Medium

A case of fatal drug intoxication showing a high-density duodenal content by postmortem computed tomography

2011 ◽  
Vol 13 (1) ◽  
pp. 39-40 ◽  
Author(s):  
Rie Sano ◽  
Keiko Takahashi ◽  
Yoshihiko Kominato ◽  
Takuya Araki ◽  
Koujiro Yamamoto ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Duodenal Content ◽  
High Density ◽  
Postmortem Computed Tomography ◽  
Drug Intoxication

scholarly journals Selective Enrichment Medium for Paratyphoid Bacteria1

1959 ◽  
Vol 7 (2) ◽  
pp. 63-66 ◽  
Author(s):  
F. Rappaport ◽  
N. Konforti
Keyword(s):  
Enrichment Medium ◽  
Selective Enrichment

scholarly journals A comparison of the original Rappaport medium (R medium) and the Rappaport–Vassiliadis medium (RV medium) in the isolation of salmonellae from meat products

1984 ◽  
Vol 93 (1) ◽  
pp. 51-58 ◽  
Author(s):  
P. Vassiliadis ◽  
V. Kalapothaki ◽  
Ch. Mavrommati ◽  
D. Trichopoulos
Keyword(s):  
Meat Products ◽  
Enrichment Medium

SUMMARYThe Rappaport–Vassiliadis enrichment medium (RV medium)in 10 ml quantities (RV/43 °C, 10 ml) inoculated with 0·1 ml of pre-enrichment medium (P medium) was found more efficient in the isolation of salmonellae from 409 pre-enriched samples (mainly meat products), than the original Rappaport medium incubated at 43 °C (R/43 °C) and the RV medium in 5 ml quantities (RV/43 °C, 5 ml) inoculated with 0·01 ml of P medium (P< 0·001, in both instances). Therefore, the inoculum from pre-enriched foods should not be less than 0·l ml in 10 ml of RV medium.The RV/43°, 10 ml was also better (P< 0·01) in detecting samples containing salmonellas than the original Rappaport medium incubated at 37 °C (R/37 °C, 10 ml) and the modification R25 of R medium incubated at 37 °C. The R25 modification was used in 10 ml quantities (R25/37 °C, 10 ml) inoculated with 0·1 ml of P medium and in 5 ml quantities (R25/37°, 5 ml) inoculated with 0·01 ml of P medium. The last two R25 procedures were of the same efficiency in isolating salmonellas from meat products.

scholarly journals Multiplex detection of nine food-borne pathogens by mPCR and capillary electrophoresis after using a universal pre-enrichment medium

2015 ◽  
Vol 6 ◽  
Author(s):  
Germán Villamizar-Rodríguez ◽  
Javier Fernández ◽  
Laura Marín ◽  
Juan Muñiz ◽  
Isabel González ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Multiplex Detection ◽  
Enrichment Medium ◽  
Food Borne Pathogens ◽  
Food Borne

Comparison of Rappaport-Vassiliadis Enrichment Medium and Tetrathionate Brilliant Green Broth for Isolation of Salmonellae from Meat Products

1983 ◽  
Vol 46 (7) ◽  
pp. 618-621 ◽  
Author(s):  
V. KALAPOTHAKI ◽  
P. VASSILIADIS ◽  
CH. MAVROMMATI ◽  
D. TRICHOPOULOS
Keyword(s):  
Brilliant Green ◽  
Meat Products ◽  
Enrichment Medium ◽  
Peptone Water ◽  
Salmonella Serotypes ◽  
Green Solution

The effectiveness of Rappaport-Vassiliadis enrichment medium (RV medium) and Difco's tetrathionate brilliant green broth (TBG) for detection of Salmonella in 553 samples of meat products was compared. All samples were preenriched for 20 h in buffered peptone water. Then 0.1 ml of the preenrichment was inoculated into 10 ml of RV medium, 1 ml was added to 9 ml of TBG broth, and 1 ml was inoculated into 10 ml of Muller-Kauffman (MK) tetrathionate broth. All enrichments were incubated at 43°C for 24 h, except for MK broth which was incubated for 48 h, and all were subcultured onto brilliant green deoxycholate agar and bismuth sulfite agar. The Rappaport-Vassiliadis medium was superior to Difco's tetrathionate brilliant green broth, being considerably more sensitive and more specific. The superiority of RV medium concerned the number of positive samples (36% and 28%, respectively), and also the number of Salmonella serotypes and strains. The RV medium inhibited the lactose- and sucrose-negative competing organisms much more than the Difco's tetrathionate broth. The performance of Difco and Muller-Kauffman tetrathionate brilliant green broths was similar. Addition of the brilliant green solution after boiling the tetrathionate broth slightly increased its efficacy. The effectiveness of brilliant green deoxycholate agar and bismuth sulfite agar was similar, whether after enrichment in RV medium or in any of the studied tetrathionate brilliant green broths.

Absence of Luminal Bile Increases Duodenal Content of Cholecystokinin in Rats

1991 ◽  
Vol 197 (2) ◽  
pp. 175-180 ◽  
Author(s):  
K. Miyasaka ◽  
A. Funakoshi ◽  
M. Matsumoto ◽  
A. Jimi ◽  
F. Shikado ◽  
...  
Keyword(s):  
Duodenal Content

scholarly journals The number of bacilli harboured by enteric carriers

1954 ◽  
Vol 52 (1) ◽  
pp. 67-70 ◽  
Author(s):  
Scott Thomson
Keyword(s):  
Pure Culture ◽  
Medical Officer ◽  
Salmonella Typhi ◽  
Enrichment Medium ◽  
Mental Hospitals ◽  
Culture Plate ◽  
Large Numbers ◽  
Paratyphi B ◽  
Almost All

Salmonella typhi or S. paratyphi B were found in very large numbers in the faeces of enteric carriers. Of twenty-four carriers, four were found negative by the fullest examination. Of the twenty found positive almost all harboured many millions of bacilli per gram of faeces.A minute inoculum of one drop (1/50 ml.) of a 1:1000 dilution of faeces on a culture plate only rarely failed to reveal all the positives without the use of an ‘enrichment’ medium and the result of such a procedure was a culture plate with virtually a pure culture of the pathogen.I am grateful to the Medical Superintendents of the Mental Hospitals at Cardiff, Bridgend and Denbigh, and the Medical Officer of Health for Brecon for submitting specimens for examination.

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