scholarly journals Foodborne Pathogens in Milk and the Dairy Farm Environment: Food Safety and Public Health Implications

2005 ◽  
Vol 2 (2) ◽  
pp. 115-129 ◽  
Author(s):  
S.P. Oliver ◽  
B.M. Jayarao ◽  
R.A. Almeida
2014 ◽  
Vol 77 (1) ◽  
pp. 100-105 ◽  
Author(s):  
MUHSIN AYDIN ◽  
GENE P. D. HERZIG ◽  
KWANG CHEOL JEONG ◽  
SAMANTHA DUNIGAN ◽  
PARTH SHAH ◽  
...  

Escherichia coli O157:H7 is a major foodborne pathogen that has posed serious problems for food safety and public health. Recent outbreaks and recalls associated with various foods contaminated by E. coli O157:H7 clearly indicate its deleterious effect on food safety. A rapid and sensitive detection assay is needed for this harmful organism to prevent foodborne illnesses and control outbreaks in a timely manner. We developed a magnetic bead–based immunoassay for detection of E. coli O157:H7 (the most well-known Shiga toxigenic E. coli strain) with a 96-well microplate as an assay platform. Immunomagnetic separation (IMS) and tyramide signal amplification were coupled to the assay to increase its sensitivity and specificity. This immunoassay was able to detect E. coli O157:H7 in pure culture with a detection limit of 50 CFU/ml in less than 3 h without an enrichment step. The detection limit was decreased 10-fold to 5 CFU/ml with addition of a 3-h enrichment step. When this assay was tested with other nontarget foodborne pathogens and common enteric bacteria, no cross-reactivity was found. When tested with artificially contaminated ground beef and milk samples, the assay sensitivity decreased two- to fivefold, with detection limits of 250 and 100 CFU/ml, respectively, probably because of the food matrix effect. The assay results also were compared with those of a sandwich-type enzyme-linked immunosorbent assay (ELISA) and an ELISA coupled with IMS; the developed assay was 25 times and 4 times more sensitive than the standard ELISA and the IMS-ELISA, respectively. Tyramide signal amplification combined with IMS can improve sensitivity and specificity for detection of E. coli O157:H7. The developed assay could be easily adapted for other foodborne pathogens and will contribute to improved food safety and public health.


Author(s):  
Linda Verrill ◽  
A. Lando ◽  
F. Wu ◽  
A Tatavarthy ◽  
Donald Obenhuber

Several outbreaks of shigatoxin-producing E. coli (STEC) in the past decade linked to flour and flour-associated products have raised concerns that the consumption of raw flour represents a public health risk as a vehicle for foodborne pathogens. The extent to which consumers know and understand they should not consume raw flour is unclear. In the fall of 2019, the U.S. Food and Drug Administration collected data on uncooked flour perceptions and self-reported consumption behaviors via the Food Safety and Nutrition Survey (FSANS), a national probability survey of U.S. adults (18+). Cross-tabulations and regressions were used to analyze the data (n=2,171). Thirty-five percent of consumers reported having tasted or eaten something with uncooked flour in it in the last 12 months. Responses differed significantly by sex, race, education, and age. On average, respondents indicated that uncooked flour is not likely to contain germs that can make people sick, with significant differences noted by demographic categories. Respondents rated raw homemade cookie dough as moderately likely to have germs that can make people sick, with significant demographic differences. In conclusion, U.S. consumers are largely unaware that raw flour is risky to consume, and a sizeable number are consuming products that contain raw flour.


2016 ◽  
Vol 8 (37) ◽  
pp. 6668-6681 ◽  
Author(s):  
Yuqian Jiang ◽  
Shan Zou ◽  
Xudong Cao

Identification and quantification of foodborne pathogens are becoming increasingly important to public health and food safety since the majority of foodborne illnesses and deaths are caused by pathogenic bacteria.


2020 ◽  
Vol 1 (2) ◽  
pp. 98-103
Author(s):  
C. O. Vinayananda ◽  
Mohamed Nadeem Fairoze ◽  
C. B. Madhavaprasad ◽  
Nagappa S. K.

Abstract: Foods are analyzed for food safety hazards in order to comply with food safety standards. Among food-borne pathogens Salmonella enterica and Listeria monocytogenes are recognized as major foodborne pathogens of public health significance worldwide. In the present study, multiplex polymerase chain reaction (mPCR) was used to screen for S. enterica and L. monocytogenes in table eggs collected from different markets (840 eggs). Pooled egg samples categorized as commercial and backyard eggs based on the source of collection. Collected eggs were screened for S. enterica and L. monocytogenes using mPCR assays. Conserved regions viz. invA and prfA genes were targeted for the specific detection of S. enterica and L. monocytogenes, respectively. mPCR and conventional method showed same results, prevalence of S. enterica at 12.5 and 33.3 per cent in commercial and backyard eggs, respectively. While, L. monocytogenes was undetectable in commercial eggs; but, detected only in backyard table egg sample (8.3%). Present study indicated complete concordance between specific pre-enrichment mPCR and conventional cultural methods. Results of the study underscored mPCR as steadfast rapid tool for the screening of table eggs for listed food safety hazards S. enterica and L. monocytogenes in table eggs. Keywords: Eggs; Listeria; mPCR; Public Health; Salmonella


Author(s):  
Seungjun Lee ◽  
Chulkyoon Mok ◽  
Jiyoung Lee

Once pathogens are internalized in fresh produce, they pose a challenging food safety issue since they are not effectively inactivated by conventional rinsing or sanitization. To protect food safety and public health, the objectives were to examine internalized levels of foodborne pathogens in different types of fresh produce and to investigate the effectiveness of photocatalytically enhanced inactivation of internalized pathogens in fresh produce using UV irradiation with titanium dioxide (TiO 2 ). For this, green fluorescent protein-labeled S. Typhimurium and E. coli O157:H7 were inoculated on the leaf surface of four types of fresh produce (~10 8 CFU (colony-forming unit)/leaf) and varying concentrations of TiO 2 suspension (0.50, 0.75, 1.00, 1.25, and 1.50 µg/ml)) were applied to the surface of contaminated leaves. Depending on the nature of each vegetable, the internalized bacterial level differed (log 2 – 5 CFU/g of leaf). When UV irradiation (6,000 J/m 2 ) was applied, the internalized S. Typhimurium and E. coli levels were reduced by 0.8 – 2.4 log CFU/leaf and was with TiO 2 , the reduction was 1.1 – 3.7 log CFU/leaf. The inactivation efficiency increased as the TiO 2 concentration (up to 1.50 μg/leaf). These results indicate that the TiO 2 application enhanced the photocatalytic inactivation of internalized foodborne pathogens. The application of TiO2 would be most practical before UV irradiation and before distributing the produce. This study established a platform for future research on the inactivation of various internalized pathogens for protecting public health and scaling up fresh produce treatments by the food industry.


Author(s):  
Lois M. Davis ◽  
Nancy Nicosia ◽  
Adrian Overton ◽  
Lisa Miyashiro ◽  
Kathryn Pitkin Derose ◽  
...  

2011 ◽  
Author(s):  
T. W. Meeks ◽  
I. V. Vahia ◽  
H. Lavretsky ◽  
G. Kulkarni ◽  
D. V. Jeste

Sign in / Sign up

Export Citation Format

Share Document